The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY (한국해양학회지:바다)

The Korean Society of Oceanography (한국해양학회)
권호 표지
DOI QR코드

DOI QR Code

Use of Molecular Detection Technique for Red Tide Warning of Cochlodinium polykrikoides

Cochlodinium polykrikoides 적조출현주의보 발령에 분자탐침기법의 활용

  • PARK, TAE GYU (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • WON, KYOUNG MI (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • KIM, WON JIN (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS))
  • 박태규 (국립수산과학원 남동해수산연구소) ;
  • 원경미 (국립수산과학원 남동해수산연구소) ;
  • 김원진 (국립수산과학원 남동해수산연구소)
  • Received : 2015.10.02
  • Accepted : 2015.12.23
  • Published : 2016.02.29
Download PDF
⟨ Previous Next ⟩

Abstract

Real-time PCR was applied to early warning of red tide. For early warning of red tide at $10cells\;mL^{-1}$, Cochlodinium polykrikoides specific real-time PCR was used as a complement of microscopy that has a lower detection sensitivity. C. polykrikoides appeared extensively in Tongyeong, Namhae waters at low densities in the end of June, and early warning of C. polyrkrikoides blooms was issued on 2 August 2015.

Real-time PCR을 적조출현주의보 발령에 활용하였다. Cochlodinium polykrikoides 적조예보를 위해 $10cells\;mL^{-1}$ 이상에서 적조출현주의보를 발령하였는데 현미경 검경은 저밀도에서 정확도가 낮기 때문에 이를 보완하기 위해 C. polykrikoides 특이적 real-time PCR을 사용하였다. 6월 말경에 C. polykrikoides이 통영, 남해 해역에 저밀도로 광범위하게 출현하였고 2015년 8월 2일 적조출현주의보를 발령하였다.

Keywords

References

  1. Bower, H.A., Tengs, T., Glasgow, H.B., Burkholder, J.M., Rublee, P.A. and Oldach, D.W., 2000. Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates. Appl. Environ. Microbiol. 66: 4641-4648. https://doi.org/10.1128/AEM.66.11.4641-4648.2000
  2. Coyne, K., Handy, S.H., Demir, E., Whereat, E.B., Hutchins, D.A., Portune, K.J., Doblin, M.A. and Craig, C.S., 2005. Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard. Limnol. Oceanogr. Meth. 3: 381-391. https://doi.org/10.4319/lom.2005.3.381
  3. Handy, S.M., Demir, E., Hutchins, D.A., Portune, K.J., Whereat, E.B., Hare, C., Rose, J., Warner, M., Farestad, M. and Cary, S.C., 2008. Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies. Harmful Algae 7: 599-613. https://doi.org/10.1016/j.hal.2007.12.018
  4. Lee, C.K., Park, T.G., Park Y.T. and Lim, W.A. 2013. Monitoring and trends in red-tide events in Korea with emphasis on Cochlodinium polykrikoides. Harmful Algae 30S: 3-14.
  5. Park, T.G., de Salas, M.F., Bolch, C.J.S. and Hallegraeff, G.M., 2007. Development of a real-time PCR probe for quantification of the heterotrophic dinoflagellate Cryptoperidiniopsis brodyi (Dinophyceae) in environmental samples. Appl. Environ. Microbiol. 73: 2552-2560. https://doi.org/10.1128/AEM.02389-06
  6. Park, T.G., Park, G.H., Park, Y.T., Kang, Y.S., Bae, H.M., Kim, C.H., Jeong, H.J. and Lee, Y., 2009a. Identification of the dinoflagellate community during Cochlodinium polykrikoides (Dinophyceae) blooms using amplified rDNA melting curve analysis and realtime PCR probes. Harmful Algae 8: 430-440. https://doi.org/10.1016/j.hal.2008.09.003
  7. Park, T.G., Park, Y.T. and Lee Y., 2009b. Development of a SYTO9 based real-time PCR probe for detection and quantification of toxic dinoflagellate Karlodinium veneficum (Dinophyceae) in environmental samples. Phycologia 48: 32-43. https://doi.org/10.2216/08-52.1
  8. Park, T.G. and Park, Y.T., 2010. Detection of Cochlodinium polykrikoides and Gymnodinium impudicum (Dinophyceae) in sediment samples from Korea using real-time PCR. Harmful Algae 9: 59-65. https://doi.org/10.1016/j.hal.2009.08.002
  9. Park, T.G., Lim, W.A., Park, Y.T., Lee, C.K. and Jeong, H.J., 2013. Economic impact, management and mitigation of red tides in Korea. Harmful Algae 30S: 131-143.