Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Green Tea (-)-Epigallotocatechin-3-Gallate Induces PGC-1α Gene Expression in HepG2 Cells and 3T3-L1 Adipocytes

  • Lee, Mak-Soon (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Lee, Seohyun (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Doo, Miae (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Kim, Yangha (Department of Nutritional Science and Food Management, Ewha Womans University)
  • Received : 2015.10.07
  • Accepted : 2016.01.05
  • Published : 2016.03.31
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Abstract

Green tea (Camellia sinensis) is one of the most popular beverages in the world and has been acknowledged for centuries as having significant health benefits. (-)-Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea, and it has been reported to have health benefit effects. Peroxisome proliferator-activated receptor ${\gamma}$ coactivator $(PGC)-1{\alpha}$ is a crucial regulator of mitochondrial biogenesis and hepatic gluconeogenesis. The objective of this study was to investigate whether EGCG from green tea can affect the ability of transcriptional regulation on $PGC-1{\alpha}$ mRNA expression in HepG2 cells and 3T3-L1 adipocytes. To study the molecular mechanism that allows EGCG to control $PGC-1{\alpha}$ expression, the promoter activity levels of $PGC-1{\alpha}$ were examined. The $PGC-1{\alpha}$ mRNA level was measured using quantitative real-time PCR. The -970/+412 bp of $PGC-1{\alpha}$ promoter was subcloned into the pGL3-Basic vector that includes luciferase as a reporter gene. EGCG was found to up-regulate the $PGC-1{\alpha}$ mRNA levels significantly with $10{\mu}mol/L$ of EGCG in HepG2 cells and differentiated 3T3-L1 adipocytes. $PGC-1{\alpha}$ promoter activity was also increased by treatment with $10{\mu}mol/L$ of EGCG in both cells. These results suggest that EGCG may induce $PGC-1{\alpha}$ gene expression, potentially through promoter activation.

