Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effect of Saponin Content and Antioxidant Activities of Platycodon grandiflorum Radix by Cutting Length

도라지 절단 길이에 따른 사포닌 함량 및 항산화 활성

  • Lee, Byung Jin (Research Center for Seed Utilization of Gyeongnam National University of Science and Technology) ;
  • Jeon, Seung Ho (Research Center for Seed Utilization of Gyeongnam National University of Science and Technology) ;
  • No, Il Rae (Department of Agronomy Gyeongsang National University) ;
  • Kim, Young Guk (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Cho, Young Son (Department of Agronomy and Medicinal Plant Resourses, Gyeongnam National University of Science and Technology)
  • 이병진 (경남과학기술대학교 종자실용화연구소) ;
  • 전승호 (경남과학기술대학교 종자실용화연구소) ;
  • 노일래 (경상대학교 농업식물과학과) ;
  • 김영국 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 조영손 (경남과학기술대학교 농학.한약자원학부)
  • Received : 2015.06.12
  • Accepted : 2015.09.03
  • Published : 2015.10.30
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Abstract

Background : Platycodon grandiflorum radix used as a therapeutic agent for lung and respiratory diseases in traditional Korean medicine. The saponin and sugar content in P. grandiflorum root is dependent on the drying temperature and method. In this study, we investigated saponins and antioxidant contents in dried P. grandiflorum roots either not cut or cut to different lengths. Methods and Results : P. grandiflorum roots were either cut to 0.5, 1.0 or 2.0 cm in length or left uncut. They were dried at $45^{\circ}C$ and the effect of antioxidant substances, antioxidant ability, and saponin content were investigated. P. grandiflorum roots rapidly dried out when cut to short lengths. The saponin contents (platycodin D, polygalacin D, and deapioplatycodin D) was highest in the uncut P. grandiflorum roots. When the cut lengths were long, the levels of polyphenolic compound increased. However, 2,2'-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were higher in the samples cut to shorter lengths. Conclusions : These results suggested that the saponin and antioxidant content of P. grandiflorum roots could be maximized by controlling the drying rate of the roots via adjustments to the root cutting length.

