Korean Chemical Engineering Research

  • 제53권6호
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  • Pages.802-807
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  • 2015
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  • 0304-128X(pISSN)
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  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
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새로운 가교제를 적용한 촉매를 이용한 글루코스 센서의 성능향상 연구

A Study on Performance Improvement of Glucose Sensor Adopting a Catalyst Using New Cross Liker

  • 정용진 (서울과학기술대학교 에너지환경대학원) ;
  • 권용재 (서울과학기술대학교 에너지환경대학원)
  • Chung, Yongjin (Graduate School of Energy and Environment, Seoul National University of Science and Technology) ;
  • Kwon, Yongchai (Graduate School of Energy and Environment, Seoul National University of Science and Technology)
  • 투고 : 2015.08.06
  • 심사 : 2015.09.17
  • 발행 : 2015.12.01
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⟨ 이전 논문 다음 논문 ⟩

초록

본 논문에서는 글루코스산화제, polyethyleneimine(PEI) 및 탄소나노튜브 간 물리적 흡착으로 제조된 촉매(GOx/PEI/CNT)에 새로운 가교제인 terephthalaldehyde(TPA)를 첨가하여 민감도 및 안정성이 개선된 글루코스 센서 촉매를 합성하여, 감지능 및 안정성 개선효과를 확인하였다. 새로운 가교제를 포함한 바이오 촉매는, 글루코스산화제 및 polyethyeleneimine의 관능기와 TPA의 관능기간 알돌축합반응에 의해 생성되었고, 이를 통해 생성된 새로운 전자전달구조는 글루코스의 산화반응을 촉진시켰다. 이러한 촉매활성은 전기화학적 평가를 통해 정량적으로 평가하였으며 그 결과 $41.1{\mu}Acm^{-2}mM^{-1}$의 글루코스 민감도를 얻을 수 있었다. 또한 가교제와 글루코스산화제 및 polyethyeleneimine 간의 화학반응의 형성에 의해 글루코스 산화제의 외부 손실을 최소화 하여, 센서 안정성 향상에도 크게 기여하였다. 안정성 평가를 한 결과, 3주간의 주기적인 촉매 활성 측정후에 94.6% 활성이 유지됨을 확인하였다.

In this study, we synthesized a new biocatalyst consisting of glucose oxidase (GOx), polyethyleneimine (PEI) and carbon nanotube (CNT) with addition of terephthalaldehyde (TPA) (TPA/GOx/PEI/CNT) for fabrication of glucose sensor that shows improved sensing ability and stability compared with that using other biocatalysts. Main bonding of the new TPA/GOx/PEI/CNT catalyst is formed by Aldol condensation reaction of functional end groups between GOx/PEI and TPA. Such formed bonding structure promotes oxidation reaction of glucose. Catalytic activity of TPA/GOx/PEI/CNT is evaluated quantitatively by electrochemical measurements. As a result of that, large sensitivity value of $41{\mu}Acm^{-2}mM^{-1}$ is gained. Regarding biosensor stability of TPA/GOx/PEI/CNT catalyst, covalent bonding formed between GOx/PEI and TPA prevents GOx molecules from becoming leaching-out and contributes improvement in biosensor stability. With estimation of the biosensor stability, it is found that the TPA/GOx/PEI/CNT catalyst keeps 94.6% of its initial activity even after three weeks.

키워드

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피인용 문헌

  1. Highly sensitive glucose biosensor using new glucose oxidase based biocatalyst vol.34, pp.11, 2017, https://doi.org/10.1007/s11814-017-0224-9
  2. Effects of the gold nanoparticles including different thiol functional groups on the performances of glucose-oxidase-based glucose sensing devices pp.1975-7220, 2018, https://doi.org/10.1007/s11814-018-0163-0
  3. 글루코스 기반 바이오연료전지를 위한 다양한 분자량의 폴리에틸렌이민을 이용한 글루코스 산화효소 고정화 vol.54, pp.5, 2016, https://doi.org/10.9713/kcer.2016.54.5.693
  4. 시료용액의 특성에 따른 고정화된 단일벽 탄소나노튜브의 전기적 거동 vol.55, pp.1, 2017, https://doi.org/10.9713/kcer.2017.55.1.115
  5. 백금 나노입자가 분산된 3차원 산화구리 나노구조체 기반의 글루코스 검출용 비효소적 전기화학 센서 개발 vol.56, pp.5, 2015, https://doi.org/10.9713/kcer.2018.56.5.705