Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Hyaluronidase inhibitory activity of extracted phenolic compounds from ultrafine grind Saururus chinensis

초미세 분쇄한 삼백초로부터 추출된 phenolic 화합물의 hyaluronidase 억제 효과

  • Park, Ki-Tae (School of Culinary Art and Baking Technology, Dongju College University) ;
  • Oh, Sang-Lyong (School of Food Science and Biotechnology / Food and Bio-Industry Research Institute, Kyungpook National University) ;
  • Cho, Young-Je (School of Food Science and Biotechnology / Food and Bio-Industry Research Institute, Kyungpook National University)
  • 박기태 (동주대학교 외식조리제과계열) ;
  • 오상룡 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 조영제 (경북대학교 식품공학부/식품생물산업연구소)
  • Received : 2015.10.07
  • Accepted : 2015.12.02
  • Published : 2016.02.28
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Abstract

In this study, the anti-inflammatory effect of hyaluronidase (HAase) inhibition was determined from 92 species of oriental herbal medicine extracted with water and ethanol solvents because of their non-toxicity in the human body. The water extracts of Evodia officinalis (86.8%), Thuja orientalis (80.8%), Carthami semen (66.5%), Melia azedarach (74.7%), Siegesbeckia pubescens (61.3%), Saururus chinensis (49.15%) showed a relatively greater anti-inflammatory activity. The ethanol extracts of Ailanthus altissima and Saururus chinensis demonstrated the highest anti-inflammatory effect at above 90%. Saururus chinensis was selected for its high anti-inflammatory effect in both water and ethanol extract. Ethanol was more effective than water and optimal extraction conditions for phenolic compounds was determined to be extraction with 50% ethanol for 12 hours. The extracts from Saururus chinensis in optimal condition showed 70~80% anti-inflammatory effect when $100{\sim}250{\mu}g/mL$ phenolic concentration was treated. Concentration of above $500{\mu}g/mL$ decreased the inhibitory effect. The anti-inflammatory effect and extraction yield were increased by ultra-fine grind technology, indicating that this method can be used to increase the extraction yield of phenolic compounds from medicinal plants.

본 연구에서 92종의 한약재로부터 물과 ethanol을 용매추출물에 대하여 HAase의 저해에 의한 항염증 효과를 측정한 결과 물 추출물에서는 E. officinalis(86.8%), T. orientalis(80.8%), C. semen(66.5%), M. azedarach(74.7%), S. pubescen (61.3%), S. chinensis(49.15%) 등이 높게 나타났다. Ethanol 추출물에서는 A. altissima와 S. chinensis 추출물이 90% 이상의 높은 항염증 활성을 나타내었다. 이들 중 물과 에탄올 추출물에서 모두 항염증 활성이 높게 측정된 S. chinensis가 선발되었다. 선별된 삼백초의 유효성분을 보다 효율적으로 추출할 방안을 확립하기 위하여 인체에 유해하지 않은 용매로 물과 ethanol을 선택하여 추출 최적조건을 살펴보았다. Phenolic 성분의 추출을 위한 최적조건은 50% ethanol을 사용하여 12시간 추출이 최적이었다. Phenolic 성분의 추출을 위해서는 ethanol이 물보다 더 효율적이었다. 최적조건에서 추출된 삼백초 추출물의 염증억제 활성은 $100{\sim}250{\mu}g/mL$ phenolic 농도로 첨가했을 때 70~80%의 염증 억제 효과를 나타내었으나, $500{\mu}g/mL$ 이상의 농도로 첨가 시 오히려 염증 억제활성이 감소하는 경향을 나타내었다. 삼백초를 초미세 분쇄 하였을 때 추출 수율과 염증억제 활성이 증가하는 것을 확인하였다. 따라서 초미세 분쇄 기술은 한약재로부터 생리활성물질 추출수율의 증가를 목적으로 적용할 수 있는 기술로 개발이 가능할 것으로 판단되었다.

Keywords

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