YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Isolation of Constituents of Lihtospermum erythrorhizon and Development of an Analytical Method of its Constituents by HPLC-UV

자근의 성분 분리 및 이들 성분의 HPLC-UV 동시분석법 개발

  • Received : 2015.09.09
  • Accepted : 2015.10.26
  • Published : 2015.10.31
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Abstract

Lithospermi radix, the dried roots of Litospermum erythrorhizon Sieboid et Zuccarini (Boraginaceae), has long been used to treat detoxification and inflammation. In this study, we isolated two main quinoid compounds, ${\beta}$-hydroxyisovalerylshikonin (1) and acetylshikonin (2) from L. erythrorhizon. As acetylshikonin is considered as a marker compound of L. erythrorhizon, a rapid analysis method for the simultaneous determination of quinoid compounds including 2 was also developed by HPLC (High Performance Liquid Chromatography) and validation of this analytical method. By the developed method, two quinoid marker compounds (${\beta}$-hydroxyisovalerylshikonin and acetylshikonin) were successfully quantified in 31 commercial samples which were collected from different regions. The contents were 0.20% (${\beta}$-hydroxyisovalerylshikonin) and 0.22% (acetylshikonin), respectively.

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References

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