Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Protective effect of phloroglucinol against gamma radiation-induced oxidative stress in hair follicles

방사선으로 인한 산화적 손상에서 phloroglucinol의 모낭 보호 효과

  • Kim, Areum (Department of Advanced Convergence Technology & Science, Jeju National University) ;
  • Bing, So Jin (Department of Veterinary Medicine, Jeju National University) ;
  • Cho, Jinhee (Department of Veterinary Medicine, Jeju National University) ;
  • Herath, KHINM (Department of Veterinary Medicine, Jeju National University) ;
  • Jeon, You-Jin (Department of Marine Life Sciences, Jeju National University) ;
  • Lee, Byung-Gul (Department of Civil and Enviromental Engineering, Jeju National University) ;
  • Park, Jae-Woo (Department of Nuclear and Energy Engineering, Jeju National University) ;
  • Jee, Youngheun (Department of Advanced Convergence Technology & Science, Jeju National University)
  • 김아름 (제주대학교 차세대융복합과학기술협동과정) ;
  • 빙소진 (제주대학교 수의과대학 수의학과) ;
  • 조진희 (제주대학교 수의과대학 수의학과) ;
  • ;
  • 전유진 (제주대학교 해양과학대학 수산생명의학전공) ;
  • 이병걸 (제주대학교 해양과학대학 토목공학과) ;
  • 박재우 (제주대학교 공과대학 에너지공학과) ;
  • 지영흔 (제주대학교 차세대융복합과학기술협동과정)
  • Received : 2016.01.22
  • Accepted : 2016.03.18
  • Published : 2016.03.31
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Abstract

When exposed to gamma-rays, hair follicular cells immediately go through apoptosis, which hampers their rapid differentiation essential for the regeneration of hair. Phloroglucinol (PG) is a phenolic compound of Ecklonia cava, brown algae abundant in Jeju island, Korea. Containing plentiful polyphenols, PG is known for its instructive effects by inhibiting apoptosis, scavenging oxygen radicals, and protecting cells against oxidative stress. In this study, we demonstrate that PG rescues radiosensitive hair follicular cells from gamma radiation-induced apoptosis and DNA damage. To identify protective capacity of PG on hair follicles, we irradiated with 8.5 Gy (1.5 Gy/min) of gamma-rays to the whole body of C57BL/6 mice at day 6 after depilation with or without PG. In mice exposed to radiation, the expression of proapoptotic molecule p53 was downregulated in the skin of PG treated group. On immunohistochemical observation of the skin, PG inhibited the immunoreactivity of p53 and cleaved caspase-3. PG treatment protected hair follicular cells from cell death due to gamma-radiation. Our results suggest that PG presents radioprotective effects by inhibiting apoptosis of radiosensitive hair follicular cells and can protect hair follicular cells from gamma-ray induced damage.

Keywords

References

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