Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Decreasing Particle Size of Paclitaxel Using Polymer in Fractional Precipitation Process

고분자물질을 이용한 분별침전 공정에서 파클리탁셀의 입자크기 감소

  • Kim, Min Jae (Department of Chemical Engineering, Kongju National University) ;
  • Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
  • 김민재 (공주대학교 화학공학부) ;
  • 김진현 (공주대학교 화학공학부)
  • Received : 2015.10.02
  • Accepted : 2015.11.10
  • Published : 2016.04.01
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Abstract

In this study, we have for the first time applied fractional precipitation with hydrophilic polymer in order to decrease the particle size of the anticancer agent paclitaxel from plant cell cultures. When compared with the case where no hydrophilic polymer was employed, the addition of hydrophilic polymer in fractional precipitation resulted in a decrease in the size of the paclitaxel precipitate. Among the polymers used, HPMC 2910 was the most effective for inhibition of precipitate growth. A polymer concentration of 0.2% (w/v) obtained the smallest particle size. The particle size was reduced by ~35% compared to control. In addition, the precipitate size was inversely correlated with the absolute value of the zeta potential.

원료의약품의 활용도를 향상시키기 위하여 입자 크기의 감소는 매우 중요하다. 본 연구에서는 식물세포 유래 항암물질 파클리탁셀의 입자크기 감소를 위하여 친수성 고분자물질을 첨가하여 분별침전을 수행하였다. 고분자물질이 첨가된 분별침전을 통해 입자크기를 감소시킬 수 있었다. 특히 고분자물질 HPMC 2910을 이용한 분별침전의 경우 침전물 성장을 가장 효과적으로 저해함을 알 수 있었다. 고분자물질 HPMC 2910농도 0.2%에서 가장 작은 입자크기의 침전물을 얻을 수 있었는데, 대조군 대비 ~35% 정도로 입자크기를 감소시킬 수 있었다. 또한 파클리탁셀 침전물의 입자크기는 친수성 고분자물질 첨가에 따른 침전용액의 제타전위 절대값에 반비례함을 알 수 있었다.

Keywords

References

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