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A study on the optimal variable transformation method to identify the correlation between ATP and APC

ATP와 APC 간의 관련성 규명을 위한 최적의 변수변환법에 관한 연구

  • 문혜경 (창원대학교 자연과학대학 식품영양학과) ;
  • 신재경 (창원대학교 자연과학대학 통계학과) ;
  • 김양숙 (창원 반동초등학교)
  • Received : 2016.08.24
  • Accepted : 2016.10.10
  • Published : 2016.11.30

Abstract

In order to secure safe meals, the hazards of microorganisms associated with food poisoning accident should be monitored and controlled in real situations. It is necessary to determined the correlation between existing common bacteria number (aerobic plate count; APC) and RLU (relative light unit) in cookware. In this paper, we investigate the correlation between ATP (RUL) and APC (CFU) by using three types of transform (inverse, square root, log transforms) of raw data in two steps. Among these transforms, the log transform at the first step has been found to be optimal for the data of cutting board, knife, soup bowl (stainless), and tray (carbon). The square root-inverse and the square root-square root transform at the second step have been shown to be optimal respectively for the cup and for the soup bowl (carbon) data.

안전한 급식을 위해서는 식중독사고와 연관성이 있는 미생물의 위해요소들이 실시간적으로 모니터링 되고 통제되어야 한다. 선진국에서는 실시간 위생 모니터링 도구로 ATP (adenosine triphosphate) Luminometer를 활용한 사례가 여러 건 보고되었다. ATP analyser는 ATP bioluminescence (생물발광성)의 원리를 이용하여 RLU (relatively light unit) 값으로 위생수준을 간접적으로 측정할 수 있게 해준다고 알려져 있다. 이에 국내 급식산업에서도 이를 활용할 수 있도록 조리도구들을 대상으로 일반세균수 (aerobic plate count; APC)와 RLU 간에 상관성이 존재하는지를 확인 할 필요성이 제기 되었다. 본 연구는 급식소의 조리도구 표면에 사전 처리없이 ATP (RLU)와 APC (CFU)를 측정하여 상관관계 존재 여부 파악 및 이들 관계의 최적인 모델을 찾아보고자 하였다. 이들에 대한 분석 결과를 조리도구별로 요약하면 다음과 같다. 도마, 칼, 국그릇 (스텐), 식판 (카보) 자료는 1차 변환의 로그변환이, 컵 자료는 2차 변환의 제곱근-역변환 (혹은 역-제곱근변환)이, 국그릇 (카보) 자료는 2차 변환의 제곱근-제곱근변환이 표준화 회귀계수 및 결정계수 $R^2$가 가장 좋게 나타났으나 식판 (스텐) 자료는 원자료, 1차 변환 및 2차 변환 모든 경우에서 정규성을 만족하지 못하여 이번 자료에서는 최적인 경우를 찾을 수가 없었다.

Keywords

References

  1. Bautista, D. A., Vaillancourt, J. P., Clarke, R. A., Renwick, S. and Griffiths, M. W. (1995). Rapid assessment of the microbiological quality of poultry carcasses using ATP bioluminescence. Journal of Food Protection, 58, 551-554. https://doi.org/10.4315/0362-028X-58.5.551
  2. Bell, C., Bowles, C. D., Toszeghy, M. J. K. and Neaves, P. (1996). Development of a hygiene standard for raw milk based on the lumac ATP bioluminescence method. International Dairy Journal, 6, 709-713. https://doi.org/10.1016/0958-6946(96)00007-6
  3. Hwang, E. G., Kim, S. J., Kim, B. K. and Lee, J. Y. (2015). Awareness analysis for popularization of temple food in monks. Journal of the Korean Data & Information Science Society, 26, 1217-1224. https://doi.org/10.7465/jkdi.2015.26.6.1217
  4. Jo, J. N. and Chang, U. J. (2013). A statistical analysis of the fat mass repeated measures data using mixed model. Journal of the Korean Data & Information Science Society, 24, 303-310. https://doi.org/10.7465/jkdi.2013.24.2.303
  5. Kim, B. Y., Song, H. Y., Park, I. S., Kim, Y. S., Lee, Y. S. and Ha, S. D. (2009). A correlation study of surveillance data and ATP bioluminescence assay for verification of hygienic status in major hotel in Seoul. Journal of Food Hygiene and Safety, 24, 277-284.
  6. Kim, Y. S., Moon, H. K., Kang, S. I. and Nam, E. J. (2010). Verification of suitability of ATP luminometer as the monitoring tool for surface hygiene in foodservices. Journal of the Korean Society of Food Science and Nutrition, 39, 1719-1723. https://doi.org/10.3746/jkfn.2010.39.11.1719
  7. Lee, M. K. (2015). A study on differences by gender in influence of recognition, usage, convenience and preference on purchase intention for traditional Kotean rice cake. Journal of the Korean Data & Information Science Society, 26, 875-883. https://doi.org/10.7465/jkdi.2015.26.4.875
  8. Marena, C., Lodola, L., Zecca, M., Bulgheroni, A., Carretto, E., Maserati, R. and Zambianchi, L. (2002). Assessment of handwashing practices with chemical and microbiologic methods : Preliminary results from a prospective crossover study. American Journal Infect Control, 30, 334-340. https://doi.org/10.1067/mic.2002.125809
  9. Ryan, R. A. (1996). Bioluminescence testing of aseptically packaged friut juice. Milk Industry International, 98, 13-15.
  10. Sea, S. H., Moon, S. J. and Choi, J. H. (2011). Evaluation of hygienic status using ATP bioluminescence assay and food service workers' sanitation performance in elderly welfare facilities. Journal of Korean Dietetic Association, 17, 277-284.
  11. Sea, Y. J. and Jeon, M. S. (2015). Effects of an education program on sanitation status at centers for children's food service management - Focusing on Jung-gu and Dong-gu regions of Daejeon Metropolitan City. Korean Journal of Community Nutrition, 20, 447-459. https://doi.org/10.5720/kjcn.2015.20.6.447
  12. Turantas, F. (1996). ATP bioluminescence method and applications in food microbiology. Gida, 21, 331-335.
  13. Werlein, H. D. and Fricke, R. (1997). Applicability of the swab sampling technique in order to determine the microbial quality of poultry by means of ATP bioluminescence. Archiv fuer Lebensmittelhygiene, 48, 14-16.