Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Quality Characteristics and Antioxidant Activity of Espresso Coffee Prepared with Green Bean Fermented by Lactic Acid Bacteria

유산균 발효 생두를 이용한 에스프레소 커피의 품질 특성 및 항산화 활성

  • Kim, Dong-Ho (Division of Food and Animal Sciences, Chungbuk National University) ;
  • Yeon, Soo-Ji (Division of Food and Animal Sciences, Chungbuk National University) ;
  • Jang, Keum-Il (Division of Food and Animal Sciences, Chungbuk National University)
  • 김동호 (충북대학교 식품생명.축산과학부) ;
  • 연수지 (충북대학교 식품생명.축산과학부) ;
  • 장금일 (충북대학교 식품생명.축산과학부)
  • Received : 2016.08.12
  • Accepted : 2016.10.25
  • Published : 2016.12.31
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Abstract

This study investigated the quality characteristics and antioxidant activities of espresso coffee prepared with green bean fermented by lactic acid bacteria. First, 10, 20, and 30% (w/v) green beans were fermented by Lactobacillus acidophilus KCTC 3145 at $37^{\circ}C$ for 0, 12, and 24 h, respectively. Cells of L. acidophilus gradually increased with increasing green bean content and fermentation time. After drying fermented green beans, coffee powders were prepared by roasting (city level) and grinding (<75 mesh). Then, espresso coffee was extracted using coffee powder. The pH and chromaticity (L*, a*, and b* values) of espresso coffee decreased with fermentation time, whereas total acidity, total soluble solid contents, and brown color intensity increased. The pH level decreased with increasing contents of fermented green bean and total acidity increased. However, chromaticity, total soluble solid contents, and brown color intensity remained within a limited range. The antioxidant activities, including total polyphenol content, and DPPH and ABTS radical scavenging activities increased with increasing green bean content and fermentation time. Finally, sensory evaluation -for taste, color, flavor, and overall preference- revealed espresso coffee prepared with fermentation of 30% (w/v) green bean received the highest scores. Green bean fermented by lactic acid bacteria enhanced quality characteristics and antioxidant activities of espresso coffee, showing that lactic acid bacteria fermentation has potential use in the espresso coffee industry.

본 연구에서는 유산균으로 발효시킨 발효 생두를 이용하여 제조한 에스프레소 커피의 품질 및 항산화 효과를 분석하였다. 먼저 Lactobacillus acidophilus KCTC 3145를 이용하여 10, 20, 30%(w/v) 함량의 커피 생두를 $37^{\circ}C$에서 0, 12, 24시간 동안 발효시켰다. 그리고 발효된 커피 생두를 건조한 다음 로스팅 및 분쇄하여 얻은 커피 분말로 에스프레소 커피를 제조하여 pH, 산도, 가용성 고형분 함량, 갈색도, 색도, 항산화 활성 및 관능검사를 비교 분석하였다. 발효 중 유산균수의 변화는 발효를 위한 생두의 함량과 유산균 발효시간이 증가할수록 전체적으로 완만히 증가하는 경향을 나타내었다. 발효시간이 증가할수록 발효 생두를 이용한 에스프레소 커피의 pH 및 색도(L*, a*, b* 값)는 감소했지만, 총산도, 가용성 고형분 함량 및 갈색도는 증가하는 경향을 나타내었다. 발효 생두의 함량이 증가할수록 에스프레소 커피의 pH는 감소한 반면 총산도는 증가하였고, 색도, 가용성 고형분 함량 및 갈색도는 유사하게 나타났다. 그리고 항산화 효과에서는 발효시간 및 발효 생두의 함량이 증가할수록 총 폴리페놀 함량과 DPPH 및 ABTS 라디칼 소거능은 모두 증가하였으며, 관능특성으로 맛, 색, 향 및 전체적인 기호도에서 EFB30이 가장 높은 선호도를 나타내었다. 따라서 본 연구에서는 유산균 발효 생두를 이용하여 제조한 에스프레소 커피의 품질 및 항산화 특성을 상호 비교하고 관능적 특성을 확인함으로써 유산균 발효를 통한 에스프레소 커피의 품질 및 기능성 효과 향상 가능성에 대한 자료를 제시하였다고 생각된다.

