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자하거 약침의 지표물질 선정 및 분석법 벨리데이션

Identification of Standard Compound of Ja-ha-guh Pharmacopuncture and Validation of Analytic Methods

  • 박상원 (자생한방병원 한방재활의학과) ;
  • 이재웅 (자생의료재단 척추관절연구소) ;
  • 이진호 (자생한방병원 한방재활의학과) ;
  • 하인혁 (자생한방병원 한방재활의학과) ;
  • 변장훈 (자생한방병원 한방재활의학과) ;
  • 정범환 (자생한방병원 한방재활의학과) ;
  • 정화진 (자생의료재단 척추관절연구소) ;
  • 이인희 (자생의료재단 척추관절연구소) ;
  • 김민정 (자생의료재단 척추관절연구소) ;
  • 김은지 (자생의료재단 척추관절연구소)
  • Park, Sang-won (Department of Korean Rehabilitation Medicine, Ja-Seng Korean Medicine Hospital) ;
  • Lee, Jae-woong (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Lee, Jin-ho (Department of Korean Rehabilitation Medicine, Ja-Seng Korean Medicine Hospital) ;
  • Ha, In-Hyuk (Department of Korean Rehabilitation Medicine, Ja-Seng Korean Medicine Hospital) ;
  • Byun, Jang-hoon (Department of Korean Rehabilitation Medicine, Ja-Seng Korean Medicine Hospital) ;
  • Jung, Bum-hwan (Department of Korean Rehabilitation Medicine, Ja-Seng Korean Medicine Hospital) ;
  • Jung, Hwa-jin (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Lee, In-hee (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Kim, Min-Jeong (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Kim, Eun-Jee (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation)
  • 투고 : 2015.11.30
  • 심사 : 2015.12.10
  • 발행 : 2016.01.31

초록

Objectives Despite the increasing use of Ja-ha-guh (Hominis-placenta ) pharmacopuncture in rehabilitative medicine field, its standard compound has yet to be investigated. The purpose of this study is to provide standardization for future studies and increase satisfaction of patients by utilizing standard pharmacopuncture. Methods Alanine and leucine were selected as potential standard compounds. LC/MS was used to devise an analytic method. This analytic method was subject to validation. According to validation guideline of Korea Food and Drug Administration, the specificity, linearity, precision, range, quantitative limits, detection limits and accuracy were measured. With this analysis, 3 lots of Ja-ha-guh pharmacopuncture were analyzed. Results Because the specificity, linearity, precision, range, quantitative limits, detection limits and accuracy meet criteria of the guideline, the analytic method was validated. It was found that Ja-ha-guh pharmacopuncture contained $211.02{\pm}7.28ug/ml$ of alanine and $372.03{\pm}7.58ug/ml$ of leucine. Conclusions Both alanine and leucine appear to be suitable standard compounds. These results are likely to contribute to further standardization of Ja-ha-guh for medical use.

키워드

참고문헌

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피인용 문헌

  1. Establishment and Selection of Indicator Materials for Cervi Parvum Cornu Pharmacopuncture vol.34, pp.4, 2016, https://doi.org/10.13045/jar.2017.02180
  2. Systematic Review of Hominis Placenta Pharmacopuncture in English and Korean Literature vol.34, pp.4, 2017, https://doi.org/10.13045/jar.2017.02236