Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Effects of Gami-Handayeolso-Tang on Body Fat Reduction in High Fat Diet-Fed Obese Mice

가미한다열소탕(加味寒多熱少湯)이 고지방식이 비만생쥐의 체지방감소에 미치는 영향

  • Lee, Ha-Il (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-Kwang University) ;
  • Lee, Jong-Ha (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-Kwang University) ;
  • Kwon, Young-Mi (Department of Radiology, College of Korean Meidicine, Won-Kwang University) ;
  • Song, Yung-Sun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-Kwang University)
  • 이하일 (원광대학교 한의과대학 한방재활의학교실) ;
  • 이종하 (원광대학교 한의과대학 한방재활의학교실) ;
  • 권영미 (원광대학교 한의과대학 영상의학과) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학교실)
  • Received : 2016.01.03
  • Accepted : 2016.01.22
  • Published : 2016.01.31
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Abstract

Objectives In this study, it was investigated whether Gami-Handayeolso-Tang (HDYST) medication has anti-obesity effects in high fat diet (HFD)-fed obese mice. Methods The experimental animals were divided into five groups-normal diet-fed (ND), high fat diet-fed control (HFD), HFD+HDYST 150, HFD+HDYST 300, and HFD+orlistat as a positive drug. The obese markers such as body weight, diet efficiency ratio, serum levels of total cholesterol, triglyceride, lipid contents, leptin, adiponectin, and GOT/GPT were measured. Also, white adipose tissue, liver weight, abdominal fat mass, hepatic lipid contents, and mRNA expression of obese-associating genes were examined in obese mice. Results In high fat diet-fed mice, HDYST administration significantly decreased body weight, diet efficiency ratio, serum levels of total cholesterol, triglyceride, LDL-cholesterol, as well as leptin and GOT/GPT, compared to the HFD group in a dose-dependent manner. HDYST increased significantly the serum levels of HDL-cholesterol and adiponectin. It also reduced the accumulation of lipids, such as total lipid and triglycerides, in organs such as liver and abdominal adipose tissue. Moreover, HDYST administration significantly decreased the expression levels of fatty acid synthetic genes, such as sterol regulatory element-binding protein-1c (SREBP-1c), FAS and Stearoyl-Coenzyme A desaturase 1 (SCD-1), in the liver tissues, while it increased the messenger RAN (mRNA) levels of fatty acid catalytic genes, such as Peroxisome proliferator activated receptor alpha (PPAR-${\alpha}$), acyl-COA oxidase (ACO), and Carnitine palmitoyltransferase-1a (CPT-1a). Conclusions Based on the results above, HDYST reveals anti-obesity effects declining body fat accumulation through the regulation of fatty acid metabolism and leptin/adiponectin serum levels. It therefore suggests that HDYST can be clinically useful for the treatment of obesity.

