The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction

  • Kang, In Jeong (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kang, Mi-Hyung (Capacity Building Division, Rural Development Administration) ;
  • Noh, Tae-Hwan (Planning and Coordination Division, National Institute of Crop Science, Rural Development Administration) ;
  • Shim, Hyeong Kwon (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Shin, Dong Bum (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Heu, Suggi (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
  • Received : 2016.05.04
  • Accepted : 2016.07.29
  • Published : 2016.12.01
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Abstract

Burkholderia glumae (bacterial grain rot), Xanthomonas oryzae pv. oryzae (bacterial leaf blight), and Acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for A. avenae subsp. avenae and B. glumae, and transposase A gene sequence for X. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for B. glumae, 490 bp for X. oryzae, and 290 bp for A. avenae subsp. avenae with the $63^{\circ}C$ as an optimum annealing temperature. Samples collected from naturally infected fields were detected with two bacteria, B. glumae and A. avenae subsp. avenae but X. oryzae pv. oryzae was not detected. This assay can be used to identify pathogens directly from infected seeds, and will be an effective tool for the identification of the three pathogens in rice plants.

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References

  1. Asano, T., Senda, M., Suga, H. and Kageyama, K. 2010. Development of multiplex PCR to detect five Pythium species related to turfgrass diseases. J. Phytopathol. 158:609-615.
  2. Gitaitis, R. and Walcott, R. 2007. The epidemiology and management of seedborne bacterial diseases. Annu. Rev. Phytopathol. 45:371-397. https://doi.org/10.1146/annurev.phyto.45.062806.094321
  3. Goto, M. 1964. Nomenclature of the bacteria-causing bacterial leaf streak and bacterial stipe of rice. Bull. Fac. Agric. Shizuoka Univ. 14:3-10.
  4. Ham, J. H., Melanson, R. A. and Rush, M. C. 2011. Burkholderia glumae: next major pathogen of rice? Mol. Plant Pathol. 12:329-339. https://doi.org/10.1111/j.1364-3703.2010.00676.x
  5. James, D., Varga, A., Pallas, V. and Candresse, T. 2006. Strategies for simultaneous detection of multiple plant viruses. J. Plant Pathol. 28:16-29.
  6. Jeong, Y., Kim, J., Kim, S., Kang, Y., Nagamatsu, T. and Hwang, I. 2003. Toxoflavin produced by Burkholderia glumae causing rice grain rot is responsible for inducing bacterial wilt in many field crops. Plant Dis. 87:890-895. https://doi.org/10.1094/PDIS.2003.87.8.890
  7. Kadota, I. 1996. Studies on the pathogen of bacterial brown stripe of rice (Oryza sativa) and its ecology in Japan. Bull. Hokuriku Natl. Agric. Exp. Stn. 38:113-171 (in Japanese).
  8. Li, X. and De Boeer, S. H. 1995. Selection of polymerase chain reaction primers from an RNA intergenic spacer region for specific detection of Clavibacter michiganensis subsp. sepedonicus. Phytopathology 85:837-842. https://doi.org/10.1094/Phyto-85-837
  9. Noh, T. H., Kang, M. H., Shim, H. K., Paik, C. H., Choi, M. Y. and Kim, H. M. 2012. Simple isolation and identification of Burkholderia glumae from diseased rice seeds. J. Agric. Life Sci. 43:25-27 (in Korean).
  10. Shakya, D. D., Vinther, F. and Mathur, S. B. 1985. Worldwide distribution of a bacterial stripe pathogen of rice identified as Pseudomonas avenae. J. Phytopathol. 114:256-259. https://doi.org/10.1111/j.1439-0434.1985.tb00850.x
  11. Sharma, T. R. 2003. Molecular diagnosis and application of DNA markers in the management of fungal and bacterial plant diseases. Indian J. Biotechnol. 2:99-109.
  12. Song, W. Y., Kim, H. M., Hwang, C. Y. and Schaad, N. W. 2004. Detection of Acidovorax avenae ssp. avenae in rice seeds using BIO-PCR. J. Phytopathol. 152:667-676. https://doi.org/10.1111/j.1439-0434.2004.00914.x
  13. Trung, H. M., Van, N. V., Vien, N. V., Lam, D. T. and Lien, M. 1993. Occurrence of rice grain rot disease in Vietnam. Int. Rice Res. Notes 18:30.