Korean Journal of Materials Research (한국재료학회지)

Materials Research Society of Korea (한국재료학회)
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Characterization of Graphene Oxide Suspension for Fluorescence Quenching in DNA-Diagnostics

  • Kapitonov, A.N. (M.K. Ammosov North-Eastern Federal University) ;
  • Alexandrov, G.N. (M.K. Ammosov North-Eastern Federal University) ;
  • Vasileva, F.D. (M.K. Ammosov North-Eastern Federal University) ;
  • Smagulova, S.A. (M.K. Ammosov North-Eastern Federal University) ;
  • Timofeev, V.B. (M.K. Ammosov North-Eastern Federal University) ;
  • Maksimova, N.R. (M.K. Ammosov North-Eastern Federal University) ;
  • Kuznetsov, A.A. (M.K. Ammosov North-Eastern Federal University)
  • Received : 2015.09.07
  • Accepted : 2015.11.09
  • Published : 2016.01.27
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Abstract

The graphene oxides (GOs) were tested as a fluorescent quencher in the field of DNA-diagnostics. The various suspensions of GO nanoplates were prepared by changing the synthesis conditions. The suspensions were stable for at least 6 weeks by differing degrees of functionalization of various oxygen-containing groups of atoms. Depending on the properties of GO nanoplates, their fluorescent quenching abilities, which were determined by the amount of the tagged immobilized oligonucleotide, were also changed. GO suspension synthesized at $75^{\circ}C$ of reaction mixture showed the fluorescent quenching of 16.39 nmol/mg, which would be a potential substitution of molecular fluorescent quencher in test-systems for DNA-diagnostics.

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