Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Anti-inflammatory Effects of Flavokavain C from Kava (Piper methysticum) Root in the LPS-induced Macrophages

LPS로 유도된 대식세포에서 카바뿌리로부터 분리한 Flavokavain C의 항염증 효과

  • Park, Chung (LINC Project Group, Division of Life Science, Daejeon University) ;
  • Han, Jong-Min (LINC Project Group, Division of Life Science, Daejeon University)
  • 박청 (대전대학교 LINC사업단, 생명과학과) ;
  • 한종민 (대전대학교 LINC사업단, 생명과학과)
  • Received : 2016.08.21
  • Accepted : 2016.11.17
  • Published : 2016.12.30
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Abstract

Kava (Piper methysticum, P. methysticum) is used as traditional herbal medicine for urogenital diseases, rheumatisms, gastrointestinal problems, respiratory irritations, and pulmonary pains. We identified a flavokavain C (FKC) from P. methysticum, which showed anti-inflammatory activity on nuclear factor ${\kappa}B$ (NF-${\kappa}B$)-dependent nitric oxide (NO) production and expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. FKC inhibited accumulation of reactive oxygen species (ROS), such as hydrogen peroxide, and was able to dose-dependently reduce the LPS-induced NO production and the expression of various inflammation-associated genes (iNOS, IL-$1{\beta}$, IL-6) through inhibition of NF-${\kappa}B$ and MAPKs (ERK and JNK). In conclusion, these results indicate that FKC may have the potential to prevent inflammation process including NF-${\kappa}B$ and MAPKs pathways, and it could be applicable to functional cosmetics for anti-inflammation and antioxidant properties.

카바(Piper methysticum, P. methysticum)는 비뇨생식기 질환, 류머티즘, 위장 장애, 호흡기 자극 및 폐 통증에 대해 전통적으로 사용되는 것으로 알려져 있다. 본 연구에서는 카바에서 분리된 flavokavain C (FKC)가 염증성 유전자의 발현에 관여하는 전사인자인 핵요소-${\kappa}B$ (NF-${\kappa}B$) 의존성 산화 질소(NO) 생산 및 산화 질소 합성 효소(iNOS)의 발현에 리포폴리사카라이드(LPS) 처리된 대식세포에서 항 염증 활성을 나타낸다는 것을 확인하였다. FKC는 과산화수소와 같은 반응성 산소 종(ROS)의 축적을 억제하고, LPS로 유도된 NO 생성 및 각종 염증 관련 유전자(iNOS, IL-$1{\beta}$, IL-6)의 발현을 NF-${\kappa}B$ 및 MAPKs (ERK 및 JNK)의 억제를 통해 농도 의존적으로 줄일 수 있었다. 결론적으로, 이러한 결과는 FKC가 NF-${\kappa}B$ 경로와 MAPKs 포함한 염증 프로세스를 억제하는 능력을 가지고 있음을 나타내며, 이는 항 염증 및 항산화 효능 기반 기능성 화장품에 적용될 수 있음을 암시한다.

Keywords

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