Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Combined Treatment of Acorn Pomace Extract, Fumaric Acid, and Mild Heat for Inactivation of Microorganisms on Red Chard

도토리박 추출물과 푸마르산 및 중온 열 병합처리에 의한 적근대의 미생물 제어 효과

  • Park, Shin-Min (Department of Food Science and Technology, Chungnam National University) ;
  • Son, Kyung Bin (Department of Food Science and Technology, Chungnam National University)
  • Received : 2016.07.05
  • Accepted : 2016.09.28
  • Published : 2016.11.30
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Abstract

In this study, acorn pomace extract (APE) was developed as a natural chemical sanitizer and substitute for chlorine-based sanitizers such as sodium hypochlorite containing harmful substances. Antimicrobial activities of APE and its combined treatments with fumaric acid (FA) and mild heat against Listeria monocytogenes inoculated on red chard were examined. Among the treatments, combined treatment of 0.5% APE at $50^{\circ}C$ and 0.5% FA was the most effective, causing reduction of L. monocytogenes populations by 3.36 log CFU/g compared to the control. After combined treatment, populations of aerobic mesophilic bacteria in the red chard decreased by 2.89 log CFU/g during storage at $4^{\circ}C$ for 8 days compared to the control. Regarding color changes in red chard upon combined treatment, there was no significant change among the red chard samples. These results indicate that combined treatment of APE, FA, and mild heat can improve microbial safety of red chard without affecting quality such as color during storage.

본 연구에서는 차아염소산 나트륨 같은 염소계 살균세척제를 대체하기 위한 천연 살균세척제로서 도토리박 추출물(APE)을 개발하기 위해 적근대에 Listeria monocytogenes를 접종한 후 APE의 항균 효과를 측정하였다. 그리고 최적 세척처리 조건을 수립하고자 APE, FA, $50^{\circ}C$에서의 mild heat를 병합처리하였다. 모든 처리구 중 0.5% APE/$50^{\circ}C$ mild heat/0.5% fumaric acid(FA) 병합 세척처리가 가장 효과적이었는데, L. monocytogenes 수를 대조구에 비해 3.36 log CFU/g 감소시켰다. 병합처리된 적근대 시료의 저장 8일 후 총 호기성균은 대조구와 비교하였을 때 2.89 log CFU/g 감소하였고, 색도 측정 결과 처리구 간 유의적인 차이가 나타나지 않았다. 따라서 본 연구 결과 APE/mild heat/FA 병합처리 방법은 수확 후 적근대의 품질에 영향을 미치지 않고 미생물학적 안전성을 높일 수 있는 효과적인 기술이라고 판단된다.

Keywords

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