Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Green Tea Polyphenol and Vitamin C on Type 2 Diabetic Rats Induced by Low Dose Streptozotocin Following High Fat Diet

고지방식이와 저용량 스트렙토조토신으로 유도된 2형 당뇨병 흰쥐에서 녹차 폴리페놀과 비타민 C 병합 투여 효과

  • Lee, Byoung-Rai (Department of Molecular Biology & Biochemistry, College of Medicine Chosun University) ;
  • Yang, Hoon (Department of Molecular Biology & Biochemistry, College of Medicine Chosun University) ;
  • Park, Pyoung-Sim (Department of Food Nutrient and Culinary, Chosun College of Science & Technology)
  • 이병래 (조선대학교 의과대학 생화학분자생물학교실) ;
  • 양훈 (조선대학교 의과대학 생화학분자생물학교실) ;
  • 박평심 (조선이공대학교 식품영양조리과학과)
  • Received : 2015.09.30
  • Accepted : 2015.11.23
  • Published : 2016.02.29
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Abstract

This study investigated the effects of green tea polyphenol and vitamin C on type 2 diabetes mellitus by administering polyphenon 60 (P60) and sodium ascorbate (SA) to diabetic rats induced by high fat diet/low-dose streptozotocin. The experimental group was divided into five different groups: non-diabetic control group (NC), diabetes control group (DC), diabetes+P60 group (DM+P60), diabetes+SA group (DM+SA), and diabetes+P60+SA group (DM+P60+SA). P60 and SA were dissolved in 0.1% drinking water. After P60 and SA were administered for 16 weeks, fasting blood glucose, plasma insulin, serum triglyceride, blood urea nitrogen (BUN), and creatinine levels as well as kidney alkaline phosphatase (AP) and ${\gamma}$-glutamyltranspeptidase (GGT) activities were measured. Fasting blood glucose level increased 5-fold in the DC group compared to the NC group. In the DM+P60 group, fasting blood glucose level decreased by 14%. In the DM+P60+SA group, fasting blood glucose level decreased by 28% compared to the DC group, whereas the DM+SA group did not show any significant difference. The homeostasis model assessment for insulin resistance index increased in the DC group and decreased in the DM+P60+SA group compared to the DC group. Serum creatinine level increased in the DC group, but decreased by 17% in the DM+P60 group and by 43% in the DM+P60+SA group compared to the DC group. The serum BUN level increased in the DC group, but decreased by 41% in the DM+P60+SA group compared to the DC group. Kidney GGT and AP activities decreased in the DC group compared to the NC group; however, they were reversed by DM+P60+SA group. These results show that combined administration of both green tea polyphenol and vitamin C had better effects on improving blood glucose level, insulin resistance, serum triglyceride level, and protecting kidneys than administration of either green tea polyphenol or vitamin C alone in the context of type 2 diabetes.

녹차 폴리페놀과 비타민 C가 2형 당뇨병에 미치는 영향을 관찰하기 위해 고지방식이와 저용량 스트렙토조토신을 이용하여 당뇨병이 유도된 흰쥐에 polyphenon 60(P60)과 sodium ascorbate(SA)를 16주간 투여한 후 공복 혈당, 혈장 인슐린, 혈청 중성지질, BUN 및 creatinine과 신장 GGT와 AP 활성도를 측정하였다. 당뇨병 흰쥐에 P60이나 SA 투여로 인한 식이섭취량과 체중의 변화는 없었다. 공복 혈당은 당뇨 대조군(DC 군)에서 대조군(NC 군)의 5배로 증가하였으며, 당뇨+녹차 폴리페놀(DM+P60) 군은 14%, 당뇨+녹차 폴리페놀+비타민 C(DM+P60+SA) 군은 28%가 DC 군보다 감소하였고, 당뇨+비타민 C(DM+SA) 군은 DC 군과 유의한 차이가 없었다. 혈장 인슐린 양은 각 실험군 사이에 유의한 차이가 없으며, 인슐린 저항성 지수 HOMA-IR은 DC 군에서 증가하였고 DM+P60+SA 군은 DC 군보다 감소하였다. 혈청 creatinine 양은 DC 군에서 증가하였고 DM+P60 군은 DC 군보다 17% 감소하였으며, DM+P60+SA 군은 DC 군보다 43% 감소하였다. 혈청 BUN 양은 DC 군에서 증가하였고 DM+P60+SA 군은 DC 군보다 41% 감소하였다. 신장 GGT와 AP 활성도는 DC 군에서 감소하였고 DM+P60+SA 군은 DC 군보다 감소가 적었으며, DM+SA 군은 DC 군과 유의한 차이가 없었다. 이상의 실험 결과 2형 당뇨병에서 녹차 폴리페놀과 비타민 C 복합 투여가 혈당, 인슐린 저항성 및 혈청 중성지질량 개선 및 신장 손상 방지효과가 녹차 폴리페놀이나 비타민 C를 단독으로 투여하였을 때보다 더 증가함을 확인할 수 있었다. 녹차 폴리페놀과 비타민 C의 혈당 상승 억제와 신장 손상 방지 효과에 대한 계속된 연구가 필요하며, 녹차 폴리페놀과 비타민 C는 당뇨병 환자에게 혈당을 조절하고 신장 손상을 방지하는 기능성 식품 제재로서 개발 가능성이 있는 것으로 생각한다.

Keywords

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