Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Elemental analysis of caries-affected root dentin and artificially demineralized dentin

  • Sung, Young-Hye (Department of Dental Science, Graduate School, Seoul National University) ;
  • Son, Ho-Hyun (Department of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Yi, Keewook (Korea Geochronology Team, Korea Basic Science Institute) ;
  • Chang, Juhea (Special Care Clinic, Seoul National University Dental Hospital, Dental Research Institute, Seoul National University)
  • Received : 2015.11.23
  • Accepted : 2016.06.22
  • Published : 2016.11.30
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Abstract

Objectives: This study aimed to analyze the mineral composition of naturally- and artificially-produced caries-affected root dentin and to determine the elemental incorporation of resin-modified glass ionomer (RMGI) into the demineralized dentin. Materials and Methods: Box-formed cavities were prepared on buccal and lingual root surfaces of sound human premolars (n = 15). One cavity was exposed to a microbial caries model using a strain of Streptococcus mutans. The other cavity was subjected to a chemical model under pH cycling. Premolars and molars with root surface caries were used as a natural caries model (n = 15). Outer caries lesion was removed using a carbide bur and a hand excavator under a dyeing technique and restored with RMGI (FujiII LC, GC Corp.). The weight percentages of calcium (Ca), phosphate (P), and strontium (Sr) and the widths of demineralized dentin were determined by electron probe microanalysis and compared among the groups using ANOVA and Tukey test (p < 0.05). Results: There was a pattern of demineralization in all models, as visualized with scanning electron microscopy. Artificial models induced greater losses of Ca and P and larger widths of demineralized dentin than did a natural caries model (p < 0.05). Sr was diffused into the demineralized dentin layer from RMGI. Conclusions: Both microbial and chemical caries models produced similar patterns of mineral composition on the caries-affected dentin. However, the artificial lesions had a relatively larger extent of demineralization than did the natural lesions. RMGI was incorporated into the superficial layer of the caries-affected dentin.

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References

  1. Heijnsbroek M, Paraskevas S, Van der Weijden GA. Fluoride interventions for root caries: a review. Oral Health Prev Dent 2007;5:145-152.
  2. Amer RS, Kolker JL. Restoration of root surface caries in vulnerable elderly patients: a review of the literature. Spec Care Dent 2013;33:141-149. https://doi.org/10.1111/j.1754-4505.2012.00302.x
  3. Perdigㅁo J. Dentin bonding-variables related to the clinical situation and the substrate treatment. Dent Mater 2010;26:e24-e37.
  4. Ngo H, Ruben J, Arends J, White D, Mount GJ, Peters MC, Faller RV, Pfarrer A. Electron probe microanalysis and transverse microradiography studies of artificial lesions in enamel and dentin: a comparative study. Adv Dent Res 1997;11:426-432. https://doi.org/10.1177/08959374970110040801
  5. Ngo HC, Mount G, McIntyre J, Do L. An in vitro model for the study of chemical exchange between glass ionomer restorations and partially demineralized dentin using a minimally invasive restorative technique. J Dent 2011;39(Supplement 2):S20-S26. https://doi.org/10.1016/j.jdent.2011.10.016
  6. Hevinga MA, Opdam NJ, Frencken JE, Truin GJ, Huysmans MC. Does incomplete caries removal reduce strength of restored teeth? J Dent Res 2010;89:1270-1275. https://doi.org/10.1177/0022034510377790
  7. ten Cate JM, Buijs MJ, Miller CC, Exterkate RA. Elevated fluoride products enhance remineralization of advanced enamel lesions. J Dent Res 2008;87:943-947. https://doi.org/10.1177/154405910808701019
  8. Kidd EA, Fejerskov O. What constitutes dental caries? Histopathology of carious enamel and dentin related to the action of cariogenic biofilms. J Dent Res 2004;83(Supplement 1):C35-C38.
  9. Schwendicke F, Meyer-Lueckel H, Schulz M, Dorfer CE, Paris S. Radiopaque tagging masks caries lesions following incomplete excavation in vitro. J Dent Res 2014;93:565-570. https://doi.org/10.1177/0022034514531291
  10. Pugach MK, Strother J, Darling CL, Fried D, Gansky SA, Marshall SJ, Marshall GW. Dentin caries zones: mineral, structure, and properties. J Dent Res 2009;88:71-76. https://doi.org/10.1177/0022034508327552
  11. Joves GJ, Inoue G, Nakashima S, Sadr A, Nikaido T, Tagami J. Mineral density, morphology and bond strength of natural versus artificial caries-affected dentin. Dent Mater J 2013;32:138-143. https://doi.org/10.4012/dmj.2012-243
  12. Shen S, Samaranayake LP, Yip HK. In vitro growth, acidogenicity and cariogenicity of predominant human root caries flora. J Dent 2004;32:667-678. https://doi.org/10.1016/j.jdent.2004.07.002
  13. McComb D, Erickson RL, Maxymiw WG, Wood RE. A clinical comparison of glass ionomer, resin-modified glass ionomer and resin composite restorations in the treatment of cervical caries in xerostomic head and neck radiation patients. Oper Dent 2002;27:430-437.
  14. De Moor RJ, Stassen IG, van't Veldt Y, Torbeyns D, Hommez GM. Two-year clinical performance of glass ionomer and resin composite restorations in xerostomic head- and neck-irradiated cancer patients. Clin Oral Investig 2011;15:31-38. https://doi.org/10.1007/s00784-009-0355-4
  15. Coutinho E, Yoshida Y, Inoue S, Fukuda R, Snauwaert J, Nakayama Y, De Munck J, Lambrechts P, Suzuki K, Van Meerbeek B. Gel phase formation at resin-modified glassionomer/tooth interfaces. J Dent Res 2007;86:656-661. https://doi.org/10.1177/154405910708600714
  16. AL-Helal AS, Armstrong SR, Xie XJ, Wefel JS. Effect of smear layer on root demineralization adjacent to resinmodified glass ionomer. J Dent Res 2003;82:146-150. https://doi.org/10.1177/154405910308200214
  17. Ab-Ghani Z, Ngo H, McIntyre J. Effect of remineralization/demineralization cycles on mineral profiles of Fuji IX Fast in vitro using electron probe microanalysis. Aust Dent J 2007;52:276-281. https://doi.org/10.1111/j.1834-7819.2007.tb00502.x
  18. Borczyk D. Piatowska D, Krzeminski Z. An in vitro study of affected dentin as a risk factor for the development of secondary caries. Caries Res 2006;40:47-51. https://doi.org/10.1159/000088906
  19. Boston DW, Liao J. Staining of non-carious human coronal dentin by caries dyes. Oper Dent 2004;29:280-286.

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