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References

  1. Yang CS, Lambert JD, Ju J, Lu G, Sang S. 2007. Tea and cancer prevention: molecular mechanisms and human relevance. Toxicol Appl Pharmacol 224: 265-273. https://doi.org/10.1016/j.taap.2006.11.024
  2. Wolfram S, Wang Y, Thielecke F. 2006. Anti-obesity effects of green tea: from bedside to bench. Mol Nutr Food Res 50: 176-187. https://doi.org/10.1002/mnfr.200500102
  3. Grove KA, Lambert JD. 2010. Laboratory, epidemiological, and human intervention studies show that tea (Camellia sinensis) may be useful in the prevention of obesity. J Nutr 140: 446-453. https://doi.org/10.3945/jn.109.115972
  4. Lee MS, Kim CT, Kim Y. 2009. Green tea (-)-epigallocatechin-3-gallate reduces body weight with regulation of multiple genes expression in adipose tissue of diet-induced obese mice. Ann Nutr Metab 54: 151-157. https://doi.org/10.1159/000214834
  5. Lin J, Handschin C, Spiegelman BM. 2005. Metabolic control through the PGC-1 family of transcription coactivators. Cell Metab 1: 361-370. https://doi.org/10.1016/j.cmet.2005.05.004
  6. Puigserver P. 2005. Tissue-specific regulation of metabolic pathways through the transcriptional coactivator PGC1-$\alpha$. Int J Obes 29: S5-S9. https://doi.org/10.1038/sj.ijo.0802905
  7. Puigserver P, Wu Z, Park CW, Graves R, Wright M, Spiegelman BM. 1998. A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Cell 92: 829-839. https://doi.org/10.1016/S0092-8674(00)81410-5
  8. Canto C, Auwerx J. 2009. PGC-$1{\alpha}$, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Curr Opin Lipidol 20: 98-105. https://doi.org/10.1097/MOL.0b013e328328d0a4
  9. Wright DC, Han DH, Garcia-Roves PM, Geiger PC, Jones TE, Holloszy JO. 2007. Exercise-induced mitochondrial biogenesis begins before the increase in muscle PGC-$1{\alpha}$ expression. J Biol Chem 282: 194-199. https://doi.org/10.1074/jbc.M606116200
  10. Klaus S, Pultz S, Thone-Reineke C, Wolfram S. 2005. Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int J Obes 29: 615-623. https://doi.org/10.1038/sj.ijo.0802926
  11. Lee MS, Kim CT, Kim IH, Kim Y. 2009. Inhibitory effects of green tea catechin on the lipid accumulation in 3T3-L1 adipocytes. Phytother Res 23: 1088-1091. https://doi.org/10.1002/ptr.2737
  12. Lee MS, Kim Y. 2009. (-)-Epigallocatechin-3-gallate enhances uncoupling protein 2 gene expression in 3T3-L1 adipocytes. Biosci Biotechnol Biochem 73: 434-436. https://doi.org/10.1271/bbb.80563
  13. Lee MS, Park JY, Freake H, Kwun IS, Kim Y. 2008. Green tea catechin enhances cholesterol $7{\alpha}$-hydroxylase gene expression in HepG2 cells. Br J Nutr 99: 1182-1185.
  14. Lee MS, Shin Y, Jung S, Kim CT, Kim IH, Kim Y. 2015. Effects of high hydrostatic pressure extract of Korean fresh ginseng on hepatic lipid accumulation and AMPK activation in HepG2 cells. J Food Nutr Res 3: 40-45. https://doi.org/10.12691/jfnr-3-1-7
  15. Rozen S, Skaletsky H. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mole Biol 132: 365-386.
  16. Fujiki H, Imai K, Nakachi K, Shimizu M, Moriwaki H, Suganuma M. 2012. Challenging the effectiveness of green tea in primary and tertiary cancer prevention. J Cancer Res Clin Oncol 138: 1259-1270. https://doi.org/10.1007/s00432-012-1250-y
  17. Suganuma M, Okabe S, Oniyama M, Tada Y, Ito H, Fujiki H. 1998. Wide distribution of [$^3H$](-)-epigallocatechin gallate, a cancer preventive tea polyphenol, in mouse tissue. Carcinogenesis 19: 1771-1776. https://doi.org/10.1093/carcin/19.10.1771
  18. Chan CY, Wei L, Castro-Munozledo F, Koo WL. 2011. (-)-Epigallocatechin-3-gallate blocks 3T3-L1 adipose conversion by inhibition of cell proliferation and suppression of adipose phenotype expression. Life Sci 89: 779-785. https://doi.org/10.1016/j.lfs.2011.09.006
  19. Kim JJY, Tan Y, Xiao L, Sun YL, Qu X. 2013. Green tea polyphenol epigallocatechin-3-gallate enhance glycogen synthesis and inhibit lipogenesis in hepatocytes. BioMed Res Int 2013: 920128.
  20. Yoon JC, Puigserver P, Chen G, Donovan J, Wu Z, Rhee J, Adelmant G, Stafford J, Kahn CR, Granner DK, Newgard CB, Spiegelman BM. 2001. Control of hepatic gluconeogenesis through the transcriptional coactivator PGC-1. Nature 413: 131-138. https://doi.org/10.1038/35093050
  21. Matravadia S, Martino VB, Sinclair D, Mutch DM, Holloway GP. 2013. Exercise training increases the expression and nuclear localization of mRNA destabilizing proteins in skeletal muscle. Am J Physiol Regul Integr Comp Physiol 305: R822-R831. https://doi.org/10.1152/ajpregu.00590.2012
  22. Jun HJ, Joshi Y, Patil Y, Noland RC, Chang JS. 2014. NTPGC-$1{\alpha}$ activation attenuates high-fat diet-induced obesity by enhancing brown fat thermogenesis and adipose tissue oxidative metabolism. Diabetes 63: 3615-3625. https://doi.org/10.2337/db13-1837
  23. Valenti D, De Rasmo D, Signorile A, Rossi L, de Bari L, Scala I, Granese B, Papa S, Vacca RA. 2013. Epigallocatechin-3-gallate prevents oxidative phosphorylation deficit and promotes mitochondrial biogenesis in human cells from subjects with Down's syndrome. Biochim Biophys Acta 1832: 542-552. https://doi.org/10.1016/j.bbadis.2012.12.011
  24. Ye Q, Ye L, Xu X, Huang B, Zhang X, Zhu Y, Chen X. 2012. Epigallocatechin-3-gallate suppresses 1-methyl-4-phenylpyridine-induced oxidative stress in PC12 cells via the SIRT1/PGC-$1{\alpha}$ signaling pathway. BMC Complement Altern Med 12: 82. https://doi.org/10.1186/1472-6882-12-82
  25. Mukherjee S, Siddiqui MA, Dayal S, Ayoub YZ, Malathi K. 2014. Epigallocatechin-3-gallate suppresses proinflammatory cytokines and chemokines induced by Toll-like receptor 9 agonists in prostate cancer cells. J Inflamm Res 7: 89-101.

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