DOI QR코드

DOI QR Code

경기지역 육계의 콕시듐 감염실태 조사

Prevalence of Eimeria infection in commercial broilers in Gyeonggi province, Korea

  • 양병훈 (경기도축산위생연구소 남부지소) ;
  • 심항섭 (경기도축산위생연구소 남부지소)
  • Yang, Byung-Hun (Southern Branch, Gyeonggi Veterinary Service Laboratory) ;
  • Shim, Hang-Sub (Southern Branch, Gyeonggi Veterinary Service Laboratory)
  • 투고 : 2015.09.22
  • 심사 : 2016.03.11
  • 발행 : 2016.03.30

초록

Protozoan parasite, the genus Eimeria, causes an intestinal disease, coccidiosis, in young broilers. Coccidiosis induces significant economic loss in poultry production. This study was designed to identify the prevalence of Eimeria spp. in broilers in Gyeonggi province, Korea. Fecal samples from broilers at 94 farms were collected in two chicken slaughterhouses between March and June 2015. Eimeria infection was determined by microscopic examination and PCR using ITS-1 region. The prevalence of Eimeria was 58.5% (55 of 94). E. acervulina was identified in 96.4% of samples, E. tenella in 81.8%, E. maxima in 12.7%, E. praecox in 12.7%, E. brunetti in 5.5%, E. necatrix in 1.8%, and E. mitis in 1.8%. Body weight (BW) of broilers infected with both E. tenella and E. acervulina ($mean=1.53{\pm}0.19^Bkg$) was significantly lower than broilers with E. acervulina positive/E. tenalla negative ($mean=1.74{\pm}0.07^Akg$) or Eimeria negative ($mean=1.65{\pm}0.15^Akg$) (Duncan's multiple range test, P<0.01). High prevalence of Eimeria tenella and the impact on the broiler body weight shows the importance of this protozoa in broiler industry. Development of the periodic monitoring strategy and systemic management for the purpose of the prevention/eradication of Eimeria infection among broilers is required.

