대한의용생체공학회:의공학회지 (Journal of Biomedical Engineering Research)

  • 제37권5호
  • /
  • Pages.186-194
  • /
  • 2016
  • /
  • 1229-0807(pISSN)
  • /
  • 2288-9396(eISSN)
대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
권호 표지
DOI QR코드

DOI QR Code

동심축류가 유도되는 미세유체 소자 기반 Collagen Type I 미세섬유의 제작

Fabrication of Collagen Type I Microfiber based on Co-axial Flow-induced Microfluidic Chip

  • 이수경 (강원대학교 공과대학 신소재공학과) ;
  • 이광호 (강원대학교 공과대학 신소재공학과)
  • Lee, Su Kyoung (Department of Advanced Materials Science and Engineering, College of Engineering, Kangwon National University) ;
  • Lee, Kwang-Ho (Department of Advanced Materials Science and Engineering, College of Engineering, Kangwon National University)
  • 투고 : 2016.09.30
  • 심사 : 2016.11.13
  • 발행 : 2016.10.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, a co-axial flow induced microfluidic chip to fabricate pure collagen type I microfiber via the control of collagen type I and Na-alginate gelation process. The pure collagen type I microfiber was generated by selective degradation of Ca-alginate from 'Core-Shell' structured hydrogel microfiber. To make 'Core-Shell' structure, collagen type I solution was introduced into core channel and 1.5% Na-alginate solution was injected into side channel in microfluidic chip. To evaluatethe 'Core-Shell' structure, the red and green fluorescence substances were mixed into collagen type I and Na-alginate solution, respectively. The fluorescence substances were uniformly loaded into each fiber, and the different fluorescence images were dependent on their location. By immoblizing EpH4-Ras and C6 cells within collagen type I and Na-alginate solution, we sucessfully demonstrated the co-culture of EpH4-Ras and C6 cells with 'Core-Shell' like hydrogel microfiber for 5 days. Only to produce pure collagen type I hydrogel fiber, tri-sodium citrate solution was used to dissolve the shell-like Ca-alginate hydrogel fiber from 'Core-Shell' structured hydrogel microfiber, which is an excellent advantage when the fiber is employed in three-dimensional scaffold. This novel method could apply various application in tissue engineering and biomedical engineering.

