The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지
DOI QR코드

DOI QR Code

Therapeutic and Prophylactic Effects of Zostera Marina on Dextran Sulfate Sodium-induced Colitis

해대(海帶) 추출물이 Dextran Sulfate Sodium로 유발된 대장염 동물모델에 미치는 치료 및 예방적 효과

  • Jeon, Woo-Hyeon (Department of Clinical Korean Medicine, Graduate School, Kyung Hee University) ;
  • Ko, Seok-Jae (Department of Gastroenterology, College of Korean Medicine, Kyung Hee University) ;
  • Ryu, Bongha (Department of Clinical Korean Medicine, Graduate School, Kyung Hee University) ;
  • Park, Jae-Woo (Department of Clinical Korean Medicine, Graduate School, Kyung Hee University)
  • 전우현 (경희대학교 대학원 임상한의학과) ;
  • 고석재 (경희대학교 한의과대학 비계내과학교실) ;
  • 류봉하 (경희대학교 대학원 임상한의학과) ;
  • 박재우 (경희대학교 대학원 임상한의학과)
  • Received : 2016.07.15
  • Accepted : 2016.09.06
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: Inflammatory bowel disease (IBD) is chronic inflammatory disorders of the intestines. Due to limitation of conventional treatment including steroids, herbal medicines have emerged as possible therapeutic options for IBD. The purpose of the current study was to investigate the therapeutic and prophylactic effects and mechanisms of Zostera Marina water extract (ZME) on DSS-induced colitis. Methods: Colitis was induced by DSS in Balb/c mice. In pre-treatment setting, ZME was administered 7 days before DSS treatment and in co-treatment setting, ZME was simultaneously administrated with DSS treatment. In both settings, ZME 100, 300 and 1000 mg/kg were orally administered twice a day, respectively. Mice weight and clinical findings were measured daily. Colon length, macroscopic findings and histological damages of colon mucosa were assessed at the end of experiments. The levels of cytokines including TNF-${\alpha}$, IFN-${\gamma}$, IL-$1{\beta}$, IL-6, IL-10 and IL-17 were measured by Biometric Multiplex Cytokine Profiling method. Results: In a dose dependent manner, ZME significantly inhibited the colon shortening, and improved macroscopic score and histological score. However, there were insignificant changes on inhibition of weight loss and improvement of clinical score. There were no significant differences of effects between co-treatment and pre-treatment settings. ZME 300 and 1000 mg/kg groups significantly inhibited IFN-${\gamma}$. Only ZME 1000 mg/kg group significantly inhibited TNF-${\alpha}$, IL-$1{\beta}$ and IL-6. Conclusions: The current results show the possibility of therapeutic use and its prophylactic application of ZME on inflammatory bowel diseases. Future studies for targeted mechanisms of ZME are needed.

