Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Comparison of Antioxidant Activities of Rice Bran Extracts by Different Extraction Methods

추출방법에 따른 미강 추출물의 항산화 활성 비교

  • Ham, Hyeonmi (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Woo, Koan Sik (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Yu-Young (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Ji-Young (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Byongwon (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Yong-Hwan (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, In-Hwan (Department of Food and Nutrition, Korea University) ;
  • Lee, Junsoo (Department of Food and Animal Sciences, Chungbuk National University)
  • 함현미 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 우관식 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 이유영 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 박지영 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 이병원 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 최용환 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 김인환 (고려대학교 식품영양학과) ;
  • 이준수 (충북대학교 식품생명.축산과학부)
  • Received : 2016.07.19
  • Accepted : 2016.09.22
  • Published : 2016.11.30
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Abstract

The objective of this study was to determine the antioxidant activities of rice bran extracts by three different extraction methods. Rice bran was extracted by solvent extraction, saponification extraction, and supercritical fluid extraction. The antioxidant activities of the rice bran extracts were determined based on ABTS and DPPH radical scavenging activities, reducing power, and lipid peroxidation inhibitory activity. The unsaponifiable matter (USM) extracted by the saponification method showed higher ABTS (671.7 mg Trolox equivalent antioxidant capacity (TEAC)/g) and DPPH (330.7 mg TEAC/g) radical scavenging activities as well as reducing power ($A_{700}=1.14$) than those of the solvent extract (ME) and supercritical fluid extract (SFE). Inhibitory effect on lipid peroxidation was higher in USM (68.7%) and SFE (75.4%) compared to ME (47.8%). USM indicated relatively higher antioxidant activities compared with those of SFE and ME. These results show that the saponification method for extraction of USM from rice bran extracted was the most effective method for enhancement of antioxidant activity. In addition, these extracts from rice bran could be used as functional ingredients in the food industry.

본 연구에서는 미강의 산업적 이용 증대 및 고부가가치 소재개발을 목적으로 항산화 활성을 증가시킬 수 있는 추출 방법을 개발하기 위하여 용매 추출법, 검화 추출법 및 초임계 유체 추출법을 이용하여 제조한 미강 생리활성물질 농축물의 항산화 활성을 비교하였다. 항산화 활성은 ABTS 및 DPPH 라디칼 제거능, 환원력, 지질과산화 억제능을 이용하여 측정하였다. ABTS 및 DPPH 라디칼 제거능, 환원력 모두 unsaponifiable matter(USM)가 각각 671.7 mg TEAC/g, 330.7 mg TEAC/g 및 $A_{700}=1.14$로 가장 높은 활성을 나타내었다. 지질과산화 억제능은 USM 및 supercritical fluid extract(SFE)가 각각 68.7% 및 75.4%로 methanol extract(ME)의 47.8%보다 우수한 활성을 나타내었다. 따라서 검화 추출법으로 제조한 USM은 SFE 및 ME보다 상대적으로 우수한 항산화 활성을 가지며 미강을 기능성 소재로 개발하기 위한 추출 방법으로 활용 가능할 것으로 생각된다. 또한, 미강 생리활성물질 농축물은 기능성 증진을 위한 다양한 식품 소재로의 활용 가능성이 있을 것으로 생각된다.

