Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-adipogenic Effects of the Water Extracts of Defatted Green Tea Seed Cake

녹차씨 압착박 열수 추출물의 지방세포 분화 억제 효과

  • 위지향 ((재)전남생물산업진흥원 식품산업연구센터) ;
  • 성혜미 ((재)전남생물산업진흥원 식품산업연구센터) ;
  • 정경옥 ((재)전남생물산업진흥원 식품산업연구센터) ;
  • 김숙정 ((재)전남생물산업진흥원 식품산업연구센터) ;
  • 신유림 ((주)바이오푸드스토리) ;
  • 박주현 ((주)바이오푸드스토리) ;
  • 김종덕 (전남대학교)
  • Received : 2015.05.20
  • Accepted : 2015.06.24
  • Published : 2015.08.31
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Abstract

The effect of the hot water extract of defatted green tea seed cake (GTSE) on lipid metabolism and the underlying mechanisms of lipolysis in mature 3T3-L1 adipocytes were investigated. In this study, we found that the naringenin content of GTSE was 5.5 mg/g; however, catechins were not detected. The intracellular lipid droplets were stained with Oil Red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 52%, and intracellular triglyceride (TG) level was reduced by 33% after treatment with GTSE at a concentration of $40{\mu}g/mL$. To determine the mechanism of reduction in TG content, we determined the level of fatty acid synthase (FAS), phosphorylation of adenosine monophosphate-activated protein kinase (AMPK), and acetyl-coenzyme A carboxylase (ACC) in the cell model. Incubation of the 3T3-L1 adipocytes with GTSE stimulated AMPK and ACC phosphorylation in a dose-dependent manner, and decreased the expression of FAS.

본 연구는 녹차씨 압착박의 활용성을 증가해 보고자 녹차씨를 압착한 후 남은 박을 추출하여 총 페놀, 조사포닌, 카테킨, 나린게닌 함량을 확인하고 3T3-L1 세포에서의 지방세포분화 억제 관련 효과를 확인하였다. 또한 기존에 체지방 감소 효능이 있는 것으로 알려진 녹차와도 비교하였다. 녹차씨 압착박 추출물과 녹차 추출물의 페놀, 조사포닌, 카테킨, 나린게닌 함량을 비교한 결과 녹차씨 압착박 추출물에서 나린게닌 함량이 높음을 알 수 있었다. 3T3-L1 세포에서 Oil Red O 염색법을 이용한 지방 축적저해 정도 및 세포내 중성지방 함량을 측정한 결과, 녹차씨 압착박 추출물에서 지방 축적저해 및 중성지방의 함량이 감소하는 것을 확인하였다. 녹차씨 압착박이 지방합성 및 에너지 조절 효소에 미치는 영향을 알아보기 위해 단백질 발현 실험을 실시한 결과, 녹차 또는 녹차씨 압착박 추출물에 의해서 AMPK 및 ACC의 인산화 증가 및 FAS의 발현이 감소하는 것을 확인 할 수 있었으며, 이러한 효능은 녹차 추출물보다 녹차씨 압착박 추출물에서 더 강하게 나타나는 것을 알 수 있었다. 이상의 결과로부터 녹차씨 압착박 추출물이 녹차 추출물 보다 지방세포분화 억제 효과가 높게 나타나는 것이 확인되어져, 녹차씨는 추후 체지방 감소 효능을 갖는 식품 소재로도 활용이 가능 할 것으로 생각되어진다.

Keywords

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