Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Supplement of tauroursodeoxycholic acid in vitrification solution improves the development of mouse embryos

  • Lin, Tao (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Lee, Jae-Eun (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Shin, Hyun-Young (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Oqani, Reza (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kim, So-Yeon (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Jin, Dong-Il (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • Received : 2016.02.23
  • Accepted : 2016.07.27
  • Published : 2016.12.31
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Abstract

This study was performed to determine whether supplementation of tauroursodeoxycholic acid (TUDCA), an endoplasmic reticulum (ER) stress inhibitor, during vitrified cryopreservation enhances the development of frozen mouse embryos. Mouse 8-cell stage embryos were collected and exposed to a cryoprotectant solution containing TUDCA or TM (tunicamycin, an ER stress inhibitor) at room temperature and stored in liquid nitrogen following vitrification. The final concentration of TUDCA or TM was $50{\mu}M$. The survival and development rates of mouse 8-cell stage embryos exposed to TUDCA- or TM-containing solutions at room temperature or stored in liquid nitrogen following vitrification were measured. There were no significant differences in survival rate and blastocyst formation rate among control, TUDCA, and TM groups after embryos were exposed to vitrification solutions at RT. When mouse 8-cell stage embryos were treated with TUDCA or TM and then stored in liquid nitrogen, the survival rates of control and TUDCA groups were significantly higher than for the TM group. Blastocyst formation rate of the TUDCA group following in vitro culture was significantly higher than that in control or TM groups. The TM group showed a lower (p < 0.05) blastocyst formation rate than the other two groups. Our results indicate that TUDCA supplementation during cryopreservation of mouse embryos could enhance their development capacity.

Keywords

References

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