Keywords

References

  1. Seo HS, Kang HJ, Jung EH, Hwang IK. 2006. Application of GC-SAW (surface acoustic wave) electronic nose classification of origins and blended commercial brands in roasted ground coffee beans. Korean J Food Cook Sci 22: 299-306.
  2. Park SS, Lee DH, Kim KI. 2012. A study on the quality of roast coffee bean according to methods of storage. Journal of the Korea Society for Coffee Industry 1: 31-36.
  3. Kim KH, Kim AH, Lee JK, Chun MS, Noh BS. 2014. Analysis of flavor pattern of various coffee beans using electronic nose. Korean J Food Sci Technol 46: 1-6. https://doi.org/10.9721/KJFST.2014.46.1.1
  4. Kim JY, Han YS. 2009. Influence of roasting time on antibacterial and antioxidative effects of coffee extract. Korean J Food Cook Sci 25: 496-505.
  5. Minamisawa M, Yoshida S, Takai N. 2004. Determination of biologically active substances in roasted coffees using a diode-array HPLC system. Anal Sci 20: 325-328. https://doi.org/10.2116/analsci.20.325
  6. Vignoli JA, Bassoli DG, Benassi MT. 2011. Antioxidant activity, polyphenols, caffeine and melanoidins in soluble coffee: The influence of processing conditions and raw material. Food Chem 124: 863-868. https://doi.org/10.1016/j.foodchem.2010.07.008
  7. Nkondjock A, Ghadirian P, Kotsopoulos J, Lubinski J, Lynch H, Kim-Sing C, Horsman D, Rosen B, Isaacs C, Weber B, Foulkes W, Ainsworth P, Tung N, Eisen A, Friedman E, Eng C, Sun P, Narod SA. 2006. Coffee consumption and breast cancer risk among BRCA1 and BRCA2 mutation carriers. Int J Cancer 118: 103-107. https://doi.org/10.1002/ijc.21296
  8. Hwang SH, Kim KS, Kang HJ, Kim MJ. 2013. Phenolic compound contents and antioxidative effects on dutch coffee by extraction time. Korean Public Health Research 39: 21-29.
  9. Park JY. 2013. Antioxidant activities and quality characteristics of pan bread with green coffee bean powder. MS Thesis. Sejong University, Seoul, Korea. p 26-42.
  10. Frisullo P, Laverse J, Barnaba M, Navarini L, Del Nobile MA. 2012. Coffee beans microstructural changes induced by cultivation processing: An X-ray microtomographic investigation. J Food Eng 109: 175-181. https://doi.org/10.1016/j.jfoodeng.2011.09.015
  11. Bhumiratana N, Adhikari K, Chambers E. 2011. Evolution of sensory aroma attributes from coffee beans to brewed coffee. LWT-Food Sci Technol 44: 2185-2192. https://doi.org/10.1016/j.lwt.2011.07.001
  12. Ann YG. 2011. [Lactic acid bacteria] Probiotic lactic acid bacteria. Korean J Food Nutr 24: 817-832. https://doi.org/10.9799/ksfan.2011.24.4.817
  13. Bang JH, Shin H, Choi HJ, Kim DW, Ahn CS, Jeong YK, Joo WH. 2012. Probiotic potential of Lactobacillus isolates. J Life Sci 22: 251-258. https://doi.org/10.5352/JLS.2012.22.2.251
  14. Kim JH, Lee WJ, Cho YW, Kim KY. 2009. Storage-life and palatability extension of Betula platyphylla sap using lactic acid bacteria fermentation. J Korean Soc Food Sci Nutr 38: 787-794. https://doi.org/10.3746/jkfn.2009.38.6.787
  15. Yeo MH, Kim DM, Kim YH, Kim JH, Baek H, Chung MJ. 2008. Antitumor activity of CBT-AK5 purified from Lactobacillus casei against Sarcoma-180 infected ICR mice. Korean J Dairy Sci Technol 26: 23-30.
