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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Misexpression of AtTX12 encoding a Toll/interleukin-1 receptor domain induces growth defects and expression of defense-related genes partially independently of EDS1 in Arabidopsis

  • Received : 2016.10.24
  • Accepted : 2016.11.02
  • Published : 2016.12.31
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Abstract

In this study, a tissue-specific GAL4/UAS activation tagging system was used for the characterization of genes which could induce lethality when ubiquitously expressed. A dominant mutant exhibiting stunted growth was isolated and named defective root development 1-D (drd1-D). The T-DNA tag was located within the promoter region of AtTX12, which is predicted to encode a truncated nucleotide-binding leucine-rich repeat (NLR) protein, containing a Toll/interleukin-1 receptor (TIR) domain. The transcript levels of AtTX12 and defense-related genes were elevated in drd1-D, and the misexpression of AtTX12 recapitulated the drd1-D phenotypes. In the presence of ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), a key transducer of signals triggered by TIR-type NLRs, a low-level of AtTX12 misexpression induced strong defective phenotypes including seedling lethality whereas, in the absence of EDS1, a high-level of AtTX12 misexpression induced weak growth defects like dwarfism, suggesting that AtTX12 might function mainly in an EDS1-dependent and partially in an EDS1-independent manner.

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References

  1. DeYoung BJ and Innes RW (2006) Plant NBS-LRR proteins in pathogen sensing and host defense. Nat Immunol 7, 1243-1249 https://doi.org/10.1038/ni1410
  2. Jones JD and Dangl JL (2006) The plant immune system. Nature 444, 323-329 https://doi.org/10.1038/nature05286
  3. Meyers BC, Dickerman AW, Michelmore RW, Sivaramak-rishnan S, Sobral BW and Young ND (1999) Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily. Plant J 20, 317-332 https://doi.org/10.1046/j.1365-313X.1999.t01-1-00606.x
  4. Meyers BC (2003) Genome-Wide Analysis of NBS-LRR-Encoding Genes in Arabidopsis. Plant Cell 15, 809-834 https://doi.org/10.1105/tpc.009308
  5. Falk A, Feys BJ, Frost LN, Jones JD, Daniels MJ and Parker JE (1999) EDS1, an essential component of R gene-mediated disease resistance in Arabidopsis has homology to eukaryotic lipases. Proc Natl Acad Sci U S A 96, 3292-3297 https://doi.org/10.1073/pnas.96.6.3292
  6. Jirage D, Tootle TL, Reuber TL et al (1999) Arabidopsis thaliana PAD4 encodes a lipase-like gene that is important for salicylic acid signaling. Proc Natl Acad Sci U S A 96, 13583-13588 https://doi.org/10.1073/pnas.96.23.13583
  7. Aarts N, Metz M, Holub E, Staskawicz BJ, Daniels MJ and Parker JE (1998) Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis. Proc Natl Acad Sci U S A 95, 10306-10311 https://doi.org/10.1073/pnas.95.17.10306
  8. Wiermer M, Feys BJ and Parker JE (2005) Plant immunity: the EDS1 regulatory node. Curr Opin Plant Biol 8, 383-389 https://doi.org/10.1016/j.pbi.2005.05.010
  9. Bernoux M, Ve T, Williams S et al (2011) Structural and functional analysis of a plant resistance protein TIR domain reveals interfaces for self-association, signaling, and autoregulation. Cell Host Microbe 9, 200-211 https://doi.org/10.1016/j.chom.2011.02.009
  10. Williams SJ, Sohn KH, Wan L et al (2014) Structural basis for assembly and function of a heterodimeric plant immune receptor. Science 344, 299-303 https://doi.org/10.1126/science.1247357
  11. Sukarta OC, Slootweg EJ and Goverse A (2016) Structure-informed insights for NLR functioning in plant immunity. Semin Cell Dev Biol 56, 134-149 https://doi.org/10.1016/j.semcdb.2016.05.012
