한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제45권12호
  • /
  • Pages.1701-1707
  • /
  • 2016
  • /
  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

고지방식이로 유도한 비만 Mice에서 잣송이 초임계 추출물의 항비만 효과

Anti-Obesity Effect of Pine Cone (Pinus koraiensis) Supercritical Extract in High-Fat Diet-Induced Obese Mice

  • 이다솜 (경희대학교 동서의학대학원 의학영양학과) ;
  • 이민희 (경희대학교 동서의학대학원 의학영양학과) ;
  • 김혜숙 (경희대학교 동서의학대학원 동서의학과) ;
  • 정특래 ((주)키토라이프) ;
  • 양현필 ((주)키토라이프) ;
  • 허석현 (한국건강기능식품협회) ;
  • 이정민 (경희대학교 동서의학대학원 의학영양학과)
  • 투고 : 2016.08.19
  • 심사 : 2016.09.20
  • 발행 : 2016.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 C57BL/6J 마우스에 고지방 식이를 통한 비만을 유도시키며 동시에 잣송이 초임계 추출물을 식이에 첨가하여 8주간 식이 투여하여 항비만 식품 소재로서의 기능성을 알아보고자 하였다. 잣송이 초임계 추출물 20 mg/kg b.w.(HFD+PC low), 잣송이 초임계 추출물 100 mg/kg b.w. (HFD+PC high) 섭취군의 체중증가량은 고지방식이 유도 비만 대조군(HFD)에 비해 각각 유의적으로 감소하였고, 간, 백색 지방, 총 지방 무게의 변화량 역시 잣송이 초임계 추출물 섭취군이 HFD군에 비해 유의적으로 감소하였다. 실험동물의 혈청 지질 함량은 총콜레스테롤, 중성지방, LDL/VLDL 콜레스테롤, HDL 콜레스테롤이 HFD군에 비해 잣송이 초임계 추출물 섭취군에서 유의적으로 감소하였으나, HDL/LDL 비율을 계산한 결과에서는 잣송이 초임계 추출물 섭취군이 HFD군에 비해 유의적으로 증가하였음을 확인하였다. 실험동물군의 지방 조직에서 adipogenic transcription factor ($PPAR-{\gamma}$, SREBP, C/EBP)의 발현을 측정한 결과, 잣송이 초임계 추출물 섭취군이 HFD군에 비해 유의적으로 감소하였다. 또한, adipogenic enzyme(FAS, LPL)의 발현을 측정한 결과, 잣송이 초임계 추출물 섭취군이 HFD군에 비해 유의적으로 감소하였다. 이러한 결과로부터 잣송이 초임계 추출물이 항비만 효과를 가진 천연 기능성 식품의 개발에 있어 기초 자료로 활용할 수 있을 것으로 기대할 수 있다.

The present study investigated the anti-obesity effect of pine cone (PC, Pinus koraiensis) supercritical extract in high-fat diet (HFD)-induced obese mice. Male C57BL/6J mice were treated with HFD, HFD+catechin, and HFD+PC [two different doses, 20 mg/kg body weight (b.w.) and 100 mg/kg b.w.] in each AIN93G supplement for 8 weeks. Treatment of HFD mice with both low and high doses of PC significantly reduced body weight gain compared to HFD mice. Liver weight of mice was reduced in both the low and high dose PC-supplemented groups (24.19% and 19.83%, respectively). Total adipose tissue weight of mice was reduced in both the low and high dose PC-supplemented groups (45.54% and 62.66%, respectively). Serum total cholesterol, triglyceride, LDL cholesterol, and HDL cholesterol were reduced in the low and high dose PC-supplemented groups, and ratios of HDL cholesterol to LDL cholesterol increased by 94.55% in the high dose PC-supplemented group. Serum leptin was significantly reduced in the low and high dose PC-supplemented groups (28.14% and 62.72%, respectively). These results were supported by genetic expression of protein and enzymes related to lipid metabolism assessed by real-time PCR. There was significant reduction of lipid regulatory transcription factors such as $PPAR-{\gamma}$, C/EBP, and SREBP and lipid enzymes such as fatty acid synthesis and lipoprotein lipase in the low and high dose PC-supplemented groups. However, there was no statistical difference between low and high dose PC treatments. These results suggest that pine cone supercritical extract supplementation is able to regulate serum lipid profiles by reducing total cholesterol, triglyceride, and LDL cholesterol levels, followed by regulation of expression of lipid metabolic factors, resulting in reduction of weight gain in HFD-induced obese mice.

