Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Determination of best enrichment media for growth of Salmonella injured from cold temperature during process and storage

저온저장으로 인해 손상된 살모넬라를 배양하기 위한 최적의 배지 선정에 관한 연구

  • Park, Mi-Kyung (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2016.08.24
  • Accepted : 2016.10.28
  • Published : 2016.11.30
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Abstract

This purpose of this study was to determine the best enrichment medium for rejuvenating and recovering Salmonella placed in cold temperature prior to the employment of the gold biosensor combined with a light microscopic imaging system. A mixture of nalidixic-resistant Salmonella Typhimurium and Enteritidis were inoculated onto chicken (1,000 CFU/chicken). After cold injury at $4^{\circ}C$ for 24 hr, Salmonella on chicken was enriched for 6 hr with six non-selective media including buffered peptone water broth, lactose broth, brain heart infusion broth (BHI), universal pre-enrichment broth, nutrient broth, and tryptic soy broth, and five selective media including brilliant green broth (BG), rappaport-vassiliadis R10 broth, selenite cystine broth, selenite broth, and tetrathionate brilliant green broth (TBG) for the comparison of Salmonella growth. Various concentrations of Salmonella (10, 50, 100, 500, and 1,000 CFU/chicken) were then enriched for 6 hr in both BHI and BG media to select the best media. BHI was selected as the most effective non-selective enrichment medium, while BG was selected as the most effective selective enrichment medium. Finally, BHI medium was selected as the most efficient enrichment medium for Salmonella growth injured from cold temperature during processing or storage.

본 연구의 목적은 가금류에서 문제가 되고 있는 살모넬라 신속검출을 위해 광학현미경 기반 이미징 시스템 적용에 앞서서 냉장온도로부터 손상된 살모넬라를 증균시키기 위한 최적의 배지를 선정하는 것이다. 대표적인 식중독 유발균주인 S. Typhimurium과 S. Enteritidis를 닭에 도말 하였고 $4^{\circ}C$에서 24시간 동안 저장한 후 BPW, LB, BHI, UPB, NB, 그리고 TSB 배지와 BG, RV, SC, SB 그리고 TBS 선택배지에 각각 6시간 동안 배양하였다. 배양 후, 2시간마다 살모넬라 균수를 측정하였고 그 결과 BHI와 BG를 선택되었다. 최종적으로 최적의 배지 선택을 위해 다양한 농도의 살모넬라를 닭에 각각 도말 후 6시간 동안 배양하면서 균수를 변화를 측정하였다. 그 결과 BHI가 냉해로부터 손상된 살모넬라를 증균시키기 위한 가장 최적의 배지로 선정되었으며 본 연구의 결과는 광학현미경 기반 이미징 시스템을 활용한 신속검출법 적용을 위한 증균배지로 이용될 것이다.

Keywords

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