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Inhibitory Effects of Transglycoslyation Products of Soy Isoflavones on Cholesterol Biosynthesis

대두 이소플라본 당전이 반응 산물의 콜레스테롤 생합성 저해 효과

  • Yoo, Lang Kuk (Department of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Seung Jun (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Moon, Tae Wha (Department of Agricultural Biotechnology, Seoul National University) ;
  • Shim, Jae-Hoon (Department of Food Science and Nutrition, Hallym University)
  • 유랑국 (서울대학교 농생명공학부) ;
  • 최승준 (서울과학기술대학교 식품공학과) ;
  • 문태화 (서울대학교 농생명공학부) ;
  • 심재훈 (한림대학교 식품영양학과)
  • Received : 2015.08.13
  • Accepted : 2015.09.25
  • Published : 2016.02.29

Abstract

Hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) is the rate-limiting enzyme in biosynthesis of cholesterol in animals. In this study, inhibitory effects of isoflavone glycosides on HMG-CoA reductase were investigated. At sample concentration of $100{\mu}M$, genistein-7-O-triglucoside (G2-genistin) inhibited HMG-CoA reductase activity by approximately 18%, whereas daidzein-7-O-triglucoside had no inhibitory effect. In the kinetic experiments with Syrian hamster HMG-CoA reductase, G2-genistin showed inhibitory efficacy with an invariable $V_{max}$ value, suggesting that G2-genistin works as a competitive inhibitor of HMG-CoA reductase and has potential for hypocholesterolemic action through direct regulation of HMG-CoA reductase.

HMG-CoA reductase는 체내 콜레스테롤 생합성에 있어서 속도제한단계(율속) 효소이다. 본 연구는 HMG-CoA reductase에 대한 이소플라본 배당체의 저해 효과를 연구하였다. $100{\mu}M$의 농도에서 genistein-7-O-triglucoside(G2-genistin)는 HMG-CoA reductase 활성을 약 18% 정도 저해하였으나, daidzein-7-O-triglucoside는 저해 효과를 갖지 않았다. 시리아햄스터 HMG-CoA reductase의 반응속도 실험에서 G2-genistin은 농도에 관계없이 $V_{max}$의 저해 정도가 일정하였으며, 이것은 G2-genistin이 HMG-CoA reductase의 경쟁적 저해제로 작용함을 시사하고 HMG-CoA reductase의 활성을 직접적으로 저해함으로써 혈중 콜레스테롤 함량을 감소시킬 수 있음을 예상할 수 있다.

Keywords

References

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