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Isolation and identification of Vibrio harveyi from chub mackerel (Scomber japonicus)

  • Lee, Young-Ran (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Jun, Jin-Woo (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Giri, Sib Sankar (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Hyoun-Joong (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Yun, Sae-Kil (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Chi, Cheng (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Sang-Guen (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Koh, Jeong-Rack (Lotteworld Aquarium) ;
  • Jung, Ji-Yun (Lotteworld Aquarium) ;
  • Park, Se Chang (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • Received : 2015.12.07
  • Accepted : 2016.02.02
  • Published : 2016.03.31

Abstract

For several days, there was a series of mortalities of chub mackerel (Scomber japonicus) that were reared for public exhibition in a private aquarium in Seoul, Korea. As part of the diagnosis of the dead fish, a bacterial isolate from the kidney was cultured, identified, and confirmed to be Vibrio (V.) harveyi using Vitek System 2 and 16S rRNA gene sequencing. Phylogenetic analysis was also performed by the neighbor-joining method. As a result, the V. harveyi isolated from chub mackerels of a private aquarium in Korea, called as SNUVh-LW1, was clustered in the same group with V. harveyi ATCC33843.

Keywords

References

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