Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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An Annealing Control Primer (ACP) System Used for the Isolation and Identification of Copper-Induced Genes in Alfalfa Leaves

  • Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Ki-Yong (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ji, Hee Chung (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Hyung Soo (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hwang, Tae Young (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Gi Jun (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Rahman, Md. Atikur (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2016.08.15
  • Accepted : 2016.09.05
  • Published : 2016.09.30
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Abstract

Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

Keywords

References

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