Keywords

References

  1. Nagendra Prasad MN, Sanjay KR, Shravya Khatokar M, Vismaya MN, Nanjunda Swamy S. 2011. Health benefits of rice bran-A review. J Nutr Food Sci 1: 1-7.
  2. Hu W, Wells JH, Shin TS, Godber JS. 1996. Comparison of isopropanol and hexane for extraction of vitamin E and oryzanols from stabilized rice bran. J Am Oil Chem Soc 73: 1653-1656. https://doi.org/10.1007/BF02517967
  3. Oh SK, Kim DJ, Chun AR, Yoon MR, Kim KJ, Lee JS, Hong HC, Kim YK. 2010. Antioxidant compounds and antioxidant activities of ethanol extracts from milling by-products of rice cultivars. J Korean Soc Food Sci Nutr 39: 624-630. https://doi.org/10.3746/jkfn.2010.39.4.624
  4. Park JS, Kim MH. 2011. Anti-inflammatory effects of rice bran ethanol extract in murine macrophage RAW 264.7 cells. Yakhak Hoeji 55: 456-461.
  5. Nam SH, Choi SP, Kang MY, Kozukue N, Friedman M. 2005. Antioxidative, antimutagenic, and anticarcinogenic activities of rice bran extracts in chemical and cell assays. J Agric Food Chem 53: 816-822. https://doi.org/10.1021/jf0490293
  6. Revilla E, Maria CS, Miramontes E, Bautista J, Garcia-Martinez A, Cremades O, Cert R, Parrado J. 2009. Nutraceutical composition, antioxidant activity and hypocholesterolemic effect of a water-soluble enzymatic extract from rice bran. Food Res Int 42: 387-393. https://doi.org/10.1016/j.foodres.2009.01.010
  7. Hegsted M, Windhauser MM, Morris SK, Lester SB. 1993. Stabilized rice bran and oat bran lower cholesterol in humans. Nutr Res 13: 387-398. https://doi.org/10.1016/S0271-5317(05)80703-1
  8. Kim YH, Lee SS. 1995. The effect of diet containing different fiber sources on the serum lipid level and bowel function in rats. Korean J Nutr 28: 825-833.
  9. Kim JY, Shin M, Heo YR. 2014. Effects of stabilized rice bran on obesity and antioxidative enzyme activity in high fat diet-induced obese C57BL/6 mice. J Korean Soc Food Sci Nutr 43: 1148-1157. https://doi.org/10.3746/jkfn.2014.43.8.1148
  10. Choi HI, Ye EJ, Kim SJ, Bae MJ, Yee ST, Park EJ, Park EM. 2006. Anticancer (in vitro) and antiallergy effects of rice bran extracts. J Korean Soc Food Sci Nutr 35: 1297-1303. https://doi.org/10.3746/jkfn.2006.35.10.1297
  11. Lee EJ, Yang SA, Choi HD, Im HG, Whang K, Lee IS. 2011. Comparison of gingerols in various fractions and the antioxidant effects of supercritical fluid extracts from ginger. Korean J Food Sci Technol 43: 469-474. https://doi.org/10.9721/KJFST.2011.43.4.469
  12. Lee SM, Lee HB, Lee J. 2006. Comparison of extraction methods for the determination of vitamin E in some grains. J Korean Soc Food Sci Nutr 35: 248-253. https://doi.org/10.3746/jkfn.2006.35.2.248
  13. Kim JE, Lee S, Yoo KM, Lee KH, Kim KT, Lee MH, Hwang IK. 2014. Quality characteristics of ginseng seed oil obtained by different extraction methods. J Korean Soc Food Sci Nutr 43: 439-445. https://doi.org/10.3746/jkfn.2014.43.3.439
  14. Hedrick JL, Mulcahey LJ, Taylor LT. 1992. Supercritical fluid extraction. Microchimica Acta 108: 115-132. https://doi.org/10.1007/BF01242421
  15. Lee JH, Oh SK, Kim DJ, Yoon MR, Chun A, Choi IS, Lee JS, Kim YG. 2013. Comparison of antioxidant activities by different extraction temperatures of some commercially available cultivars of rice bran in Korea. Korean J Food Nutr 26: 1-7. https://doi.org/10.9799/ksfan.2013.26.1.001
  16. Minhajuddin M, Beg ZH, Iqbal J. 2005. Hypolipidemic and antioxidant properties of tocotrienol rich fraction isolated from rice bran oil in experimentally induced hyperlipidemic rats. Food Chem Toxicol 43: 747-753. https://doi.org/10.1016/j.fct.2005.01.015
  17. Jung EH, Hwang IK, Ha TY. 2010. Properties and antioxidative activities of phenolic acid concentrates of rice bran. Korean J Food Sci Technol 42: 593-597.
  18. Ham H, Yoon SW, Kim IH, Kwak J, Lee JS, Jeong HS, Lee J. 2015. Protective effects of unsaponifiable matter from rice bran on oxidative damage by modulating antioxidant enzyme activities in HepG2 cells. LWT-Food Sci Technol 61: 602-608. https://doi.org/10.1016/j.lwt.2014.12.047
  19. Kim I, Yoon SW, Lee J, Lee JS, Kim IH. 2013. Concentration of rice bran lipid soluble bioactive substances using supercritical carbon dioxide. Food Eng Prog 17: 362-368. https://doi.org/10.13050/foodengprog.2013.17.4.362
  20. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  21. Sharma OP, Bhat TK. 2009. DPPH antioxidant assay revisited. Food Chem 113: 1202-1205. https://doi.org/10.1016/j.foodchem.2008.08.008
  22. Oyaizu M. 1986. Studies on products of browning reaction: Antioxidative activity of products of browning reaction prepared from glucosamine. Jpn J Nutr 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  23. Elzaawely AA, Xuan TD, Tawata S. 2005. Antioxidant and antibacterial activities of Rumex japonicus HOUTT. aerial parts. Biol Pharm Bull 28: 2225-2230. https://doi.org/10.1248/bpb.28.2225
  24. Cicero AFG, Derosa G. 2005. Rice bran and its main components: Potential role in the management of coronary risk factors. Curr Top Nutraceutical Res 3: 29-46.
  25. Lai P, Li KY, Lu S, Chen HH. 2009. Phytochemicals and antioxidant properties of solvent extracts from Japonica rice bran. Food Chem 117: 538-544. https://doi.org/10.1016/j.foodchem.2009.04.031
  26. Chotimarkorn C, Benjakul S, Silalai N. 2008. Antioxidant components and properties of five long-grained rice bran extracts from commercial available cultivars in Thailand. Food Chem 111: 636-641. https://doi.org/10.1016/j.foodchem.2008.04.031
  27. Lee JW, Lee SW, Kim MK, Rhee C, Kim IH, Lee KW. 2005. Beneficial effect of the unsaponifiable matter from rice bran on oxidative stress in vitro compared with ${\alpha}$-tocopherol. J Sci Food Agric 85: 493-498. https://doi.org/10.1002/jsfa.2009

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