Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Comparison of Pectin Hydrogel Collection Methods in Microfluidic Device

미세유체 장치에서 수거 방법에 따른 펙틴 하이드로겔 입자의 특성 비교

  • Kim, Chaeyeon (Department of Energy Science and Technology, Graduate School of Energy Science and Technology, Chungnam National University) ;
  • Park, Ki-Su (Department of Chemical Engineering, Chungnam National University) ;
  • Kang, Sung-Min (Department of Chemical Engineering, Chungnam National University) ;
  • Kim, Jongmin (Department of Chemical Engineering, Chungnam National University) ;
  • Song, YoungShin (Department of Chemical Engineering, Chungnam National University) ;
  • Lee, Chang-Soo (Department of Chemical Engineering, Chungnam National University)
  • 김채연 (충남대학교 에너지과학기술대학원 에너지과학기술학과) ;
  • 박기수 (충남대학교 공과대학 화학공학과) ;
  • 강성민 (충남대학교 공과대학 화학공학과) ;
  • 김종민 (충남대학교 공과대학 화학공학과) ;
  • 송영신 (충남대학교 공과대학 화학공학과) ;
  • 이창수 (충남대학교 공과대학 화학공학과)
  • Received : 2015.06.19
  • Accepted : 2015.08.21
  • Published : 2015.12.01
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Abstract

This study investigated the effect of different collection methods on physical properties of pectin hydrogels in microfluidic synthetic approach. The pectin hydrogels were simply produced by the incorporation of calcium ions dissolved in continuous mineral oil. Then, different collection methods, pipetting, tubing, and settling, for harvesting pectin hydrogels were applied. The settling method showed most uniform and monodispersed hydrogels. In the case of settling, a coefficient of variation was 3.46 which was lower than pipetting method (18.60) and tubing method (14.76). Under the settling method, we could control the size of hydrogels, ranging from $30{\mu}m$ to $180{\mu}m$, by simple manipulation of the viscosity of pectin and volumetric flow rate of dispersed and continuous phase. Finally, according to the characteristics of simple encapsulation of biological materials, we envision that the pectin hydrogels can be applied to drug delivery, food, and biocompatible materials.

본 연구는 미세유체 장치를 통해 제조가 이루어진 펙틴 하이드로겔 입자의 수거 방법을 다르게 하였을 때 각 방법에 따른 하이드로겔의 물리적 특성을 비교한 것이다. 펙틴 하이드로겔 입자는 미세유체 채널 내에서 미네랄 오일에 분산된 칼슘 이온에 의해 겔화되고 이후 각각 파이펫팅법, 튜브법, 침전법을 통해 수거하였다. 각 방법으로 수거된 펙틴 하이드로겔 입자의 단분산성을 분석한 결과 침전법의 변동 계수(Coefficient of variation)는 3.46으로 파이펫팅법(18.60)과 튜브법(14.76)의 변동 계수보다 월등히 낮아 가장 우수한 단분산성 하이드로겔 입자를 만들 수 있었다. 상기 침전법을 이용한 조건에서 분산상과 연속상의 부피유속 및 펙틴 용액의 점도를 조절함으로써 $30{\mu}m$에서 $180{\mu}m$까지의 다양한 크기를 갖는 단분산성 펙틴 하이드로겔을 제조할 수 있었다. 본 논문에서 제시한 펙틴 하이드로겔 입자는 생체 물질을 손쉽게 함입할 수 있으므로 이는 향후 약물전달, 식품, 그리고 생체적합성 재료 등으로 활용 가능할 것으로 기대된다.

Keywords

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