Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Antiobesity effects of the water-soluble fraction of the ethanol extract of Smilax china L. leaf in 3T3-L1 adipocytes

  • Kang, Yun Hwan (Research and Development Division, Korean Promotion Institute for Traditional Medicine Industry) ;
  • Kim, Kyoung Kon (Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University) ;
  • Kim, Dae Jung (Well-Being Bioproducts R&D Regional Innovation Center, Kangwon National University) ;
  • Choe, Myeon (Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University)
  • Received : 2015.02.27
  • Accepted : 2015.06.16
  • Published : 2015.12.01
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Abstract

BACKGROUND/OBJECTIVES: Several medicinal properties of Smilax china L. have been studied including antioxidant, anti-inflammatory, and anti-cancer effects. However, the antiobesity activity and mechanism by which the water-soluble fraction of this plant mediates its effects are not clear. In the present study, we investigated the lipolytic actions of the water-soluble fraction of Smilax china L. leaf ethanol extract (wsSCLE) in 3T3-L1 adipocytes. MATERIALS/METHODS: The wsSCLE was identified by measuring the total polyphenol and flavonoid content. The wsSCLE was evaluated for its effects on cell viability, lipid accumulation, glycerol, and cyclic adenosine monophosphate (cAMP) contents. In addition, western blot analysis was used to evaluate the effects on protein kinase A (PKA), PKA substrates (PKAs), and hormone-sensitive lipase (HSL). For the lipid accumulation assay, 3T3-L1 adipocytes were treated with different doses of wsSCLE for 9 days starting 2 days post-confluence. In other cell experiments, mature 3T3-L1 adipocytes were treated for 24 h with wsSCLE. RESULTS: Results showed that treatment with wsSCLE at 0.05, 0.1, and 0.25 mg/mL had no effect on cell morphology and viability. Without evidence of toxicity, wsSCLE treatment decreased lipid accumulation compared with the untreated adipocyte controls as shown by the lower absorbance of Oil Red O stain. The wsSCLE significantly induced glycerol release and cAMP production in mature 3T3-L1 cells. Furthermore, protein levels of phosphorylated PKA, PKAs, and HSL significantly increased following wsSCLE treatment. CONCLUSION: These results demonstrate that the potential antiobesity activity of wsSCLE is at least in part due to the stimulation of cAMP-PKA-HSL signaling. In addition, the wsSCLE-stimulated lipolysis induced by the signaling is mediated via activation of the ${\beta}$-adrenergic receptor.

