Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Probiotic Effects of Lactobacillus plantarum and Leuconostoc mesenteroides Isolated from Kimchi

김치에서 분리한 Lactobacillus plantarum과 Leuconostoc mesenteroides의 프로바이오틱 효과

  • Lee, Kyung-Hee (Department of Food Science and Nutrition, Pusan National University) ;
  • Bong, Yeon-Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Lee, Hyun Ah (Kimchi Research Institute, Pusan National University) ;
  • Kim, Hee-Young (Department of Food Science and Nutrition, Pusan National University) ;
  • Park, Kun-Young (Department of Food Science and Nutrition, Pusan National University)
  • Received : 2015.09.21
  • Accepted : 2015.11.02
  • Published : 2016.01.31
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Abstract

The probiotic effects of kimchi lactic acid bacteria (LAB), Lactobacillus plantarum (Lab. plantarum) and Leuconostoc mesenteroides (Leu. mesenteroides), were studied. Lab. plantarum KCCM 11352P (LPpnu) and Leu. mesenteroides KCCM 11353P (LMpnu) were isolated from kimchi and were the predominant LAB. We compared their probiotic effects with Lactobacillus rhamnosus GG (LRgg), a well-known probiotic LAB. LPpnu showed better probiotic activities than LRgg. LMpnu also exhibited almost equal activities as LRgg. These two kimchi LAB strains exhibited resistance to gastric and bile acid, adhesion to intestines, and thermal stability. In particular, LPpnu showed excellent probiotic properties. In addition, LPpnu showed greater antioxidant activity by scavenging DPPH radicals or hydroxyl radicals than LMpnu or LRgg. LPpnu also inhibited growth of HT-29 human colon cancer cells by inducing apoptosis, increasing Bax and suppressing Bcl-2 expression compared to LMpnu or LRgg. Taken together, LPpnu and LMpnu could be used as probiotics, and LPpnu exhibited the most beneficial probiotic activities with anti-oxidant and anti-cancer properties.

본 연구에서는 김치에서 분리한 우점유산균인 Lactobacillus plantarum KCCM 11352P(LPpnu)와 Leuconostoc mesenteroides KCCM 11353P(LMpnu)의 프로바이오틱 효과를 프로바이오틱 효과가 높기로 잘 알려진 Lactobacillus rhamnosus GG(LRgg)의 효능과 비교하여 확인하였다. 그 결과 LPpnu는 Lab. rhamnosus GG(LRgg)보다 프로바이오틱 효과가 더 뛰어났으며, LMpnu 또한 LRgg와 거의 유사한 수준의 효능을 나타냈다. LPpnu와 LMpnu는 모두 내산, 내담즙성, 장 부착능, 열 안정성의 프로바이오틱의 기본 특성을 가지고 있었으며, 특히 LPpnu는 가장 높은 프로바이오틱 효과를 나타냈다. 또한 LMpnu 및 LRgg와 비교했을 때 LPpnu는 DPPH radical과 hydroxyl radical 소거능 측정에서 더 높은 항산화능을 보였고, Bcl-2 유전자 발현억제와 Bax 유전자 발현 증가를 통해 apoptosis를 유도함으로써 HT-29 human colon cancer cell에서 높은 암세포 성장 저해 효과를 나타냈다. 이를 통해 김치유래 LPpnu와 LMpnu는 프로바이오틱으로서 이용 가능성이 충분하다고 판단되며, 특히 LPpnu는 항산화 및 항암 활성에 뛰어난 효능을 나타내었기에 중요한 프로바이오틱 균주라고 하겠다.

Keywords

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