Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-Wrinkle Effects of Enzymatic Oyster Hydrolysate and Its Fractions on Human Fibroblasts

굴 효소 가수분해물과 분자량 분획물의 섬유아세포에 대한 주름 개선 효과

  • Kim, Hyeon-Ah (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Park, Si-Hyang (Sunmarine Biotech. Co., Ltd.) ;
  • Lee, Su-Seon (Sunmarine Biotech. Co., Ltd.) ;
  • Choi, Yeung Joon (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
  • 김현아 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 박시향 ((주)선마린바이오테크) ;
  • 이수선 ((주)선마린바이오테크) ;
  • 최영준 (경상대학교 해양식품공학과/해양산업연구소)
  • Received : 2015.07.09
  • Accepted : 2015.08.06
  • Published : 2015.11.30
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Abstract

We investigated the anti-wrinkle efficacy of hydrolysate from oyster protein by Protamex and Neutrase for the purpose of finding materials to assist skin health originating from marine organisms. There were about 7.9% free amino acids in the oyster hydrolysate, and contents of urea, taurine, alanine, and glycine were high. Oyster hydrolysate also showed collagenase inhibitory activity and was not toxic to CCD986sk human fibroblast cells. Yield of the fractions according to the molecular weight of oyster hydrolysate was 40% for less than 1,000 Da and 60.4% for less than 5,000 Da, respectively. Antioxidative effect, procollagen production, and matrix metalloproteinase-1 inhibitory activity were highest in 1,000~3,000 Da fractions. We observed that oyster hydrolysate and its less than 5,000 Da fraction are potential functional compounds for skin health and for improving wrinkles.

해양생물 유래 피부건강 기능성의 소재를 발굴할 목적으로 굴 단백질을 Protamex와 Neutrase로 가수분해 한 후 생성된 굴 가수분해물 펩타이드의 주름 개선 효과를 조사하였다. 굴 가수분해물은 약 7.9%의 유리아미노산을 포함하며 urea, taurine, alanine, glycine 등의 아미노산의 함량이 높았다. 굴 가수분해물은 collagenase 저해 활성을 가지고 있었고, 피부세포인 CCD986sk에 대한 독성은 없었다. 분획물의 수율은 1,000 Da 미만이 40%였으며, 5,000 Da 미만이 60.4%를 차지하였다. 1,000~3,000 Da인 분획물이 항산화 활성, procollagen의 생성능 및 MMP-1 효소 저해 활성이 가장 높았다. 굴 가수분해물과 5,000 Da 미만의 분획물은 주름 개선을 위한 피부건강 기능성 소재로서 화장품 등의 제품에 응용 가능함을 확인하였다.

Keywords

References

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