Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl4-Induced liver Injury in Mice

  • Chen, Ping (Department of Pharmacognosy, West China School of Pharmacy, Sichuan University) ;
  • Chen, Yang (Department of Pharmacognosy, West China School of Pharmacy, Sichuan University) ;
  • Wang, Yarong (Department of Pharmaceutical Sciences, Zunyi Medical University Zhuhai Campus) ;
  • Cai, Shining (Department of Pharmaceutical Sciences, Zunyi Medical University Zhuhai Campus) ;
  • Deng, Liang (Department of Pharmacognosy, West China School of Pharmacy, Sichuan University) ;
  • Liu, Jia (Department of English, Zunyi Medical University Zhuhai Campus) ;
  • Zhang, Hao (Department of Pharmacognosy, West China School of Pharmacy, Sichuan University)
  • Received : 2015.07.06
  • Accepted : 2015.12.29
  • Published : 2016.03.01
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Abstract

Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in Gardenia jasminoides Ellis. In the present study, geniposide, crocins, crocin-1 and crocetin were separated from gardenia chromatographically. Then, mice were orally administrated with geniposide (400 mg/kg b.w.), crocins (400 mg/kg b.w.), crocin-1 (400 mg/kg b.w.) and crocetin (140 mg/kg b.w.) once daily for 7 days with $CCl_4$. Hepatoprotective properties were evaluated by biochemical parameters: Administration of geniposide, crocins, crocin-1and crocetin significantly lowered serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels in $CCl_4$-treated mice. The reduced glutathione (GSH) levels and antioxidant enzymes (SOD and CAT) activities were also increased by geniposide, crocins, crocin-1 and crocetin. Histopathological examination of livers showed that these components reduced deformability, irregular arrangement and rupture of hepatocyte in $CCl_4$-treated mice. These biochemical results and liver histopathological assessment demonstrated that geniposide, crocetin derivatives and crocetin show comparative beneficial effects on $CCl_4$-induced liver damage via induction of antioxidant defense. Therefore, contents of geniposide and crocetin derivatives should be both considered for hepatoprotective efficacy of Gardenia jasminoides Ellis.

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References

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