  12. Frost D, Way H, Howles P et al (2004) Tobacco transgenic for the flax rust resistance gene L expresses allele-specific activation of defense responses. Mol Plant Microbe Interact 17, 224-232 https://doi.org/10.1094/MPMI.2004.17.2.224
  13. Swiderski MR, Birker D and Jones JD (2009) The TIR domain of TIR-NB-LRR resistance proteins is a signaling domain involved in cell death induction. Mol Plant Microbe Interact 22, 157-165 https://doi.org/10.1094/MPMI-22-2-0157
  14. Michael Weaver L, Swiderski MR, Li Y and Jones JD (2006) The Arabidopsis thaliana TIR-NB-LRR R-protein, RPP1A; protein localization and constitutive activation of defence by truncated alleles in tobacco and Arabidopsis. Plant J 47, 829-840 https://doi.org/10.1111/j.1365-313X.2006.02834.x
  15. Meyers BC, Morgante M and Michelmore RW (2002) TIR-X and TIR-NBS proteins: two new families related to disease resistance TIR-NBS-LRR proteins encoded in Arabidopsis and other plant genomes. Plant J 32, 77-92 https://doi.org/10.1046/j.1365-313X.2002.01404.x
  16. Kato H, Saito T, Ito H, Komeda Y and Kato A (2014) Overexpression of the TIR-X gene results in a dwarf phenotype and activation of defense-related gene expression in Arabidopsis thaliana. J Plant Physiol 171, 382-388 https://doi.org/10.1016/j.jplph.2013.12.002
  17. Nandety RS, Caplan JL, Cavanaugh K et al (2013) The role of TIR-NBS and TIR-X proteins in plant basal defense responses. Plant Physiol 162, 1459-1472 https://doi.org/10.1104/pp.113.219162
  18. Weigel D, Ahn JH, Blazquez MA et al (2000) Activation tagging in Arabidopsis. Plant Physiol 122, 1003-1013 https://doi.org/10.1104/pp.122.4.1003
  19. Waki T, Miyashima S, Nakanishi M, Ikeda Y, Hashimoto T and Nakajima K (2013) A GAL4-based targeted activation tagging system in Arabidopsis thaliana. Plant J 73, 357-367 https://doi.org/10.1111/tpj.12049
  20. Uknes S, Mauch-Mani B, Moyer M et al (1992) Acquired resistance in Arabidopsis. Plant Cell 4, 645-656 https://doi.org/10.1105/tpc.4.6.645
  21. Alcazar R and Parker JE (2011) The impact of temperature on balancing immune responsiveness and growth in Arabidopsis. Trends Plant Sci 16, 666-675 https://doi.org/10.1016/j.tplants.2011.09.001
  22. Burgos-Rivera B and Dawe RK (2012) An Arabidopsis tissue-specific RNAi method for studying genes essential to mitosis. PLoS One 7, e51388 https://doi.org/10.1371/journal.pone.0051388
  23. Bartsch M, Gobbato E, Bednarek P et al (2006) Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the Nudix hydrolase NUDT7. Plant Cell 18, 1038-1051 https://doi.org/10.1105/tpc.105.039982
  24. Zhao T, Rui L, Li J et al (2015) A truncated NLR protein, TIR-NBS2, is required for activated defense responses in the exo70B1 mutant. PLoS Genet 11, e1004945 https://doi.org/10.1371/journal.pgen.1004945
  25. Nguyen Ba AN, Pogoutse A, Provart N and Moses AM (2009) NLStradamus: a simple Hidden Markov Model for nuclear localization signal prediction. BMC Bioinformatics 10, 202 https://doi.org/10.1186/1471-2105-10-202
  26. Wirthmueller L, Zhang Y, Jones JD and Parker JE (2007) Nuclear accumulation of the Arabidopsis immune receptor RPS4 is necessary for triggering EDS1-dependent defense. Curr Biol 17, 2023-2029 https://doi.org/10.1016/j.cub.2007.10.042
  27. Feys BJ, Wiermer M, Bhat RA et al (2005) Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate immunity. Plant Cell 17, 2601-2613 https://doi.org/10.1105/tpc.105.033910
  28. Rietz S, Stamm A, Malonek S et al (2011) Different roles of Enhanced Disease Susceptibility1 (EDS1) bound to and dissociated from Phytoalexin Deficient4 (PAD4) in Arabidopsis immunity. New Phytol 191, 107-119 https://doi.org/10.1111/j.1469-8137.2011.03675.x
  29. Kang YH, Kirik V, Hulskamp M et al (2009) The MYB23 gene provides a positive feedback loop for cell fate specification in the Arabidopsis root epidermis. Plant Cell 21, 1080-1094 https://doi.org/10.1105/tpc.108.063180