Keywords

References

  1. Ahima RS, Flier JS. Adipose tissue as an endocrine organ. Trends Endocrinol Metab. 2000;11(8):327-32. https://doi.org/10.1016/S1043-2760(00)00301-5
  2. Bray GA, Tartaglia LA. Medicinal strategies in the treatment of obesity. Nature. 2000;404(6778):672-7. https://doi.org/10.1038/35007544
  3. Hwang MJ, Shin HD, Song MY. Review of literature on herbal medicines for the treatment of obesity in Korea : mainly papers since 2000. J Korean Med Rehab. 2006; 16(3):65-81.
  4. Hwang MJ, Shin HD, Song MY. Literature Review of Herbal Medicines on Treatment of Obesity Since 2000 -Mainly about Ephedra Herba. J Korean Med Obes Res. 2007;7(1):39-54.
  5. Lee YJ, Lee MJ. The efficiency of Ephedrae herba on the obese zucker rats' lipid of blood serum and fatty acid metabolism of skeletal muscles. J Oriental Rehab Med. 2005;15(3):99-111.
  6. Song YB. Gejeongjungbo Sasangeuihak. Seoul:Jipmoondang. 2008:201, 421, 705-8.
  7. Kim JW, Shin SW, Lee YS, Lee GH, Park JH. Anti-Allergic Effect of Handayeolso-Tang. Korean J Oriental Physiology & Pathology. 2008;22(4):307-14.
  8. Park SS. An Experimental Studies on the Efficacy of Taeumin Handayolsotang and Gamihandayolsotiang. J Const Med. 1992;4(1):231-40.
  9. Kim JJ, Lim HH. Effects of Hadayulso-tang for Taeum-in on the Body Weigth and Lipid Metabolism of Induced Obesity Rats. J Korean Med Obes Res. 2002;2(1):95-104.
  10. Folch J, Lees M, Sloane-Stanley G. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957;226(1): 497-509.
  11. Frings CS, Dunn RT. A colorimetric method for determination of total serum lipids based on the sulfo-phosphovanillin reaction. Am J Clin Pathol. 1970;53(1): 89-91. https://doi.org/10.1093/ajcp/53.1.89
  12. Yang BG. 2013 Health behaviour and chronic disease stats. Korea Centers for Disease Control and Prevention. 2014;12:22-3.
  13. Yoon NH, Kwon SM. Impact of Obesity on Health Care Utilization and Expenditure. The Korean Journal of Health Economics and Policy. 2013;19(2):61-80.
  14. Moriarty JP, Branda ME, Olsen KD, Shah ND, Borah BJ, Wagie AE, Egginton JS, Naessens JM. The effects of incremental costs of smoking and obesity on health care costs among adults: a 7-year longitudinal study. J Occup Environ Med. 2012;54(3):286-91. https://doi.org/10.1097/JOM.0b013e318246f1f4
  15. Lefebvre PJ, Scheen AJ. Obesity: Causes and new treatments. Exp. Clin. Endocrinol Diabetes. 2001:109(Suppl 2):S215-S224. https://doi.org/10.1055/s-2001-18583
  16. Lee M, Aronne LJ. Weight management for type 2 diabetes mellitus: global cardiovascular risk reduction. Am J Cardiol. 2007;99(4A):68B-79B.
  17. Kim KK. Safety of Anti-Obesity Drugs Approved for Long-Term Use. Korean J Obes. 2015;24(1):17-27. https://doi.org/10.7570/kjo.2015.24.1.17
  18. Curfman GD, Morrissey S, Drazen JM. Sibutramine -another flawed diet pill. N Engl J Med. 2010;363(10): 972-4. https://doi.org/10.1056/NEJMe1007993
  19. Gong M, Wang X, Mao Z, Shao Q, Xiang X, Xu B. Effect of electroacupuncture on leptin resistance in rats with diet-induced obesity. Am J Chin Med. 2012;40(3): 511-20. https://doi.org/10.1142/S0192415X12500395
  20. Hsieh CH, Su TJ, Fang YW, Chou PH. Chou. Efficacy of two different materials used in auricular acupressure on weight reduction and abdominal obesity. Am J Chin Med. 2012;40(4):713-20. https://doi.org/10.1142/S0192415X1250053X
  21. Ohnishi R, Matsui-Yuasa I, Deguchi Y, Yaku K, Tabuchi M, Munakata H, Akahoshi Y, Kojima-Yuasa A. 1-acetoxychavicol acetate inhibits adipogenesis in 3T3-L1 adipocytes and in high fat-fed rats. Am J Chin Med. 2012;40(6):1189-204. https://doi.org/10.1142/S0192415X12500887
  22. Wang Q, Li WH, Zhou QH, Tang XD, Zhang XX, Shu S. Weight reduction effects of acupuncture for obese women with or without perimenopausal syndrome: A pilot observa- tional study. Am J Chin Med. 2012;40(6):1157-66. https://doi.org/10.1142/S0192415X12500851
  23. Zhou Q, Chang B, Chen XY, Zhou SP, Zhen Z, Zhang LL, Sun X, Zhou Y, Xie WQ, Liu HF, Xu Y, Kong Y, Zhou LB, Lian FM, Tong XL. Chinese Herbal Medicine for Obesity: A Randomized, Double-Blinded, Multicenter, Prospective Trial. Am J Chin Med. 2014;42(6):1345-56. https://doi.org/10.1142/S0192415X14500840
  24. Moon SH, Sin SH, Kim HJ, Kim JY. A relationshipof the obesity and body composition analysisby Sasang constitution. J Oriental Rehab Med. 2002;12(4):1-10.
  25. Shin SW, Lee Jh. Study on the Characteristics of Ordinary Symptoms in Overweight. and Obesity Patients according to Sasang Constitution. J Korean Med Obes Res. 2013;13(1):33-45.
