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The effect of decylcyclopropene derivative on the softening of 'Fuyu' persimmon fruits

Decylcyclopropene 유도체가 부유 단감 과실의 연화에 미치는 영향

  • 최성진 (대구가톨릭대학교 생명공학과) ;
  • 안광환 (경남농업기술원 단감연구소)
  • Received : 2014.11.13
  • Accepted : 2015.11.16
  • Published : 2016.02.28

Abstract

In order to develop a sprayable ethylene antagonist, unlike 1-methylcyclopropene (MCP), we synthesized 2-decylcyclopropene-1-carboxylic acid ethyl ester (DCPE) as a derivative of cyclopropene and tested its effect on the flesh softening of 'Fuyu' persimmon fruits. The fruits on trees were sprayed with $4{\cdot}10^{-4}$ M DCPE solution before harvest. After harvest, the persimmon fruits were stored at a low temperature for 1.5 months. The ripening progress of the fruits was then evaluated during storage at ambient temperature ($20^{\circ}C$). Flesh softening, a measure of ethylene response, was considerably delayed up to 7 days after DCPE treatment. However control fruits was rapidly softened after 3-day storage. The treatment effect of DCPE at $4{\cdot}10^{-4}$ M was also compared to that of 1-MCP at 1 ppm. DCPE was storable at refrigerated conditions for at least one month without any loss. The results show that DCPE could be a potential sprayable agent for the prevention of flesh softening of persimmon fruit.

상온에서 기체로 존재하는 기존의 에틸렌 작용억제제인 1-MCP와 달리 상온에서 액상으로 존재하여 분무살포가 가능한 에틸렌 작용억제제를 개발할 목적으로 cyclopropene 유도체의 하나로서 2-decylcyclopropene-1-carboxylic acid ethyl ester(DCPE)를 합성하여 부유 단감을 대상으로 그 처리효과를 분석하였다. $4{\cdot}10^{-4}$ M의 DCPE를 수확 전에 분무살포 처리한 단감을 수확하여 45일간 저온저장한 후 상온($20^{\circ}C$)에 1주간 방치하면서 성숙 관련 지표의 변화를 조사한 결과, 에틸렌의 작용에 의해 유발되는 과육 연화의 진행이 효과적으로 지연되었으며, 그 억제 효과는 1 ppm의 1-MCP 처리와 대등한 수준이었다. 또한 DCPE는 적어도 한달간 성분 손실 없이 냉장 보관이 가능하였다. 따라서 DCPE는 단감 과실의 수확 후 과육의 연화를 지연시키는 약제로의 활용이 가능할 것으로 기대된다.

Keywords

References

  1. Abeles FB, Morgan PW, Saltveit ME (1992) Ethylene in plant biology. Academic Press, New York, USA
  2. Binder BM (2008) The ethylene receptors : complex perception for a simple gas. Plant Sci, 175, 8-17 https://doi.org/10.1016/j.plantsci.2007.12.001
  3. Solano R, Ecker JR (1998) Ethylene gas : perception, signalling and response. Cur Opinion Plant Biol, 1, 393-398 https://doi.org/10.1016/S1369-5266(98)80262-8
  4. Guo H, Ecker JR (2004) The ethylene signaling pathway : new insights. Cur Opinion Plant Biol, 7, 40-49 https://doi.org/10.1016/j.pbi.2003.11.011
  5. Hua J, Meyerowitz EM (1998) Ethylene responses are negatively regulated by a receptor gene family in Arabidopsis thaliana. Cell, 94, 261-271 https://doi.org/10.1016/S0092-8674(00)81425-7
  6. Sisler EC, Pian A (1973) Effect of ethylene and cyclic olefins on tobacco leaves. Tobacco Sci, 1, 68-72
  7. Sisler EC, Yang SF (1984) Anti-ethylene effects of cis-2-butene and cyclic olefins. Phytochem, 23, 2765-2768 https://doi.org/10.1016/0031-9422(84)83011-3
  8. Sisler EC (2006) The discovery and development of compounds counteracting ethylene at the receptor level. Biotechnol Advances, 24, 357-367 https://doi.org/10.1016/j.biotechadv.2006.01.002
  9. Choi SJ (2010) The change of ethylene production, respiration, and flesh firmness as influenced by treatment with aminoethoxyvinylglycine and 1-methycyclopropene in 'Fuyu' persimmon fruits stored at low temperature. Korean J Hort Sci Technol, 28, 254-258
  10. Lelievre JM, Latche A, Jones B, Bouzayen M, Pech JC (1997) Ethylene and fruit ripening. Physiol Plant, 101, 727-739 https://doi.org/10.1111/j.1399-3054.1997.tb01057.x
  11. Besada C, Jackman RC, Olsson S, Woolf AB (2010) Response of 'Fuyu' persimmons to ethylene exposure before and during storage. Postharvest Biol Technol, 57, 124-131 https://doi.org/10.1016/j.postharvbio.2010.03.002
  12. Itamura H, Kitamura T, Taira S, Harada H, Ito N, Takahashi Y, Fukushima T (1991) Relationship between fruit softening, ethylene production and respiration in Japanese persimmon 'Hiratanenashi'. J Japan Soc Hort Sci, 60, 695-701 https://doi.org/10.2503/jjshs.60.695
  13. Takata M (1981) Effects of silver ion on the ripening of Japanese persimmon fruits. J Japan Soc Hort Sci, 50, 372-378 https://doi.org/10.2503/jjshs.50.372
  14. Beyer EM (1976) A potent inhibitor of ethylene action in plants. Plant Physiol, 58, 268-271 https://doi.org/10.1104/pp.58.3.268
  15. Harima S, Nakano R, Yamauchi S, Kitano Y, Yamamoto Y, Inaba A, Kubo Y (2003) Extending shelf-life of astringent persimmon (Diospyros kaki Thunb.) fruit by 1-MCP. Postharvest Biol Technol, 29, 318-323
  16. Luo Z (2007) Effect of 1-methylcyclopropene on ripening of postharvest persimmon (Diospyros kaki L.) fruit. LWT-Food Sci Thechnol, 40, 285-291 https://doi.org/10.1016/j.lwt.2005.10.010
  17. Blankenship SM, Dole JM (2003) 1-Methylcyclopropene : a review. Postharvest Biol Technol, 28, 1-25 https://doi.org/10.1016/S0925-5214(02)00246-6
  18. Liao L, Zhang F, Yan N, Golen JA, Fox JM (2004) An efficient and general method for resolving cyclopropene carboxylic acids. Tetrahedron, 60, 1803-1816 https://doi.org/10.1016/j.tet.2003.12.042
  19. Still WC, Kahn M, Mitra A (1978) Rapid chromatographic technique for preparative separations with moderate resolution. J Org Chem, 43, 2923-2925 https://doi.org/10.1021/jo00408a041
  20. Schipperijn AJ, Smael P (1973) Chemistry of cyclopropene. II. Formation and reactions of 1-potassio, 1-sodio and 1-lithiocyclopropene in liquid ammonia. Recueil, 92, 1121-1166
  21. Sisler EC, Grichko VP, Serek M (2006) Interaction of ethylene and other compounds with the ethylene receptor : agonists and antagonists. Ethylene Action in Plants, Springer-Verlag, Berlin, p 1-34
  22. Fisher F, Applequist DE (1965) Synthesis of 1-methylcyclopropene. J Org Chem, 30, 2089-2090 https://doi.org/10.1021/jo01017a531