키워드

참고문헌

  1. Q.L. Loh and C. Choong, "Three-Dimensional Scaffolds for Tissue Engineering Applications: Role of Porosity and Pore Size", Tissue Engineering Part B-Reviews, vol. 19, pp. 485-502, Dec 1 2013. https://doi.org/10.1089/ten.teb.2012.0437
  2. P. Eiselt, J. Yeh, R.K. Latvala, L.D. Shea, and D.J. Mooney, "Porous carriers for biomedical applications based on alginate hydrogels", Biomaterials, vol. 21, pp. 1921-7, Oct 2000. https://doi.org/10.1016/S0142-9612(00)00033-8
  3. E. Carletti, A. Motta, and C. Migliaresi, "Scaffolds for tissue engineering and 3D cell culture", Methods Mol Biol, vol. 695, pp. 17-39, 2011. https://doi.org/10.1007/978-1-60761-984-0_2
  4. F.J. O'Brien, "Biomaterials & scaffolds for tissue engineering", Materials Today, vol. 14, pp. 88-95, Mar 2011. https://doi.org/10.1016/S1369-7021(11)70058-X
  5. R. Langer, "Biomaterials in drug delivery and tissue engineering: one laboratory's experience", Acc Chem Res, vol. 33, pp. 94-101, Feb 2000. https://doi.org/10.1021/ar9800993
  6. R. Vasita and D.S. Katti, "Nanofibers and their applications in tissue engineering", International Journal of Nanomedicine, vol. 1, pp. 15-30, 2006. https://doi.org/10.2147/nano.2006.1.1.15
  7. Y.Z. Zhang, C.T. Lim, S. Ramakrishna, and Z.M. Huang, "Recent development of polymer nanofibers for biomedical and biotechnological applications", Journal of Materials Science-Materials in Medicine, vol. 16, pp. 933-946, Oct 2005. https://doi.org/10.1007/s10856-005-4428-x
  8. S.V. Murphy and A. Atala, "3D bioprinting of tissues and organs", Nature Biotechnology, vol. 32, pp. 773-785, Aug 2014. https://doi.org/10.1038/nbt.2958
  9. H.W. Kang, S.J. Lee, I.K. Ko, C. Kengla, J.J. Yoo, and A. Atala, "A 3D bioprinting system to produce human-scale tissue constructs with structural integrity", Nature Biotechnology, vol. 34, pp. 312-+, Mar 2016. https://doi.org/10.1038/nbt.3413
  10. S. Khetan and J.A. Burdick, "Patterning hydrogels in three dimensions towards controlling cellular interactions", Soft Matter, vol. 7, pp. 830-838, 2011. https://doi.org/10.1039/C0SM00852D
  11. K.C. Hribar, K. Meggs, J. Liu, W. Zhu, X. Qu, and S.C. Chen, "Three-dimensional direct cell patterning in collagen hydrogels with near-infrared femtosecond laser", Scientific Reports, vol. 5, Nov 25 2015.
  12. K.H. Lee, S.J. Shin, Y. Park, and S.H. Lee, "Synthesis of Cell-Laden Alginate Hollow Fibers Using Microfluidic Chips and Microvascularized Tissue-Engineering Applications", Small, vol. 5, pp. 1264-1268, Jun 5 2009. https://doi.org/10.1002/smll.200801667
  13. K.H. Lee, S.J. Shin, C.B. Kim, J.K. Kim, Y.W. Cho, B.G. Chung, et al., "Microfluidic synthesis of pure chitosan microfibers for bio-artificial liver chip", Lab on a Chip, vol. 10, pp. 1328-1334, 2010. https://doi.org/10.1039/b924987g
  14. J.M. Zhu and R.E. Marchant, "Design properties of hydrogel tissue-engineering scaffolds", Expert Review of Medical Devices, vol. 8, pp. 607-626, Sep 2011. https://doi.org/10.1586/erd.11.27
  15. K.A. Heintz, M.E. Bregenzer, J.L. Mantle, K.H. Lee, J.L. West, and J.H. Slater, "Fabrication of 3D Biomimetic Microfluidic Networks in Hydrogels", Adv Healthc Mater, May 30 2016.
  16. J.W. Nichol, S.T. Koshy, H. Bae, C.M. Hwang, S. Yamanlar, and A. Khademhosseini, "Cell-laden microengineered gelatin methacrylate hydrogels", Biomaterials, vol. 31, pp. 5536-5544, Jul 2010. https://doi.org/10.1016/j.biomaterials.2010.03.064
  17. B.G. Chung, K.H. Lee, A. Khademhosseini, and S.H. Lee, "Microfluidic fabrication of microengineered hydrogels and their application in tissue engineering", Lab on a Chip, vol. 12, pp. 45-59, 2012. https://doi.org/10.1039/C1LC20859D
  18. C.R. Wan, S. Chung, and R.D. Kamm, "Differentiation of Embryonic Stem Cells into Cardiomyocytes in a Compliant Microfluidic System", Annals of Biomedical Engineering, vol. 39, pp. 1840-1847, Jun 2011. https://doi.org/10.1007/s10439-011-0275-8
  19. P. Lee, R. Lin, J. Moon, and L.P. Lee, "Microfluidic alignment of collagen fibers for in vitro cell culture", Biomedical Microdevices, vol. 8, pp. 35-41, Mar 2006. https://doi.org/10.1007/s10544-006-6380-z
  20. H. Onoe, T. Okitsu, A. Itou, M. Kato-Negishi, R. Gojo, D. Kiriya, et al., "Metre-long cell-laden microfibres exhibit tissue morphologies and functions", Nature Materials, vol. 12, pp. 584-590, Jun 2013. https://doi.org/10.1038/nmat3606
  21. E. Hesse, T.E. Hefferan, J.E. Tarara, C. Haasper, R. Meller, C. Krettek, et al., "Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells", Journal of Biomedical Materials Research Part A, vol. 94A, pp. 442-449, Aug 2010.