Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant capacity and Raw 264.7 macrophage anti-inflammatory effect of the Tenebrio Molitor

갈색거저리(Tenebrio Molitor)의 항산화능과 Raw 264.7 대식세포의 항염증 효과

  • Yu, Jae-Myo (Department of Cosmeceutical Science, DaeguHanny University) ;
  • Jang, Jae-Yoon (Department of Cosmeceutical Science, DaeguHanny University) ;
  • Kim, Hyeon-Jeong (Department of Cosmeceutical Science, DaeguHanny University) ;
  • Cho, Yong-Hun (Department of Cosmeceutical Science, DaeguHanny University) ;
  • Kim, Dong-in (Department of Cosmeceutical Science, DaeguHanny University) ;
  • Kwon, O-jun (Regional industry Evaluation Agency for Gyeongbuk) ;
  • Cho, Yeong-Je (School of Food Science and Biotechnology / Food and Bio-Industry Research Institute, Kyungpook National University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, DaeguHanny University)
  • 유재묘 (대구한의대학교 화장품약리학과) ;
  • 장재윤 (대구한의대학교 화장품약리학과) ;
  • 김현정 (대구한의대학교 화장품약리학과) ;
  • 조용훈 (대구한의대학교 화장품약리학과) ;
  • 김동인 (대구한의대학교 화장품약리학과) ;
  • 권오준 (경북지역산업평가단) ;
  • 조영제 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2016.04.21
  • Accepted : 2016.08.17
  • Published : 2016.11.30
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Abstract

The purpose of this paper is to investigate potential anti-inflammatory and anti-oxidant effects of Tenebrio molitor. Macrophage cell response by outside stimulation leads expression of pro-inflammatory cytokines, such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), interleukin-6 (IL-6), $interleukin-1{\beta}$ ($IL-1{\beta}$), and trigger expression of genes which are affected by inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), resulting in formation of inflammatory factors like nitric oxide (NO) and Prostaglandin $E_2$ (PGE2). Cell viability was determined by MTT assay. In order to investigate anti-inflammatory agents, the inhibitory effects on the production of lipopolysaccharide (LPS)-induced NO in RAW 264.7 cells were examined. T. Molitor significantly decreased the production of NO in a dose-dependent manner, and also reduced the expression of iNOS, a COX-2 protein. As a result, the levels of protein such as $PGE_2$, iNOS, COX-2 and MARKs were significantly reduced compared to non-treated group in T. Molitor water extract (TDW) treated group. Also, antioxidant effect of T. Molitor were investigated using DPPH, ABTS+ and superoxide anion radical scavenging activity tests in cell-free system. Antioxidant activity of T. molitor was found low in the DPPH radical scavenging test while high in the ABTS+ and superoxide anion radical scavenging activity tests. These results show that TDW could be an effective anti-pro-inflammatory and anti-oxidant agent.

본 연구의 목적은 갈색거저리의 추출물에 따른 약리활성에 대한 검증 및 효능 평가이다. 갈색거저리의 항산화, 항염증에 대하여 효과를 확인 하였다. 염증 반응은 자극이 가해지면 histamin, serotonin, prostaglandin과 같은 혈관 활성물질에 의해 혈관 투과성이 증대되어 염증을 유발하고 cytokine, free radical, lysosomal enzyme 등 다양한 매개 인자가 관여한다. 자극에 의한 macrophage cell의 염증반응은 tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$), interleukin-6(IL-6), $interleukin-1{\beta}$($IL-1{\beta}$)와 같은 pro-inflammatory cytokine의 발현이 유도되고, inducible nitric oxide synthase(iNOS)와 cyclooxygenase-2(COX-2)에 영향을 받는 유전자의 발현을 자극하게 되어 nitric oxide(NO) 및 $PGE_2$등의 염증 인자가 생성된다. 이에 따라 갈색거지리 추출물의 항염증에 대한 연구를 위해 이에 영향을 주는 인자인 iNOS, COX-2, $PGE_2$, MAPKs의 단백질 발현억제 작용을 확인 하였다. 그 결과 TDW 처리군에서 iNOS 발현율이 19.7%, COX-2의 발현율은 23.2%의 값을 나타내었고, $PGE_2$의 저해 경로를 보기 위해 COX-2의 발현을 mRNA 수준에서 측정한 결과 최고 농도인 $100{\mu}g/mL$에서는 약 60%의 억제효과를 확인할 수 있었다. 결론적으로 TDW는 염증 생성 기전에 작용하여 이 활성을 억제하는데 있어서 효과를 줄 수 있으며 지속적으로 연구해볼 가치가 있다고 사료된다.

Keywords

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