Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Pretreatment and enzymatic saccharification process of rapeseed straw for production of bioethanol

  • Lee, Heon-Hak (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Jeon, Min-Ki (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, Chungnam National University)
  • Received : 2016.11.30
  • Accepted : 2016.12.27
  • Published : 2016.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to evaluate the yield of bio-ethanol produced by separate hydrolysis and fermentation (SHF) with the pretreated rapeseed straw (RS) using crude enzyme of Cellulomonas flavigena and Saccharomyces cereviase. Crude enzyme of C. flavigena showed enzymatic activity of 14.02 U/mL for CMC 133.40 U/mL, for xylan 15.21 U/mL, for locust gum and 15.73 U/mL for rapeseed straw at pH 5.0 and $40^{\circ}C$, respectively. The hemicellulose contents of RS was estimated to compromise 36.62% of glucan, 43.20% of XMG (xylan + mannan + galactan), and 2.73% of arabinan by HPLC analysis. The recovering ratio of rapeseed straw were investigated to remain only glucan 75.2% after 1% $H_2SO_4$ pretreatment, glucan 45.44% and XMG 32.13% after NaOH, glucan 44.75% and XMG 5.47% after $NH_4OH$, and glucan 41.29% and XMG 41.04% after hot water. Glucan in the pretreatments of RS was saccharified to glucose of 45.42 - 64.81% by crude enzyme of C. flavigena while XMG was made into to xylose + mannose + galactose of 58.46 - 78.59%. Moreover, about 52.88 - 58.06 % of bio-ethanol were obtained from four kinds of saccharified solutions by SHF using S. cerevisiae. Furthermore, NaOH pretreatment was determined to show the highest mass balance, in which 21.22 g of bio-ethanol was produced from 100 g of RS. Conclusively, the utilization of NaOH pretreatment and crude enzyme of Cellulomonas flavigena was estimated to be the best efficient saccharification process for the production of bio-ethanol with rapeseed straw by SHF.

Keywords

References

  1. Abt B, Foster B, Lapidus A, Clum A, Sun H, Pukall R, Lucas S, Del Rio TG, Nolan M, Tice H, Cheng JF, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Ovchinnikova G, Pati A, Goodwin L, Chen A, Palaniappan K, Land M, Hauser L Chang YJ, Jeffries CD, Rohde M, Goker M, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP. 2010. Complete genome sequence of Cellulomonas flavigena type strain (134 T). Standards in Genomic Sciences 3:15-25. https://doi.org/10.4056/sigs.1012662
  2. Beg Q, Kapoor M, Mahajan L, Hoondal GS. 2001. Microbial xylanases and their industrial applications: a review. Applied Microbiology and Biotechnology 56:326-338. https://doi.org/10.1007/s002530100704
  3. Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntoon FM. 1923. Genus II. Flavobacterium gen. nov. Bergey's Manual of Determinative Bacteriology 97-117.
  4. Bradford, Marion M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72.1-2:248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  5. Dubos RJ. 1928. The decomposition of cellulose by aerobic bacteria. Journal of Bacteriology 15:223.
  6. Gwak IS, Hwang JH, Lee SH. 2016. Techno-economic evaluation of an ethanol production process for biomass waste. Applied Microbiology and Biotechnology 27:171-178. [in Korean]
  7. Han JG, Oh SH, Jeong MH, Kim SS, Seo HB, Jeong KH, Jang YS, Kim IC, Lee HY. 2009. Two-step high temperature pretreatment process for bioethanol production from rape stems. KSBB Journal 24:489-494. [in Korean]
  8. Humbird D, Davis R, Tao L, Kinchin C, Hsu D, Aden A, Schoen P, Lukas J, Olthof B, Sexton D, Dudgeon D. 2011. Process design and economics for biochemical conversion of lignocellulosic biomass to ethanol: Dilute-acid pretreatment and enzymatic hydrolysis of corn stover (No. NREL/TP-5100-47764). National Renewable Energy Laboratory (NREL), Golden, CO.
  9. Kamm B. 2007. Production of platform chemicals and synthesis gas from biomass. Angewandte Chemie International Edition 46:5056-5058. https://doi.org/10.1002/anie.200604514
  10. Kang K. E, Jeong GT, Sunwoo C, Park DH. 2012. Pretreatment of rapeseed straw by soaking in aqueous ammonia. Bioprocess and Biosystems Engineering 35:77-84. https://doi.org/10.1007/s00449-011-0606-z
  11. Lee AY, Koo JK. 2014. Intermediate indicators and sustainability of renewable fuel standard. Journal of the Korea Organic Resource Recycling Association 22:20-26. [in Korean]
  12. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31:426-428. https://doi.org/10.1021/ac60147a030
  13. Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M. 2005. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresource Technology 96:673-686. https://doi.org/10.1016/j.biortech.2004.06.025
  14. Nigam PS, Singh A. 2011. Production of liquid biofuels from renewable resources. Progress in Energy and Combustion Science 37:52-68. https://doi.org/10.1016/j.pecs.2010.01.003
  15. Perez-Avalos O, Ponce-Noyola T, Magana-Plaza I, de la Torre M. 1996. Induction of xylanase and ${\beta}$-xylosidase in Cellulomonas flavigena growing on different carbon sources. Applied Microbiology and Biotechnology 46:405-409.
  16. Rajoka MI. 2004. Influence of various fermentation variables on exo-glucanase production in Cellulomonas flavigena. Electronic Journal of Biotechnology 7:07-08.
  17. Shrestha RK, Hur O, Kim TH. 2013. Pretreatment of corn stover for improved enzymatic saccharification using ammonia circulation reactor (ACR). Korean Chemical Engineering Research 51:335-341. [in Korean] https://doi.org/10.9713/kcer.2013.51.3.335
  18. Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D. 2008. Determination of structural carbohydrates and lignin in biomass. Laboratory Analytical Procedure 1617.
  19. Talebnia F, Mighani M, Rahimnejad M, Angelidaki I. 2015. Ethanol production from steam exploded rapeseed straw and the process simulation using artificial neural networks. Biotechnology and Bioprocess Engineering 20:139-147. https://doi.org/10.1007/s12257-013-0535-6

Cited by

  1. Development of succinate producing Cellulomonas flavigena mutants with deleted succinate dehydrogenase gene vol.44, pp.1, 2016, https://doi.org/10.7744/kjoas.20170004
  2. Incubation of Scenedesmus quadricauda based on food waste compost vol.47, pp.4, 2016, https://doi.org/10.7744/kjoas.20200087