  26. Kim SO, Yun SJ, Lee EH. The Water Extract of Adlay Seed(Coix lachrymajobi var. mayuen) Exhibits Anti-Obesity Effects Through Neuroendocrine Modulation. Am J Chin Med. 2007;35(2):297-308. https://doi.org/10.1142/S0192415X07004825
  27. Cho YJ. Coix lacryma-jobi L. var. ma-yuen Stapf reduces weight gain in high-fat diet-induced C57BL/6J mice by decreasing expression of C/EBP${\alpha}$ and PPAR${\gamma}$ 2. Kyunghee University. Seoul, Korea. 2013.
  28. Kim JY. The Effects of Raphani Semen on Experimental Mouse Model of Obese diabetes. Dongshin University. Naju, Korea. 2012.
  29. Ding L, Li P, Lau CB, Chan YW, Xu D, Fung KP, Su W. Mechanistic Studies on the Antidiabetic Activity of a Polysaccharide-rich Extract of Radix Ophiopogonis. Phytother Res. 2012;26(1):101-5. https://doi.org/10.1002/ptr.3505
  30. Lee CE, Hur HJ, Hwang JT, Sung MJ, Yang HJ, Kim HJ, Park JH, Kwon DY, Kim MS. Long-term consumption of Platycodi radix ameliorates obesity and Insulin Resistance via the Activation of AMPK Pathways. Evid Based Complement Alternat Med. 2012;2012:759143.
  31. Song KH, Lee SH, Kim BY, Park AY, Kim JY. Extracts of Scutellaria baicalensis Reduced Body Weight and Blood Triglyceride in db/db Mice. Phytother Res. 2013;27(2): 244-50. https://doi.org/10.1002/ptr.4691
  32. Song MY, Kim HJ. Analysis of Main Single Medicinal Herbs for Anti-Obesity. J Korean Med Obes Res. 2013;13(2):51-7.
  33. Hariri N, Thibault L. High-fat diet-induced obesity in animal models. Nutrition Research Reviews. 2010;23(2):270-99. https://doi.org/10.1017/S0954422410000168
  34. Tomkin GH. Atherosclerosis, diabetes and lipoproteins. Expert Rev Cardiovasc Ther. 2010;8(7):1015-29. https://doi.org/10.1586/erc.10.45
  35. Lee JJ, Park MR, Kim AR, Lee MY. Effects of ramie leaves on improvement of lipid metabolism and anti-obesity effect in rats red a high fat/high cholesterol diet. Korean J Food Sci Technol. 2011;43(1):83-90. https://doi.org/10.9721/KJFST.2011.43.1.083
  36. Jeong WG. Food and nutrition dictionary book. Korea Dictionary Research Publishing. 1997:896.
  37. Saely CH, Geiger K, Drexel H. Brown versus White Adipose Tissue: A Mini-Review. Gerontology. 2012;58(1): 15-23. https://doi.org/10.1159/000321319
  38. Satoh N, Naruse M, Usui T, Tagami T, Suganami T, Yamada K, Kuzuya H, Shimatsu A, Ogawa Y. Leptin-toadiponectin ratio as a potential atherogenic index in obese type 2 diabetic patients. Diabetes Care 2004; 27(10):2488-90. https://doi.org/10.2337/diacare.27.10.2488
  39. Szczygielska A1, Widomska S, Jaraszkiewicz M, Knera P, Muc K. Blood lipids profile in obese or overweight patients. Ann Univ Mariae Curie Sklodowska Med. 2003; 58(2):343-9.
  40. Weyer C, Funahashi T, Tanaka S, Hotta K, Matsuzawa Y, Pratley RE, Tataranni PA. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin Endocrinol Metab. 2001;86(5):1930-5. https://doi.org/10.1210/jcem.86.5.7463
  41. Arita Y, Kihara S, Ouchi N, Takahashi M, Maeda K, Miyagawa J, Hotta K, Shimomura I, Nakamura T, Miyaoka K, Kuriyama H, Nishida M, Yamashita S, Okubo K, Matsubara K, Muraguchi M, Ohmoto Y, Funahashi T, Matsuzawa Y. Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun. 1999;257(1):79-83. https://doi.org/10.1006/bbrc.1999.0255
  42. Auwerx J, Staels B. Letpin. THE LANCET. 1998;351: 737-42. https://doi.org/10.1016/S0140-6736(97)06348-4
  43. Tartaglia L. The leptin receptor. J Biol Chem. 1997;272: 6093-6. https://doi.org/10.1074/jbc.272.10.6093
  44. Masuzaki H, Ogawa Y, Sagawa N, Hosoda K, Matsumoto T, Mise H, Nishimura H, Yoshimasa Y, Tanaka I, Mori T, Nakao K. Nonadipose tissue production of leptin: leptin as a novel placenta-derived hormone in humans. Nat Med. 1997;3(9):1029-33. https://doi.org/10.1038/nm0997-1029
  45. van Aggel-Leijssen DP, van Baak MA, Tenenbaum R, Campfield LA, Saris WH. Regulation of average 24h human plasma leptin level; the influence of exercise and physiological changes in energy balance. Int J of Obesity. 1999;23(2):151-58. https://doi.org/10.1038/sj.ijo.0800784
  46. Postic C, Girard J. Contribution of de novo fatty acid synthesis to hepatic steatosis and insulin resistance: lessons from genetically engineered mice. J Clin Invest. 2008;118(3):829-38. https://doi.org/10.1172/JCI34275
  47. Lapsys NM, Kriketos AD, Lim-Fraser M, Poynten AM, Lowy A, Furler SM, Chisholm DJ, Cooney GJ. Expression of genes involved in lipid metabolism correlate with peroxisome proliferator-activated receptor gamma expression in human skeletal muscle. Metab. 2000;85(11):4293-7.
  48. Jogl G, Hsiao YS, Tong L. "Structure and function of carnitine acyltransferases". Annals of the New York Academy of Sciences. 2000);1033(1):17-29. https://doi.org/10.1196/annals.1320.002