Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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How to Get Well-Preserved Samples for Transmission Electron Microscopy

  • Park, Chang-Hyun (Medical Science Research Center, Korea University College of Medicine) ;
  • Kim, Hyun-Wook (Medical Science Research Center, Korea University College of Medicine) ;
  • Rhyu, Im Joo (Department of Anatomy, Korea University College of Medicine) ;
  • Uhm, Chang-Sub (Department of Anatomy, Korea University College of Medicine)
  • Received : 2016.12.01
  • Accepted : 2016.12.16
  • Published : 2016.12.30
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Abstract

Proper sample preparation prior to microscopy is necessary for maintaining the components of tissues in a state as close to a living state as possible. For optimal preservation of biological samples, the sampling conditions are as important as the fixation itself. Various factors influence the selection and fixation efficiencies of a fixative, including sample size, osmolarity, pH, penetration rate and depth, fixative temperature, fixation time, fixative concentration, fixative amount, and retention time. Therefore, several factors for selecting and administering fixation procedures are evaluated pertaining to optimal sample preparation for transmission electron microscopy.

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References

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