Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Extrusion and Enzyme Treatment on Extraction of β-Glucan from Agaricus blazei Murill

압출성형과 효소처리가 신령버섯 β-Glucan의 추출에 미치는 영향

  • Gil, Sun-Kook (Geonwoo Food & Pharm. Co., Ltd.) ;
  • Shin, Joong-Yup (Geonwoo Food & Pharm. Co., Ltd.) ;
  • Kang, Dae-Il (Department of Food Science and Technology, Kongju National University) ;
  • Ryu, Gi-Hyung (Department of Food Science and Technology, Kongju National University)
  • Received : 2015.07.29
  • Accepted : 2016.02.02
  • Published : 2016.03.31
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Abstract

This study analyzed changes in ${\beta}$-glucan content in Agaricus blazei Murill concentrates according to extrusion and extraction conditions. Screw speed and feed rate were fixed to 250 rpm, and 100 g/min, respectively. Moisture contents (20 and 30%) and barrel temperature (130 and $140^{\circ}C$) were adjusted. ${\beta}$-Glucan content of the extruded sample at a moisture content of 20% and barrel temperature of $130^{\circ}C$ was higher compared to other extrusion conditions. ${\beta}$-Glucan content of the extruded sample at a moisture content of 20% and barrel temperature of $130^{\circ}C$ treated with Rohament CL enzyme was higher compared to Viscozyme L, and Plantase TL enzyme treatments under the same extrusion conditions. In conclusion, extrusion and pretreatment with Rohament CL enzyme enhanced yield of ${\beta}$-glucan extract.

압출성형공정과 효소처리가 신령버섯 추출물의 ${\beta}$-glucan 함량에 미치는 영향을 조사하였다. 스크루 회전속도 250 rpm, 원료 사입량 100 g/min, 사출구 3 mm 원형으로 고정하였으며, 수분 함량 20, 30%, 배럴 온도 130, $140^{\circ}C$로 조절하여 압출성형 신령버섯을 제조하였다. 수분 함량 20%, 배럴 온도 $130^{\circ}C$의 압출성형공정을 통한 신령버섯 추출혼합물은 ${\beta}$-glucan 함량이 16.91 mg/g으로 다른 공정들보다 증가하였다. 수분 함량 20%, 배럴 온도 $130^{\circ}C$의 압출성형 공정과 Rohament CL로 처리한 추출물의 ${\beta}$-glucan 함량은 같은 압출성형공정의 Viscozyme L 처리 추출물 17.35 mg/g과 Plantase TL 처리 추출물 17.51 mg/g보다 18.32 mg/g으로 가장 많이 증가하였다. 결론적으로 압출성형공정과 Rohament CL 처리한 추출물의 ${\beta}$-glucan 함량이 18.32 mg/g으로 압출성형공정과 효소처리를 하지 않은 대조구의 14.45 mg/g보다 약 26.7% 정도 증가함을 나타내었다. 이는 신령버섯 추출 시에 압출성형공정과 효소처리가 ${\beta}$-glucan 함량을 대조구에 비해 증가시킬 수 있다고 판단되었다.

Keywords

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