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Anti-allergic effect of Lactobacillus rhamnosus IDCC 3201 isolated from breast milk-fed Korean infant

한국인 모유영양아의 분변에서 분리한 Lactobacillus rhamnosus IDCC 3201의 항 알레르기 효과

  • Lee, Seung-Hun (Bioprocess Engineering Team, Research Laboratories, ILDONG Pharmaceutical Co., Ltd.) ;
  • Kang, Jae-Hoon (Bioprocess Engineering Team, Research Laboratories, ILDONG Pharmaceutical Co., Ltd.) ;
  • Kang, Dae-Jung (Bioprocess Engineering Team, Research Laboratories, ILDONG Pharmaceutical Co., Ltd.)
  • Received : 2016.03.03
  • Accepted : 2016.03.16
  • Published : 2016.03.31

Abstract

We investigated 23 lactic acid bacteria isolated from Korean breast milk-fed infant in order to select strains which show superior anti-allergic effect. The candidates were cultivated and then we obtained dried powders of tyndallized cells and supernatant concentrate separately. Screening was carried out with down-regulation of interleukin (IL) 4 and up-regulation of IFN-${\gamma}$ in mouse splenocytes. As a result of the screening, we selected Lactobacillus rhamnosus IDCC 3201 (RH3201) for oral feeding to ovalbumin-sensitized BALB/c mice. Oral administration of RH3201 as dead cell bodies and supernatant concentrate suppressed hyper-production of serum immunoglobulin (Ig) E levels compared to vehicle group. Such anti-allergic effects were achieved by improvement of the balance between cytokines produced from type-1 helper T (Th1) and type-2 helper T (Th2) lymphocytes. Therefore, RH3201 has potential to improve atopic symptoms by immunomodulatory effect.

본 연구에서는 우수한 아토피 완화능을 가진 유산균을 선별하기 위해 한국인 유아 분변으로부터 23종의 유산균을 분리하였다. 후보 균주들을 배양하여 열처리 된 세포와 상등액 농축물을 각각 얻었다. 우수 균주 선별은 마우스 비장세포를 이용하여 IL-4의 억제 및 IFN-${\gamma}$의 증가 정도를 확인하는 실험을 통해 진행되었다. 선별 실험 결과로 Lactobacillus rhamnosus IDCC 3201 (RH3201)을 OVA로 면역 반응을 유발시킨 BALB/c 마우스에 투여할 유산균으로 선정하였다. RH3201의 균체와 대사물을 경구 투여한 군에서는 유발군에 비해 혈중 IgE의 과다 생성이 억제된 것을 확인하였다. 그러한 알레르기 억제능은 type-1 T helper (Th1) 세포와 type-2 T helper (Th2) 세포의 싸이토카인 간의 균형을 향상시킴으로써 유도되었다. 따라서 RH3201의 균체와 배양물은 면역 조절을 통해 아토피 증상을 완화시킬 수 있음을 확인하였다.