Keywords

References

  1. Yang SK. Current Status and Clinical Characteristics of Inflammatory Bowel Disease in Korea. Korean J Gastroenterol. 2002;40:1-14.
  2. Department of Internal Medicine, Medical School, Seoul National University. Guideline of Internal Medicine. 1st ed. Seoul: Korea Medicine Publication. 2002:51-3.
  3. Kim JY. Gastrointestinal Disorders. 1st ed. Seoul: Iljogak. 2000:258-76.
  4. Park JW, Bu Y, Bae J, Lee B, Ko S, Kim J, et al. Protective effects of Ulmus macrocarpa on experimental colitis mice models. Orient Pharm Exp Med. 2011;11:107-12. https://doi.org/10.1007/s13596-011-0011-9
  5. Seo GS, Choi SC. Special Review-Ulcerative colitis: Pathophysiology of ulcerative colitis - Relationship with genetics and immunity-. Korean J Med. 2009;76(7):643-8.
  6. Kim TH, Kim BG, Sin HD, Kim JW, Kim CG, Kim JS, et al. Alimentary Tract: Tumor Necrosis Factor-a and Interleukin-10 Gen Polymorphisms in Korean Patients with Inflammatory Bowel Disease. Korean J Gastroenterol. 2003;42:377-86.
  7. Korean Association of Gastroenterology. Inflammatory Bowel Diseases. 1st ed. Seoul: Gunja Publication. 1999:61.
  8. Siegal CA. Review article: explaining risks of inflammatory bowel disease therapy to patients. Aliment Pharmacol Ther. 2011;33:23-32. https://doi.org/10.1111/j.1365-2036.2010.04489.x
  9. Lee JY, Kang HS, Park BE, Moon HJ, Sim SS, Kim CJ. Inhibitory effects of Geijigajakyak -Tang on trinitrobenzene sulfonic acid-induced colitis. J Ethnopharmacol. 2009;126:244-51. https://doi.org/10.1016/j.jep.2009.08.035
  10. Kim SY, Ryu B, Park JW. Effects of Samiunkyungtang on inflammation and fecal enzymes in ulcerative colitis animal model. J Korean Med. 2008;29(3):56-62.
  11. Ryu B, Ro W, Park JW, Bu Y, Lee BJ, Lim S, et al. Bojanggunbi-tang, a traditional Korean herbal prescription, ameliorates colonic inflammation induced by dextran sulfate sodium and 2,4,6-trinitrobenzene sulfonic acid in mice. J Ethnopharmacol. 2011;135(2):582-5. https://doi.org/10.1016/j.jep.2011.03.004
  12. Song YG, Ryu B, Yoon SW. The Effects of Soyumjungjang-tang on DSS-Induced Ulcerative Colitis in Mouse. Korean J Orient Int Med. 2008;29(2):385-400.
  13. Hong SS, Ryu B, Yoon SW, Kim JS. The effect of Sagunja-tang on TNBS-induced Inflammatory Bowel Disease in Mouse. Korean J Orient Int Med. 2010;31(4):731-51.
  14. Lee SW, Ryu B, Park JW. Effects of Sagassum pallidum on 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis in Mice. Korean J Orient Int Med. 2010;31(2):224-41.
  15. Chung HL, Yue GGL, To KF, Su YL, Huang Y, Ko WH. Effect of Scutellariae Radix extract on experimental dextran-sulfate sodium-induced colitis in rats. World J Gastroenterol. 2007;13(42):5605-11. https://doi.org/10.3748/wjg.v13.i42.5605
  16. Choi HG, Lee JH, Park HH, Sayeghet FAQ. Antioxidant and Antimicrobial Activity of Zostera marina L. Extract. Algae. 2009;24(3):179-84. https://doi.org/10.4490/ALGAE.2009.24.3.179
  17. Harrison PG. Control of microbial growth and of amphipod grazing by water-soluble compounds from leaves of Zostera marina. Mar Biol. 1982;67:225-30. https://doi.org/10.1007/BF00401288
  18. Harrison PG, Chan AT. Inhibition of the growth of micro-algae and bacteria by extracts of eelgrass(Zostera marina) leaves. Mar Biol. 1980;61:21-6. https://doi.org/10.1007/BF00410338
  19. Khasina EI, Kolenchenko EA, Sgrebneva MN, Kovalev VV, Khotimchenko YS. Antioxidant activities of a low etherfied pectin from the seagrass Zostera marina. Russian J Mar Biol. 2003;29:259-61. https://doi.org/10.1023/A:1025493128327
  20. Kolenchenko EA, Sonia LN, Khotimchenko YS. Comparative in vitro assessment of antioxidant activities of a low etherfied pectin from the eelgrass Zostera marina. and antioxidantive medicines. Russian J Mar Biol. 2005;31:331-4. https://doi.org/10.1007/s11179-005-0098-2
  21. Khasina EI, Tiupeleev PA, Sgrebneva MN. Gastroprotective effect of zosterin, a pectin from seagrass Zostera Marina L. Orient Pharm Exp Med. 2004;4(4):253-60. https://doi.org/10.3742/OPEM.2004.4.4.253
  22. Lee IA, Bae EA, Hyun YJ, Kim DH. Dextran sulfate sodium and 2,4,6-trinitrobenzene sulfonic acid induce lipid peroxidation by the proliferation of intestinal gram-negative bacteria in mice. J Inflamm. (Lond). 2010;7:7. https://doi.org/10.1186/1476-9255-7-7
  23. Kitajima S, Takoma S, Morimoto M. Histological analysis of murine colitis induced by dextran sulfate sodium of different molecular weights. Exp Animal. 2000;49:9-15. https://doi.org/10.1538/expanim.49.9
  24. Song MY, Park SY, Kim JH, Ahn SH, Kim KS, Sohn IC. Effects of Moxi-tar Herbal Acupuncture of LI11 on inflammatory bowel disease induced by TNBS in mice. Korean J Acupunct. 2008;25(3):147-66.
  25. Baek DB, Kwon OS, Choi WJ, Kim JH, Jeon HY, Kim KS, et al. Moxi-tar Herbal Acupuncture of BL25 Acupoint Ameliorates TNBS-Induced Colitis in Mice. Korean J Acupunct. 2007;24(3):149-64.