키워드

참고문헌

  1. Korea Health Statistics. 2014. 2014: Korea National Health and Nutrition Examination Survey (KNHANES VI-2). Ministry of Health and Welfare, Seoul, Korea. p 50.
  2. Chua SC, Leilbel RL. 1996. Obesity genes: Molecular and metabolic mechanisms. Diabetes 5: 2-7.
  3. Berger J, Moller DE. 2002. The mechanisms of action of PPARs. Annu Rev Med 53: 409-435. https://doi.org/10.1146/annurev.med.53.082901.104018
  4. Horton JD, Goldstein JL, Brown MS. 2002. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest 109: 1125-1131. https://doi.org/10.1172/JCI0215593
  5. Ramji DP, Foka P. 2002. CCAAT/enhancer-binding proteins: structure, function and regulation. Biochem J 365: 561-575. https://doi.org/10.1042/bj20020508
  6. Spiegelman BM, Flier JS. 1996. Adipogenesis and obesity: rounding out the big picture. Cell 87: 377-389. https://doi.org/10.1016/S0092-8674(00)81359-8
  7. Kim DJ, Kwon CK, Choi DJ, Ka KH, Kim TM, Kim BT, Lee BK, Hwang JH, Ann ES, Kim DY. 2009. Effect of nutritional education and exercise intervention on improvement of diet intakes and metabolic risk factors in obese middle aged women. Korean J Exerc Nutr 13: 179-184.
  8. Ahn IS, Park KY, Do MS. 2007. Weight control mechanisms and antiobesity functional agents. J Korean Soc Food Sci Nutr 36: 503-513. https://doi.org/10.3746/jkfn.2007.36.4.503
  9. Mason EE. 1992. Methods for voluntary weight loss and control. Obes Surg 2: 275-276. https://doi.org/10.1381/096089292765560187
  10. Visscher TL, Seidell JC. 2001. The public health impact of obesity. Annu Rev Public Health 22: 355-375. https://doi.org/10.1146/annurev.publhealth.22.1.355
  11. Bray GA, Tartaglia LA. 2000. Medicinal strategies in the treatment of obesity. Nature 404: 672-677. https://doi.org/10.1038/35007544
  12. Apfelbaum M, Vague P, Ziegler O, Hanotin C, Thomas F, Leutenegger E. 1999. Long-term maintenance of weight loss after a very-low-calorie diet: a randomized blinded trial of the efficacy and tolerability of sibutramine. Am J Med 106: 179-184. https://doi.org/10.1016/S0002-9343(98)00411-2
  13. Yum KS. 2001. Orlistat (XenicalR). Korean J Obes 10: 25-36.
  14. Padwal RS, Majumdar SR. 2007. Drug treatments for obesity: orlistat, sibutramine, and rimonabant. Lancet 369: 71-77. https://doi.org/10.1016/S0140-6736(07)60033-6
  15. Zhi J, Moore R, Kanitra L, Mulligan TE. 2003. Effects of orlistat, a lipase inhibitor, on the pharmacokinetics of three highly lipophilic drugs (amiodarone, fluoxtine, and simvastatin) in healthy volunteers. J Clin Pharmacol 43: 428-435. https://doi.org/10.1177/0091270003252236
  16. Cercato C, Roizenblatt VA, Leanca CC, Segal A, Lopes Filho AP, Mancini MC, Halpern A. 2009. A randomized double-blind placebo-controlled study of the long-term efficacy and safety of diethylpropion in the treatment of obese subjects. Int J Obes 33: 857-865. https://doi.org/10.1038/ijo.2009.124
  17. Critchfield WB, Little EL Jr. 1996. Geographic distribution of the pines of the world. Forest Service, US Department of Agriculture, Washington, DC, USA. p 4.
  18. Lee SS, Choe YJ, Choe DH, Hong IP. 2003. Extractives of Pinus koraiensis wood. J Korean Wood Sci Technol 31: 48-49.
  19. Caldefie-Chezet F, Guerry M, Chalchat JC, Fusillier C, Vasson MP, Guillot J. 2004. Anti-inflammatory effects of Melaleuca alternifolia essential oil on human polymorphonuclear neutrophils and monocytes. Free Radic Res 38: 805-811. https://doi.org/10.1080/1071576042000220247
