The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Isolation and Characterization of a Bacteriophage Preying an Antifungal Bacterium

  • Rahimi-Midani, Aryan (Department of Microbiology, Pukyong National University) ;
  • Kim, Kyoung-Ho (Department of Microbiology, Pukyong National University) ;
  • Lee, Seon-Woo (Department of Applied Biology, Dong-A University) ;
  • Jung, Sang Bong (Department of Clinical Laboratory Science, Dong-Eui Institute of Technology) ;
  • Choi, Tae-Jin (Department of Microbiology, Pukyong National University)
  • Received : 2016.07.11
  • Accepted : 2016.08.04
  • Published : 2016.12.01
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Abstract

Several Bacillus species were isolated from rice field soils, and 16S rRNA gene sequence analysis showed that Bacillus cereus was the most abundant. A strain named BC1 showed antifungal activity against Rhizoctonia solani. Bacteriophages infecting strain BC1 were isolated from the same soil sample. The isolated phage PK16 had an icosahedral head of $100{\pm}5nm$ and tail of $200{\pm}5nm$, indicating that it belonged to the family Myoviridae. Analysis of the complete linear dsDNA genome revealed a 158,127-bp genome with G + C content of 39.9% comprising 235 open reading frames as well as 19 tRNA genes (including 1 pseudogene). Blastp analysis showed that the proteins encoded by the PK16 genome had the closest hits to proteins of seven different bacteriophages. A neighbor-joining phylogenetic tree based on the major capsid protein showed a robust clustering of phage PK16 with phage JBP901 and BCP8-2 isolated from Korean fermented food.

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References

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