키워드

참고문헌

  1. Ayaz M, Akhtar M, Hayat CS, Hafeez MA, Haq A. 2003. Prevalence of coccidiosis in broiler chickens in Faisalabad, Pakistan Pak Vet J 23: 51-52.
  2. Bachaya HA, Abbas RZ, Raza MA, Iqbal Z, Rehman TU, Baber W and Hussain R. 2015. Existence of coccidiosis and associated risk factors in broiler chickens in Southern Punjab, Pakistan Pak Vet J 35: 81-84.
  3. Bachaya HA, Raza MA, Khan MN, Iqbal Z, Abbas RZ, Murtaza S, Badar N. 2012. Predominance and detection of different Eimeria species causing coccidiosis in layer chickens J Anim Plant Sci 22: 597-600.
  4. Daszak P. 1999. Zoite Migration during Eimeria tenella Infection: Parasite Adaptation to Host Defences Parasitology Today 15: 67-72. https://doi.org/10.1016/S0169-4758(98)01379-9
  5. Del Cacho E, Gallego M, Lopez-Bernad F, Quilez J, Sanchez-Acedo C. 2004. Expression of anti-apoptotic factors in cells parasitized by second-generation schizonts of Eimeria tenella and Eimeria necatrix Vet Parasitol 125: 287-300. https://doi.org/10.1016/j.vetpar.2004.07.017
  6. Dunn A, Keymer A. 1986. Factors affecting the reliability of the McMaster technique J Helminthol 60: 260-262. https://doi.org/10.1017/S0022149X00008464
  7. FitzCoy SH, Edgar SA. 1992. Pathogenicity and control of Eimeria mitis infections in broiler chickens Avain Dis 36: 44-48. https://doi.org/10.2307/1591713
  8. Gordon HM, Whitlock HV. 1939. A new technique for counting nematode eggs in sheep faeces J Coun Sci Ind Res 12: 50-52.
  9. Hamidinejat H, Shapouri MS, Mayahi M, Borujeni MP. 2010. Characterization of Eimeria Species in Commercial Broilers by PCR Based on ITS1 Regions of rDNA Iran J Parasitol 5: 48-54.
  10. Haug A, Gjevre AG, Thebo P, Mattsson JG, Kaldhusdal M. 2008. Coccidial infections in commercial broilers: epidemiological aspects and comparison of Eimeria species identification by morphometric and polymerase chain reaction techniques Avian Pathol 37: 161-170. https://doi.org/10.1080/03079450801915130
  11. Haug A, Thebo P, Mattsson JG. 2007. A simplified protocol for molecular identification of Eimeria species in field samples Vet Parasitol 146: 35-45. https://doi.org/10.1016/j.vetpar.2006.12.015
  12. Haug A, Williams RB, Larsen S. 2006. Counting coccidial oocysts in chicken faeces: a comparative study of a standard McMaster technique and a new rapid method Vet Parasitol 136: 233-242. https://doi.org/10.1016/j.vetpar.2005.11.024
  13. Khan MQ, H Irshad, R Anjum, M Jahangir and U Nasir. 2006. Eimeriosis in poultry of Rawalpindi / Islamabad area Pak Vet J 26: 85-87.
  14. Lee BH, Kim WH, Jeong J, Yoo J, Kwon YK, Jung BY, Kwon JH, Lillehoj HS, Min W. 2010. Prevalence and cross-immunity of Eimeria species on Korean chicken farms J Vet Med Sci. 72: 985-989. https://doi.org/10.1292/jvms.09-0517
  15. Lew AE, Anderson GR, Minchin CM, Jeston PJ, Jorgensen WK. 2003. Inter- and intra-strain variation and PCR detection of the internal transcribed spacer 1 (ITS-1) sequences of Australian isolates of Eimeria species from chickens Vet Parasitol 112: 33-50. https://doi.org/10.1016/S0304-4017(02)00393-X
  16. Mattiello R, Boviez JD, McDougald LR. 2000. Eimeria brunetti and E. necatrix in chickens of Argentina and confirmation of seven species of Eimeria Avian Dis 44: 711-714. https://doi.org/10.2307/1593117
  17. Mouafo AN, Richard F, Entzeroth R. 2000. Observation of sutures in the oocyst wall of Eimeria tenella (Apicomplexa) Parasitol Res 86: 1015-1017. https://doi.org/10.1007/PL00008521
  18. MyungJo Y. 2014. The comparative analysis of infection pattern and oocyst output in Eimeria tenella, E. maxima and E. acervulina in young broiler chicken Veterinary World, EISSN: 2231-0916.
  19. Reid WM, Johnson J. 1970. Coccidia and Coccidiosis, 2nd ed. Akaemine Kiado, Budapest.
  20. Saif YM. Fadly AM. Glisson JR. McDougald LR. Nolan KL. Swayne DE. 2008. Protozoal Infections. pp. 1070-1075. Diseases of Poultry 12th Edition. Blackwell Publishing, USA.
  21. Schnitzler BE, Thebo PL, Mattsson JG, Tomley FM, Shirley MW. 1998. Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic Eimeria species of the chicken Avian Pathol 27: 490-497. https://doi.org/10.1080/03079459808419373
  22. Schnitzler BE, Thebo PL, Tomley FM, Shirley AU & MW. 1999. PCR identication of chicken Eimeria: a simplied read-out Avian Pathol 28: 89-93. https://doi.org/10.1080/03079459995091
  23. Shirley MW, Smith AL, Tomley FM. 2005. The biology of avian Eimeria with an emphasis on their control by vaccination Adv Parasitol 60: 285-330. https://doi.org/10.1016/S0065-308X(05)60005-X
  24. Su YC, Fei AC, Tsai FM. 2003. Differential diagnosis of five avian Eimeria species by polymerase chain reaction using primers derived from the internal transcribed spacer 1 (ITS-1) sequence Vet Parasitol 117: 221-227. https://doi.org/10.1016/j.vetpar.2003.07.028
  25. Thebo P, Lunden A, Uggla A, Hooshmand-RP. 1998. Identification of seven Eimeria species in Swedish domestic fowl Avian Pathology 27: 613-617. https://doi.org/10.1080/03079459808419392
  26. Waldenstedt L, Elwinger K, Lunden A, Thebo P, Uggla A. 2001. Sporulation of Eimeria maxima oocysts in litter with different moisture contents Poult Sci 80: 1412-1415. https://doi.org/10.1093/ps/80.10.1412
  27. Williams RB. 1998. Epidemiological aspects of the use of live anticoccidial vaccines for chickens Int J Parasitol 28: 1089-1098. https://doi.org/10.1016/S0020-7519(98)00066-6