Keywords

References

  1. Borish, L. 1998. Updates on cell and cytokine. J. Allergy Clin. Immun. 101, 293-297. https://doi.org/10.1016/S0091-6749(98)70238-6
  2. Chauviere, G., Coconnier, M.H., Kerneis, S., Arlette, D.M., Joly, B., and Alain, L.S. 1992. Competitive exclusion of diarrheagenic Escherichia coli (ETEC) from human enterocyte-like Caco-2 cells by heat-killed Lactobacillus. FEMS Microbiol. Lett. 91, 213-217. https://doi.org/10.1111/j.1574-6968.1992.tb05211.x
  3. Claes, I., Segers, M., Verhoeven, T., Dusselier, M., Sels, B., Keersmaecker, S., Vanderleyden, J., and Lebeer, S. 2012. Lipoteichoic acid is an important microbe-associated molecular pattern of Lactobacillus rhamnosus GG. Microb. Cell Fact. 11, 161.
  4. Cross, M.L., Stevenson, L.M., and Gill, H.S. 2001. Anti-allergy properties of fermented foods : an important immunoregulatory mechanism of lactic acid bacteria. Int. Immunopharmacol. 1, 891-901. https://doi.org/10.1016/S1567-5769(01)00025-X
  5. Fujiwara, D., Inoue, S., Wakabayashi, H., and Fujii, T. 2004. The anti-allergic effects of lactic acid bacteria are strain dependent and mediated by effects on both Th1/Th2 cytokine expression and balance. Int. Arch. Allergy Immunol. 135, 205-215. https://doi.org/10.1159/000081305
  6. Gail, C.M. 2007. Use of probiotics: Benefits of a balanced microbiome in the intestinal tract. National Parent Club Canine Health Conference pp. 19-21.
  7. Herich, R. and Levkut, M. 2002. Lactic acid bacteria, probiotics and immune system. Vet. Med. Czech. 47, 169-180.
  8. Ishida, Y., Nakamura, F., Kanzato, H., Sawada, D., Hirata, H., Nishimura, A., Kajimoto, O., and Fujiwara, S. 2005. Clinical effects of Lactobacillus acidophilus strain L-92 on perennial allergic rhinitis : A double-blind, placebo-controlled study. J. Dairy Sci. 88, 527-533. https://doi.org/10.3168/jds.S0022-0302(05)72714-4
  9. Kato, I., Tanaka, K., and Yokokura, T. 1999. Lactic acid bacterium potently induces the production of interleukin-12 and interferon-$\gamma$ by mouse splenocytes. Int. J. Immunopharmacol. 21, 121-131. https://doi.org/10.1016/S0192-0561(98)00072-1
  10. Kelly, D., Conway, S., Aminov, R. 2005. Commensal gut bacteria: mechanisms of immune modulation. Trends Immunol. 26, 326-333. https://doi.org/10.1016/j.it.2005.04.008
  11. Majamaa, H. and Isolauri, E. 1997. Probiotics : A novel approach in the management of food allergy. J. Allergy Clin. Immunol. 99, 179-185. https://doi.org/10.1016/S0091-6749(97)70093-9
  12. Retnaninggtyas, L.P., Sudarmo, S.M., Harsono, A., and Damanik, S.M. 2008. Effect of probiotic on the fecal sIgA level in preterm infants. Paediatr. Indones 48, 246-252.
  13. Schwandner, R., Dziarski, R., Wesche, H., Rothe, M., and Kirsehning, C.J. 1999. Peptidoglycan and lipoteichoic acid-induced cell activation is mediated by toll-like receptor 2. J. Biol. Chem. 274, 1746-1749.
  14. Styriak, I., Nemcova, R., Chang, Y.H., and Ljungh, A. 2003. Binding of extracellular matrix molecules by probiotic bacteria. Lett. Appl. Microbiol. 37, 329-333. https://doi.org/10.1046/j.1472-765X.2003.01402.x
  15. Vercelli, D. 1995. Immunoglobulin E regulation in humans, 1989-1994. Allergy, 50, 5-8.
  16. Xu, M., Zhao, M., Yang, R., Zhang, Z., Li, Y., and Wang, J. 2013. Effect of dietary nucleotides on immune function in Balb/C mice. Int. Immunopharmacol. 17, 50-56. https://doi.org/10.1016/j.intimp.2013.04.032
  17. Yazdanbakhsh, M., Kremsner, P.G., and van Ree, R. 2002. Allergy, parasites, and the hygiene hypothesis. Science 296, 490-494. https://doi.org/10.1126/science.296.5567.490
  18. Yu, J.H., Jang, S.O., Kim, B.J., Song, Y.H., Kwon, J.W., Kang, M.J., Choi, W.A., Jung, H.D., and Hong, S.J. 2010. The effects of Lactobacillus rhamnosus on the prevention of asthma in a murine model. Allergy Asthma Immunol. Res. 2, 199-205. https://doi.org/10.4168/aair.2010.2.3.199

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