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References

  1. McMorrow AM, Connaughton RM, Lithander FE, Roche HM. Adipose tissue dysregulation and metabolic consequences in childhood and adolescent obesity: potential impact of dietary fat quality. Proc Nutr Soc 2015;74:67-82. https://doi.org/10.1017/S002966511400158X
  2. Kopelman PG. Obesity as a medical problem. Nature 2000;404:635-43. https://doi.org/10.1038/35007508
  3. Okabe Y, Shimada T, Horikawa T, Kinoshita K, Koyama K, Ichinose K, Aburada M, Takahashi K. Suppression of adipocyte hypertrophy by polymethoxyflavonoids isolated from Kaempferia parviflora. Phytomedicine 2014;21:800-6. https://doi.org/10.1016/j.phymed.2014.01.014
  4. Chaves VE, Frasson D, Kawashita NH. Several agents and pathways regulate lipolysis in adipocytes. Biochimie 2011;93:1631-40. https://doi.org/10.1016/j.biochi.2011.05.018
  5. Holm C. Molecular mechanisms regulating hormone-sensitive lipase and lipolysis. Biochem Soc Trans 2003;31:1120-4. https://doi.org/10.1042/bst0311120
  6. Okumura T, Harada K, Oue K, Zhang J, Asano S, Hayashiuchi M, Mizokami A, Tanaka H, Irifune M, Kamata N, Hirata M, Kanematsu T. Phospholipase C-related catalytically inactive protein (PRIP) regulates lipolysis in adipose tissue by modulating the phosphorylation of hormone-sensitive lipase. PLoS One 2014;9:e100559. https://doi.org/10.1371/journal.pone.0100559
  7. Kim SO, Sakchaisri K, Asami Y, Ryoo IJ, Choo SJ, Yoo ID, Soung NK, Kim YS, Jang JH, Kim BY, Ahn JS. Illudins C2 and C3 stimulate lipolysis in 3T3-L1 adipocytes and suppress adipogenesis in 3T3-L1 preadipocytes. J Nat Prod 2014;77:744-50. https://doi.org/10.1021/np400520a
  8. Jaworski K, Sarkadi-Nagy E, Duncan RE, Ahmadian M, Sul HS. Regulation of triglyceride metabolism. IV. Hormonal regulation of lipolysis in adipose tissue. Am J Physiol Gastrointest Liver Physiol 2007;293:G1-4. https://doi.org/10.1152/ajpgi.00554.2006
  9. Chaput JP, St-Pierre S, Tremblay A. Currently available drugs for the treatment of obesity: Sibutramine and orlistat. Mini Rev Med Chem 2007;7:3-10. https://doi.org/10.2174/138955707779317849
  10. Collins P, Williams G. Drug treatment of obesity: from past failures to future successes? Br J Clin Pharmacol 2001;51:13-25.
  11. Li M, Cheung BM. Pharmacotherapy for obesity. Br J Clin Pharmacol 2009;68:804-10. https://doi.org/10.1111/j.1365-2125.2009.03453.x
  12. Vickers SP, Cheetham SC, Headland KR, Dickinson K, Grempler R, Mayoux E, Mark M, Klein T. Combination of the sodium-glucose cotransporter-2 inhibitor empagliflozin with orlistat or sibutramine further improves the body-weight reduction and glucose homeostasis of obese rats fed a cafeteria diet. Diabetes Metab Syndr Obes 2014;7:265-75.
  13. Kumar P, Bhandari U, Jamadagni S. Fenugreek seed extract inhibit fat accumulation and ameliorates dyslipidemia in high fat diet-induced obese rats. Biomed Res Int 2014;2014:606021.
  14. Cha BC, Lee EH. Antioxidant activities of flavonoids from the leaves of Smilax china linne. Korean J Pharmacogn 2007;38:31-6.
  15. Kim SH, Ahn JH, Jeong JY, Kim SB, Jo YH, Hwang BY, Lee MK. Tyrosinase inhibitory phenolic constituents of Smilax china leaves. Korean J Pharmacogn 2013;44:220-3.
  16. Aguirre L, Fernandez-Quintela A, Arias N, Portillo MP. Resveratrol: anti-obesity mechanisms of action. Molecules 2014;19:18632-55. https://doi.org/10.3390/molecules191118632
  17. Park UH, Jeong JC, Jang JS, Sung MR, Youn H, Lee SJ, Kim EJ, Um SJ. Negative regulation of adipogenesis by kaempferol, a component of Rhizoma Polygonati falcatum in 3T3-L1 cells. Biol Pharm Bull 2012;35:1525-33. https://doi.org/10.1248/bpb.b12-00254
  18. Wang S, Moustaid-Moussa N, Chen L, Mo H, Shastri A, Su R, Bapat P, Kwun I, Shen CL. Novel insights of dietary polyphenols and obesity. J Nutr Biochem 2014;25:1-18. https://doi.org/10.1016/j.jnutbio.2013.09.001
  19. Sakagami Y. Inhibitory effect of the extract of Prunus mume Sieb. et Zucc. on vero-toxin production by enterohemorrhagic Escherichia coli O157 : H7. Biocontrol Sci 2001;6:53-6. https://doi.org/10.4265/bio.6.53
  20. Kim KK, Kang YH, Kim DJ, Kim TW, Choe M. Comparison of antioxidant, $\alpha$-glucosidase inhibition and anti-inflammatory activities of the leaf and root extracts of Smilax china L. J Nutr Health 2013;46:315-23. https://doi.org/10.4163/jnh.2013.46.4.315
  21. Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 2000;71:109-14. https://doi.org/10.1016/S0378-8741(99)00189-0
  22. Yadav D, Chaudhary AA, Garg V, Anwar MF, Rahman MM, Jamil SS, Khan HA, Asif M. In vitro toxicity and antidiabetic activity of a newly developed polyherbal formulation (MAC-ST/001) in streptozotocin-induced diabetic Wistar rats. Protoplasma 2013;250:741-9. https://doi.org/10.1007/s00709-012-0458-7
  23. Oh SD, Kim M, Min BI, Choi GS, Kim SK, Bae H, Kang C, Kim DG, Park BJ, Kim CK. Effect of Achyranthes bidentata Blume on 3T3-L1 adipogenesis and rats fed with a high-fat diet. Evid Based Complement Alternat Med 2014;2014:158018.
  24. Jin TY, Park JR, Kim JH. Electron donating abilities, nitrite scavenging effects and antimicrobial activities of Smilax china leaf. J Korean Soc Food Sci Nutr 2004;33:621-5. https://doi.org/10.3746/jkfn.2004.33.4.621
  25. Lee SY, Kim JH, Park JM, Lee IC, Lee JY. Antioxidant activity and inhibition activity against $\alpha$-amylase and $\alpha$-glucosidase of Smilax china L. Korean J Food Preserv 2014;21:254-63. https://doi.org/10.11002/kjfp.2014.21.2.254
  26. Park HS, Kim GH, Shim SM. Different effect of methanol extracts and bioaccessible fraction of Smilax china on triglyceride accumulation in adipocytes. J Food Biochem 2014;38:1-5. https://doi.org/10.1111/jfbc.12018
  27. Brasaemle DL, Levin DM, Adler-Wailes DC, Londos C. The lipolytic stimulation of 3T3-L1 adipocytes promotes the translocation of hormone-sensitive lipase to the surfaces of lipid storage droplets. Biochim Biophys Acta 2000;1483:251-62. https://doi.org/10.1016/S1388-1981(99)00179-1
  28. Egan JJ, Greenberg AS, Chang MK, Londos C. Control of endogenous phosphorylation of the major cAMP-dependent protein kinase substrate in adipocytes by insulin and beta-adrenergic stimulation. J Biol Chem 1990;265:18769-75.
  29. Guellich A, Mehel H, Fischmeister R. Cyclic AMP synthesis and hydrolysis in the normal and failing heart. Pflugers Arch 2014;466:1163-75. https://doi.org/10.1007/s00424-014-1515-1

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