  16. Kim SJ. 2005. Physicochemical characteristics of yogurt prepared with lactic acid bacteria isolated from Kimchi. Korean J Food Cult 20: 337-340.
  17. Lee Y, Chang HC. 2008. Isolation and characterization of kimchi lactic acid bacteria showing anti-Helicobacter pylori activity. Korean J Microbiol Biotechnol 36: 106-114.
  18. Kim JH, Oh MK, Rhee YH, Choi KC, Lee YK, Shin SY. 1999. Selection and physico-chemical characteristics of lactic acid bacteria which had cholesterol lowering activities. J Korean Soc Agric Chem Biotechnol 42: 83-90.
  19. Auh MS, Kim YS, Ahn SJ, Ahn JB, Kim KY. 2012. Comparison of property changes of black jujube and Zizyphus jujube extracts during lactic acid fermentation. J Korean Soc Food Sci Nutr 41: 1346-1355. https://doi.org/10.3746/jkfn.2012.41.10.1346
  20. Bong YJ, Jeong JK, Park KY. 2013. Fermentation properties and increased health functionality kimchi by kimchi lactic acid bacteria starters. J Korean Soc Food Sci Nutr 42: 1717-1726. https://doi.org/10.3746/jkfn.2013.42.11.1717
  21. Lee TJ, Hwang DY, Lee CY, Son HJ. 2009. Changes in yeast cell number, total acid and organic acid during production and distribution processes of Makgeolli, traditional alcohol of Korea. Korean J Microbiol 45: 391-396.
  22. Bravo J, Monente C, Juaniz I, De Pena MP, Cid C. 2013. Influence of extraction process on antioxidant capacity of spent coffee. Food Res Int 50: 610-616. https://doi.org/10.1016/j.foodres.2011.04.026
  23. Lim YT, Kim DH, Ahn JB, Choi SH, Han GP, Kim GH, Jang KI. 2012. Quality characteristics of madeleine with peach (Prunus persica L. Batsch) juice. Korean J Food Nutr 25: 664-670. https://doi.org/10.9799/ksfan.2012.25.3.664
  24. Dewanto V, Wu X, Liu RH. 2002. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50: 4959-4964. https://doi.org/10.1021/jf0255937
  25. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  26. Ahn JB, Park JA, Jo H, Woo I, Lee SH, Jang KI. 2012. Quality characteristics and antioxidant activity of commercial Doenjang and traditional Doenjang in Korea. Korean J Food Nutr 25: 142-148. https://doi.org/10.9799/ksfan.2012.25.1.142
  27. Chang CI, Lee JK, Ku KH, Kim WJ. 1990. Comparison of soybean varieties for yield, chemical and sensory properties of soybean curds. Korean J Food Sci Technol 22: 439-444.
  28. Hong HD, Sung SK, Rhee YK, Cho CW, Kim YC, Lee OH. 2013. Physicochemical properties and antioxidative activity of fermented Rhodiola sachalinensis and Korean red ginseng mixture by Lactobacillus acidophilus. Korean J Food Nutr 26: 358-365. https://doi.org/10.9799/ksfan.2013.26.3.358
  29. Han EH, Lee TS, Noh BS, Lee DS. 1997. Quality characteristics in mash of takju prepared by using different nuruk during fermentation. Korean J Food Sci Technol 29: 555-562.
  30. Kim GH, Bae EK. 1999. Lactic acid bacteria for the preservation of fruit and vegetable. Korean J Postharvest Sci Technol 6: 245-254.
  31. Park JY, Kim GJ, Kim KI. 2012. A study of chemical constituents between (Coffea aribica L.) defect beans and green beans. Journal of The Korea Society for Coffee Industry 1: 1-9.