  20. Hwang HJ, Yu JS, Lee HY, Kwon DJ, Han W, Heo SI, Kim SY. 2014. Evaluations on deodorization effect and anti-oral microbial activity of essential oil from Pinus koraiensis. Korean J Plant Res 27: 1-10. https://doi.org/10.7732/kjpr.2014.27.1.001
  21. Ju YW, Lee MY, Woo MJ, Byun SY. 2005. The current status of supercritical fluid extraction technology and industrial application. Kor J Biotechnol Bioeng 20: 239-337.
  22. Ju YW, Kang MH. 2005. Production of functional sesame oil by supercritical fluid extraction. Food Industry and Nutrition 10(3): 22-29.
  23. Park PJ, Kim CW, Cho SY, Rha CS, Seo DB, Lee SJ. 2010. Aqueous spray-dried green tea extract regulates body weight and epididymal fat accumulation in mice. Korean J Food Sci Technol 42: 103-108.
  24. Green A, Rumberger JM, Stuart CA, Ruhoff MS. 2004. Stimulation of lipolysis by tumor necrosis factor-${\alpha}$ in 3T3-L1 adipocytes is glucose dependent: implications for long-term regulation of lipolysis. Diabetes 53: 74-81. https://doi.org/10.2337/diabetes.53.1.74
  25. Lee JS, Lee MK, Ha YT, Bok SH, Park HM, Jeong KS, Woo MN, Do GM, Yeo JY, Choi MS. 2006. Supplementation of whole persimmon leaf improves lipid profiles and suppressed body weight gain in rats fed high-fat diet. Food Chem Toxicol 44: 1875-1883. https://doi.org/10.1016/j.fct.2006.06.014
  26. Imano H, Noda H, Kitamura A, Sato S, Kiyama M, Sankai T, Ohira T, Nakamura M, Yamagishi K, Ikeda A, Shimamoto T, Iso H. 2011. Low-density lipoprotein cholesterol and risk of coronary heart disease among Japanese men and women: the Circulatory Risk in Communities Study (CIRCS). Prev Med 52: 381-386. https://doi.org/10.1016/j.ypmed.2011.02.019
  27. Gordon T, Castelli WP, Hjortland MC, Kannel WB, Dawber TR. 1977. High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. Am J Med 62: 707-716. https://doi.org/10.1016/0002-9343(77)90874-9
  28. Ikeda I, Imasato Y, Sasaki E, Nakayama M, Nagao H, Takeo T, Yayabe F, Sugano M. 1992. Tea catechins decrease micellar solubility and intestinal absorption of cholesterol in rats. Biochim Biophys Acta 1127: 141-146. https://doi.org/10.1016/0005-2760(92)90269-2
  29. Dallas C, Gerbi A, Tenca G, Juchaux F, Bernard FX. 2008. Lipolytic effect of a polyphenolic citrus dry extract of red orange, grapefruit, orange (SINETROL) in human body fat adipocytes. Mechanism of action by inhibition of cAMP-phosphodiesterase (PDE). Phytomedicine 15: 783-792. https://doi.org/10.1016/j.phymed.2008.05.006
  30. Tyagi S, Gupta P, Saini AS, Kaushal C, Sharma S. 2011. The peroxisome proliferator-activated receptor: A family of nuclear receptors role in various diseases. J Adv Pharm Technol Res 2: 236-240. https://doi.org/10.4103/2231-4040.90879
  31. Auwerx J, Leroy P, Schoonjans K. 1992. Lipoprotein lipase: recent contributions from molecular biology. Crit Rev Clin Lab Sci 29: 243-268. https://doi.org/10.3109/10408369209114602
  32. Ronnett GV, Kim EK, Landree LE, Tu Y. 2005. Fatty acid metabolism as a target for obesity treatment. Physiol Behav 85: 25-35. https://doi.org/10.1016/j.physbeh.2005.04.014

피인용 문헌

  1. 잣송이 추출물의 급여가 세미브로일러의 생산성, 소화기관 특성 및 장내 미생물에 미치는 영향 vol.46, pp.4, 2016, https://doi.org/10.5536/kjps.2019.46.4.305