  26. Kim YT, Ahn SH, Kim JH, Sohn IC. Effects of Moxi-tar Herbal Acupuncture at Cheonchu (ST25) on Crohn's Disease Induced by TNBS in Mices. Korean J Acupunct. 2008;25(2):159-77.
  27. Kim SH, Kim HJ, Kim JE, Lee SH, Hong SH, Kim WI. Clinical Case of Symptoms Remaining after Western Medical Therapy in Ulcerative Colitis, with Herbal Medicine Dansamboheol-tang gagam, Acupuncture, and Moxibustion Treatment. Korean J Orient Int Med. 2007;28(4):911-8.
  28. Hwang JW, Choi HJ, Baik YS, Jeong SH, Shin GC, Lee WC. A Case of Pyungwijiyutang-gamibang Diagnosed as Constipation due to Stagnation of Eum with Ulcerative Colitis. Korean J Oriental Int Med. 2007;28(4):972-7.
  29. Kim CM, Shin MK, Ahn DG, Lee KS. The Great Dictionary of Chinese Herbs (Korean version). 1st ed. Seoul:Jungdam Publication. 1998:6036-7.
  30. Kirsner JB. Experimental colitis with particular reference to hypersensitivity reactions in the colon. Gastroenterology 1961;40:317-22.
  31. Okasu I, Hatakeyama S, Yamada M, Ohkusa T, Inagaki Y, Nakaya R. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology. 1990;98:694-702. https://doi.org/10.1016/0016-5085(90)90290-H
  32. MacPherson BR, Pfeiffer CJ. Experimental production of diffuse colitis in rat. Digestion. 1978;17:135-50. https://doi.org/10.1159/000198104
  33. Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, Wallace JL. Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology. 1989;96:795-803. https://doi.org/10.1016/S0016-5085(89)80079-4
  34. StroberW, Fuss IJ, Blumberg RS. The immunology of mucosal models of inflammation. Annu Rev Immunol. 2002;20:495-549. https://doi.org/10.1146/annurev.immunol.20.100301.064816
  35. Egger B, Bajaj-Elliott M, MacDonald TT, Inglin R, Eysselein VE, Buchler MW. Characterisation of acute murine dextran sodium sulphate colitis: cytokine profile and dose dependency. Digestion. 2000;62:240-8. https://doi.org/10.1159/000007822
  36. Ohkusa T. Production of experimental ulcerative colitis in hamsters by dextran sulfate sodium and change in intestinal microflora. Jpn J Gastroenterol. 1985;82:1327-36.
  37. Kitajima S, Takoma S, Morimoto M. Chages in colonic mucosal permeability mouse colitis induced with dextran sulfate sodium. Exp Animal. 1999;48:137-43. https://doi.org/10.1538/expanim.48.137
  38. Kim SJ, Choi DH, Chung YT. Gliotoxin protects against TNBS-induced colitis. via down-regulation of NF-kB activation. Kor J Anat. 2004;37(3):309-15.
  39. Johswich K, Martin M, Bleich A, Kracht M, Dittrich-Breiholz O, Gessner JE, et al. Role of the C5a receptor (C5aR) in acute and chronic dextran sulfate-induced models of inflammatory bowel disease. Inflamm Bowel Dis. 2009;15:1812-23. https://doi.org/10.1002/ibd.21012
  40. Ko E, Kim JG, Kim JR, Kim HJ, Hong SJ, Park JS, et al. Pathophysiology. 1st ed. Seoul: Korea Medicine Publication. 2006:42-3.
  41. O'Shea JJ, Murray PJ. Cytokine signaling modules in inflammatory responses. Immunity. 2008;28:477-87. https://doi.org/10.1016/j.immuni.2008.03.002
  42. Pizarro TT, Cominelli F. Cytokine therapy for Crohn's disease: advances in translational research. Annu Rev Med. 2007;58:433-44. https://doi.org/10.1146/annurev.med.58.121205.100607
  43. Dionne S, Hiscott J, D'Agata I, Duhaime A, Seidman EG. Quantitative PCR analysis of TNF-alpha and IL-1 beta mRNA levels in pediatric IBD mucosal biopsies. Dig Dis Sci. 1997;42:1557-66. https://doi.org/10.1023/A:1018895500721
  44. Targan SR, Hanauer SB, Van Deventer SJ, Mayer L, Present DH, Braakman T, et al. A short-term study of chimeric monoclonal antibody cA2 to tumor necrosis factor $\alpha$ for Crohn's disease. N Engl J Med. 1997;337:1029-35. https://doi.org/10.1056/NEJM199710093371502
  45. Kwon HH, Kim EJ, Kim IT, Park KH, Yang MG, Eum YB, et al. Clinical Immunology. 1st ed. Seoul: Korean Medicine Publication. 2010:158-70.
  46. Barnes PJ, Karin M. Nuclear factor-KB-a pivotal transcription factor in chronic inflammatory disease. N Engl J Med. 1997;336:1066-71. https://doi.org/10.1056/NEJM199704103361506
  47. Fedorak RN, Gangl A, Elson CO, Rutgeerts P, Schreiber S, Wild G, et al. Recombinant human interleukin 10 in the treatment of patients with mild to moderately active Crohn's disease. Gastroenterology. 2000; 119:1473-82. https://doi.org/10.1053/gast.2000.20229
  48. Schreiber S, Fedorak RN, Nielsen OH, Wild G, Williams CN, Nikolaus S, et al. Safety and efficacy of recombinant human interleukin-10 in chronic active Crohn's disease. Gastroenterology. 2000;119:1461-72 https://doi.org/10.1053/gast.2000.20196
  49. Fujino S, Andoh A, Bamba S, Ogawa A, Hata K, Araki Y, et al. Increased expression of interleukin 17 in inflammatory bowel disease. Gut. 2003;52:65-70. https://doi.org/10.1136/gut.52.1.65

Cited by

  1. Effect of dietary probiotics on colon length in an inflammatory bowel disease–induced murine model: A meta-analysis vol.103, pp.2, 2016, https://doi.org/10.3168/jds.2019-17356