  32. Yoon HH, Choi YM. 2009. Content of defective beans and cup quality in relation to the grade and processing methods of green coffee. Korean J Food Cook Sci 25: 703-711.
  33. Macrae R. 1985. Nitrogenous compounds. In Coffee Volume 1: Chemistry. Clarke RJ, Macrae R, eds. Elsevier Applied Science, Essex, UK. p 115-152.
  34. Wood LA, Shouse PJ. 1972. Standard reference materials: use of standard light-sensitive paper for calibrating carbon arcs used in testing textiles for colorfastness to light. National Bureau of Standards Special Publication 260-41, Washington, DC, USA. p 1-24.
  35. Mendes LC, Menezes HC, Aparecide M, Silva AP. 2001. Optimization of the roasting of robusta coffee (C. canephora conillon) using acceptability tests and RSM. Food Qual Prefer 12: 153-162. https://doi.org/10.1016/S0950-3293(00)00042-2
  36. Shin JY, Kim H, Kim DG, Baek GH, Jeong HS, Yu KW. 2013. Pharmacological activities of coffee roasted from fermented green coffee beans with fungal mycelia in solid-state culture. J Korean Soc Food Sci Nutr 42: 487-496. https://doi.org/10.3746/jkfn.2013.42.3.487
  37. Jeong HM, Kim YS, Ahn SJ, Auh MS, Ahn JB, Kim KY. 2011. Effects of Zizyphus jujuba var. boeunesis extracts on the growth of intestinal microflora and its antioxidant activities. J Korean Soc Food Sci Nutr 40: 500-508. https://doi.org/10.3746/jkfn.2011.40.4.500
  38. Choi MO, Kim BJ, Jo SK, Jung HK, Lee JT, Kim HY, Kweon DJ. 2013. Anti-allergic activities of Catanea crenata inner shell extracts fermented by Lactobacillus bifermentans. Korean J Food Preserv 20: 583-591. https://doi.org/10.11002/kjfp.2013.20.4.583
  39. Yang HS, Choi YJ, Oh HH, Moon JS, Jung HK, Ki KJ, Choi BS, Lee JW, Huh CK. 2014. Antioxidative activity of mushroom water extracts fermented by lactic acid bacteria. J Korean Soc Food Sci Nutr 43: 80-85. https://doi.org/10.3746/jkfn.2014.43.1.080
  40. Chae HJ, Park DI, Lee SC, Oh CH, Oh NS, Kim DC, Won SI, In MJ. 2011. Improvement of antioxidative activity by enzyme treatment and lactic acid bacteria cultivation in black garlic. J Korean Soc Food Sci Nutr 40: 660-664. https://doi.org/10.3746/jkfn.2011.40.5.660
  41. Choi YJ, Yang HS, Huh CK, Oh HH, Park TY, Kim MK, Jin SW, Seo KS, Jung HK. 2013. Quality characteristics and antioxidant activity of fermented milk containing mushroom extracts. Korean J Dairy Sci Technol 31: 187-194.
  42. Lindroth RL. 1988. Hydrolysis of phenolic glycosides by midgut ${\beta}$-glucosidases in Papilio glaucus subspecies. Insect Biochem 18: 789-792. https://doi.org/10.1016/0020-1790(88)90102-3
  43. Park JB, Sim SH, Ha SJ, Kim M. 2015. Enhancement of antioxidative activities of berry or vegetable juices through fermentation by lactic acid bacteria. Microbiol Biotechnol Lett 43: 291-295. https://doi.org/10.4014/mbl.1505.05007
  44. Lee YK, Kim M, Lee YC, Rho J, Ma JY, Cho CW. 2011. Characteristic changes of Galgeuntang fermented with lactic acid bacteria. Korean J Food Sci Technol 43: 655-658. https://doi.org/10.9721/KJFST.2011.43.5.655
  45. Yang MC, Jeong SW, Ma JY. 2011. Analysis of constituents in Sipjundaebo-tangs fermented by lactic acid bacteria. Korean J Microbiol Biotechnol 39: 350-356.

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