Keywords

References

  1. Ahn KS, Noh EJ, Zhao HL, Jung SH, Kang SS and Kim YS. (2005). Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-kB activation in RAW 264.7 cells. Life Science. 76:2315-2328. https://doi.org/10.1016/j.lfs.2004.10.042
  2. Arslan D and Ozcan MM. (2008). Evaluation of drying methods with respect to drying kinetics, mineral content and colour characteristics of rosemary leaves. Energy Conversion and Management. 49:1258-1264. https://doi.org/10.1016/j.enconman.2007.08.005
  3. Choi CY, Kim JY, Kim YS, Chung YC, Seo JK and Jeong HG. (2001). Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factor alpha from murine macrophages. International Immunopharmacology. 1:1141-1151. https://doi.org/10.1016/S1567-5769(01)00047-9
  4. Choi KH, Nam HH and Choo BK. (2013). Effect of five Korean native Taraxacum on antioxidant activity and nitric oxide production inhibitory activity. Korean Journal of Medicinal Crop Science. 21:191-196. https://doi.org/10.7783/KJMCS.2013.21.3.191
  5. Choi MY, Oh HS and Kim JH. (2006) Changes of physicochemical properties of cultivated Codonopsis lanceolata stored at various storage conditions. Korean Journal of Plant Research. 19:59-67.
  6. Dewanto V, Wu X and Liu RH. (2002). Processed sweet corn has higher antioxidant activity. Journal of Agricultural and Food Chemistry. 50:4959-4964. https://doi.org/10.1021/jf0255937
  7. Duval B and Shetty K. (2001). The stimulation of phenolics and antioxidant activity in pea(Pisum sativam) elicited by genetically transformed anise root extract. Journal of Food Biochemistry. 25:361-377. https://doi.org/10.1111/j.1745-4514.2001.tb00746.x
  8. Ha DC, Lee JW, Do JH, Park CK and Ryu GH. (2004). Drying rate and physicochemical characteristics of dried ginseng root at different temperature. Journal of Food Science and Nutrition. 33:741-746.
  9. Hwang SY, Choi HM and Lim SY. (2013). Total phenolics of dried Platycodon grandiflorum and its effect on growth of human cancer cell lines. Korean Journal of Food Science and Technology. 45:84-89. https://doi.org/10.9721/KJFST.2013.45.1.84
  10. Kim HS, Kim TW, Kim DJ, Lee JS, Kim KK and Choe M. (2013). Antioxidant activities and α-glucosidase inhibitory effect of water extracts from medicinal plants. Korean Journal of Medicinal Crop Science. 21:197-203. https://doi.org/10.7783/KJMCS.2013.21.3.197
  11. Kim SM, Cho YS and Sung SK. (2001). The antioxidant ability and nitrite scavenging ability of plant extracts. Korean Journal of Food Science and Technology. 33:626-632.
  12. Konishi T, Tada A, Shoji J, Kasai R and Tanaka O. (1978). The structures of platycodin A and C, monoacetylated saponins of the roots of Platycodon grandiflorum A. DC. Chemical and Pharmaceutical Bulletin. 26:668-670. https://doi.org/10.1248/cpb.26.668
  13. Korean Food and Drug Administration(KFDA). (2011). Food standards codex. Korean Food and Drug Administration. Seoul, Korea. p.330-374.
  14. Kwon GM, Kim JW and Youn KS. (2013). Effect of different pre-treatments on the physicochemical and antioxidant activities of cold-vacuum dried peaches. Korean Journal of Food Science and Technology. 45:466-472. https://doi.org/10.9721/KJFST.2013.45.4.466
  15. Lee BJ and Cho YS. (2014). Effects of drying temperature on the saponin and free sugar contents of Platycodon grandiflorum radix. Korean Journal of Food Science and Technology. 46:769-772. https://doi.org/10.9721/KJFST.2014.46.6.769
  16. Lee BJ, Jeon SH, Lee SW, Chun HS and Cho YS. (2014a). Effects of drying methods on the saponin and mineral contents of Platycodon grandiflorum radix. Korean Journal of Food Science and Technology. 46:636-640. https://doi.org/10.9721/KJFST.2014.46.5.636
  17. Lee BJ, Jeon SH, Lee SW, Chun HS and Cho YS. (2014b). Soil physicochemistry and saponins content of Platycodon grandiflorum radix cultured from different sites in Gyeongnam province. Korean Journal of Medicinal Crop Science. 22:463-468. https://doi.org/10.7783/KJMCS.2014.22.6.463
  18. Lee HY, Kang RH, Kim YS, Chung SI and Yoon YS. (2010). Platycodin D inhibits adipogenesis of 3T3-L1 cells by modulating kruppel-like factor 2 and peroxisome proliferator-activated receptor gamma. Phytotherapy Research. 24(Supplement 2):161-167. https://doi.org/10.1002/ptr.3054
  19. Lee JH and Kim HJ. (2009). Vacuum drying kinetics of Asian white radish(Raphanus sativus L.) slices. LWT-Food Science Technology. 42:180-186. https://doi.org/10.1016/j.lwt.2008.05.017
  20. Lee SM and Lee JS. (2006). Tocopherol and tocotrienol contents of vegetable oils, margarines, butters, and peanut butters consumed in Korean diet. Food Science and Biotechnology. 15:183-188.
  21. Lee SJ, Shin SR and Yoon KY. (2013). Physicochemical properties of black doraji(Platycodon grandiflorum). Korean Journal of Food Science and Technology. 45:422-427. https://doi.org/10.9721/KJFST.2013.45.4.422
  22. Martin JS and Martin MM. (1983). Tannin assays in ecological studies: Precipitation of ribulose-1,5-bisphosphate carboxylase/ oxygenase by tannic acid, quebracho, and oak foliage extracts. Journal of Chemical Ecology. 9:285-294. https://doi.org/10.1007/BF00988046
  23. Middleton E and Kandaswami C. (1994). Potential health promoting properties of citrus flavonoids. Food Technology. 48:115-119.
  24. Mujumdar AS. (2000). Drying Technology in Agriculture and Food Sciences. In Krokida M and Maroulis Z.(ed.). Quality changes druing drying of food materials. Science Publishers Inc. Enfield. NH, USA. p.61-106.
  25. Nakagawa M and Amano I. (1974). Evaluation method of green tea grade by nitrogen analysis. Journal of Japanese Society of Food Science and Technology. 21:57-63. https://doi.org/10.3136/nskkk1962.21.57
  26. National Institute of Agricultural Science and Technology (NIAST). (2000). Methods of soil and plant analysis. Rural Development Administration. Suwon, Korea. p.103-146.
  27. Park IS, Kang EM and Kim NS. (2000). High-performance liquid chromatographic analysis of saponin compounds in Bupleurum falcatum. Journal of Chromatographic Science. 38:229-233. https://doi.org/10.1093/chromsci/38.6.229
  28. Rice-Evans CA, Miller NJ and Paganga G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science. 2:152-159. https://doi.org/10.1016/S1360-1385(97)01018-2
  29. Sung NJ and Seo JK. (1998). Medical action of perennial Platycodon radix. In proceeding Institute of Agricultural Resource Utilization Symposium for 50th anniversary GSNU. Institute of Agricultural Resource Utilization. Gyeongsang National University. Korea. p.35-47.
  30. Swami SB, Das SK and Maiti B. (2007). Convective hot air drying and quality characteristics of bori: A traditional Indian nugget prepared black gran pulse batter. Journal of Food Engineering. 79:225-233. https://doi.org/10.1016/j.jfoodeng.2006.01.064
  31. Tada T, Kaneiwa Y, Shoji J and Shibat S. (1975). Saponins of the root of Platycodon grandiflorum: Isolation and the structure of platycodin D. Chemical and Pharmaceutical Bulletin. 23:2965-2972. https://doi.org/10.1248/cpb.23.2965
  32. Wang C, Schuller-Levis GB, Lee EB, Levis WR, Lee DW, Kim BS, Park SY and Park E. (2004). Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-$\alpha$ in activated RAW 264.7 cells. International Immunopharmacology. 4:1039-1049. https://doi.org/10.1016/j.intimp.2004.04.005
  33. Zhao HL, Cho KH, Ha YW, Jeong TS, Lee WS and Kim YS. (2006). Cholesterol-lowering effect of platycodin D in hypercholesterolemic ICR mice. European Journal of Pharmacology. 537:166-173. https://doi.org/10.1016/j.ejphar.2006.03.032

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