Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of High Purity β-1.3/1.6-Glucan on Macrophages, Natural Killer Cells, and T Cell-Mediated Factors

고순도 β-1.3/1.6-Glucan이 대식세포 및 자연살해세포와 T 세포면역계에 미치는 영향

  • Kwon, Hanol (Department of Medical Nutrition, Kyung Hee University) ;
  • Lee, Minhee (Department of Medical Nutrition, Kyung Hee University) ;
  • Park, Soo-Jeung (Department of Medical Nutrition, Kyung Hee University) ;
  • Lee, Dasom (Department of Medical Nutrition, Kyung Hee University) ;
  • Kim, Hyesook (Department of Medicine, Kyung Hee University) ;
  • Lee, Jeongmin (Department of Medical Nutrition, Kyung Hee University)
  • 권한올 (경희대학교 동서의학대학원 의학영양학과) ;
  • 이민희 (경희대학교 동서의학대학원 의학영양학과) ;
  • 박수정 (경희대학교 동서의학대학원 의학영양학과) ;
  • 이다솜 (경희대학교 동서의학대학원 의학영양학과) ;
  • 김혜숙 (경희대학교 동서의학대학원 의과학과) ;
  • 이정민 (경희대학교 동서의학대학원 의학영양학과)
  • Received : 2016.06.28
  • Accepted : 2016.08.16
  • Published : 2016.11.30
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Abstract

The present study investigated the immunomodulatory effects of high-purity ${\beta}$-1.3/1.6-glucan on macrophages, natural killer (NK) cells, and T cell-mediated factors. Effect of high-purity ${\beta}$-1.3/1.6-glucan on cytotoxicity in macrophages was investigated. Using macrophages, cytotoxicity of high-purity ${\beta}$-1.3/1.6-glucan was evaluated by MTT assay. We treated high-purity ${\beta}$-1.3/1.6-glucan at concentrations of 10, 50, 100, 150, 200, and $250{\mu}g/mL$ in macrophages. High-purity ${\beta}$-1.3/1.6-glucan did not affect macrophage viability. Phagocytic activity was assessed using zymosan. Activity of high-purity ${\beta}$-1.3/1.6-glucan on macrophages significantly increased as compared with zymosan. We treated high-purity ${\beta}$-1.3/1.6-glucan to murine NK cells co-incubated with YAC-1 cells. High-purity ${\beta}$-1.3/1.6-glucan resulted in significantly increased activity of NK cells as compared with the control. In addition, treatment of macrophages with high-purity ${\beta}$-1.3/1.6-glucan resulted in significantly increased activity of T cell-mediated cytokine (IL-2, IL-12, $IFN-{\gamma}$, and $TNF-{\alpha}$) levels and CD4+/CD8+ T cells as compared with the control. In conclusion, high-purity ${\beta}$-1.3/1.6-glucan could enhance the immune response through activation of macrophages, NK cells, and T cell-mediated factors.

본 연구에서는 고순도 ${\beta}$-1.3/1.6-glucan이 선천면역계에 중요한 역할을 하는 대식세포와 자연살해세포의 활성화와 적응면역계에서 중요한 역할을 하는 T 세포 면역계에 대한 면역조절 효과를 살펴보고자 대식세포의 활성능, 자연살해 세포의 활성능, 그리고 T 세포 면역계에 조절작용을 하는 사이토카인, CD4+/CD8+ T 세포에 미치는 영향을 관찰하였다. 마우스 복강에서 불리한 대식세포를 이용하여 세포독성을 확인한 결과 고순도 ${\beta}$-1.3/1.6-glucan $10{\sim}200{\mu}g/mL$의 농도에서 독성이 나타나지 않았다. 또한, 고순도 ${\beta}$-1.3/1.6-glucan은 대식세포의 활성능, 자연살해세포의 활성능에 도움을 주어 활성능을 증가시켜 외부로부터 침입한 미생물, 감염된 세포나 종양세포 등을 효과적으로 제거할 수 있을 것이라 예상할 수 있었다. 마지막으로 T 세포 면역계에 조절작용을 하는 사이토카인과 CD4+/CD8+를 확인한 결과 고순도 ${\beta}$-1.3/1.6-glucan이 사이토카인들의 분비량 및 CD4+/CD8+를 증가시켜 T 세포 면역계에 조절뿐아니라 B 세포 면역계의 조절에 도움을 줄 것이라 예상할 수 있었다. 결론적으로 고순도 ${\beta}$-1.3/1.6-glucan은 선천면역뿐 아니라 적응면역에서 영향을 미칠 것이라 생각하며 면역조절에 긍정적인 변화를 보였으므로 추후 면역 조절제로서 기능성 식품의 상업화에 기초 자료가 되어 국내 기능성 소재로서의 개발 가능성을 기대할 수 있다.

Keywords

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