Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidative Effects of Extracts from Different Parts of Epimedium koreanum Nakai

삼지구엽초의 부위별 추출물에 따른 항산화 활성

  • Lee, Sung-hyun (Plant Resources Research Institute, Duksung Women's University) ;
  • Jang, Miran (Plant Resources Research Institute, Duksung Women's University) ;
  • Kim, Gun-Hee (Plant Resources Research Institute, Duksung Women's University)
  • 이성현 (덕성여자대학교 식물자원연구소) ;
  • 장미란 (덕성여자대학교 식물자원연구소) ;
  • 김건희 (덕성여자대학교 식물자원연구소)
  • Received : 2015.09.14
  • Accepted : 2016.01.06
  • Published : 2016.02.29
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Abstract

This study was conducted to investigate the antioxidant activities of 70% ethanol extracts from different parts (root, stem, and leaf) of Epimedium koreanum Nakai. Ethanol extracts from the three parts of Epimedium koreanum Nakai were assessed for total phenolic content, total flavonoid content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, myoglobin protective ratio, and ferric reducing antioxidant power (FRAP). Root part showed the highest total phenolic content ($2.468{\pm}0.017mg$ chlorogenic acid equivalent/g) and total flavonoid content ($2.071{\pm}0.002mg$ quercetin equivalent/g). Leaf part showed the strongest radical scavenging activities (DPPH radical; 80%, myoglobin protective ratio; 90% and FRAP; 98.7% at 20 mg/mL). Root part showed the highest ABTS radical scavenging at 1 mg/mL. Therefore, the results of the antioxidant activity test were correlated with total phenolic and flavonoid content values. Thus, Epimedium koreanum Nakai has great potential as a natural source for human health.

본 연구에서는 삼지구엽초의 뿌리, 줄기, 잎의 부위별 항산화 활성을 알아보기 위하여 각 부위를 70% 에탄올 용액을 이용하여 얻은 추출물의 총페놀 함량, 총플라보노이드 함량, DPPH 라디칼 소거 활성, ABTS 라디칼 소거 활성, 미오글로빈 보호 효과, FRAP를 추출 농도별(1, 2, 10, 20 mg/mL)로 측정하였다. 부위별 총페놀 함량의 경우 뿌리(2.468 mg), 잎(2.307 mg), 줄기(2.277 mg) 순으로 함량이 높았으며, 총플라보노이드 함량은 뿌리(2.071 mg), 잎(1.875 mg), 줄기(0.616 mg)로 순으로 측정되었다. DPPH 라디칼 소거활성, 미오글로빈 보호 효과 그리고 FRAP 실험에서는 농도 의존적으로 항산화 활성을 보였으며 플라보노이드 및 페놀성 화합물의 함량이 적은 줄기에 비하여 잎과 뿌리의 추출물의 활성이 좋았고 ABTS 라디칼 소거 활성의 경우 뿌리 추출물의 활성이 가장 높았다. 이는 총페놀 함량과 총플라보노이드의 함량이 항산화 활성에 상관관계가 있음을 확인할 수 있었다. 삼지구엽초는 주로 잎 부분이 식용으로 쓰이고 있으며 최근 지상부만을 제한적으로 식품원료로 허용하고 있는데 본 연구를 통하여 지상부 외에 지하부에서도 항산화 활성이 높은 것으로 확인되었다.

Keywords

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