Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng

  • Sun, Hang (Jilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University) ;
  • Liu, Fangbing (College of Traditional Chinese Medicine, China Pharmaceutical University) ;
  • Sun, Liwei (Jilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University) ;
  • Liu, Jianzeng (Jilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University) ;
  • Wang, Manying (Jilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University) ;
  • Chen, Xuenan (Research Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine) ;
  • Xu, Xiaohao (Jilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University) ;
  • Ma, Rui (Jilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University) ;
  • Feng, Kai (Jilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University) ;
  • Jiang, Rui (Jilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University)
  • Received : 2015.01.08
  • Accepted : 2015.06.01
  • Published : 2016.04.15
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Abstract

Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.

Keywords

References

  1. Choi YE. Ginseng (Panax ginseng). Methods Mol Biol 2006;344:361-71.
  2. Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pharmacol 1999;58:1685-93. https://doi.org/10.1016/S0006-2952(99)00212-9
  3. Wang J, Li SS, Fan YY, Chen Y, Liu D, Cheng HR, Gao XG, Zhou YF. Anti-fatigue activity of the water-soluble polysaccharides isolated from Panax ginseng C. A. Meyer. J Ethnopharmacol 2010;130:421-3. https://doi.org/10.1016/j.jep.2010.05.027
  4. Wang TS. Chinese ginseng. Shenyang (LN): Liaoning Scientific and Technical Publishers; 2001.
  5. Qi LW, Wang CZ, Yuan CS. Isolation and analysis of ginseng: advances and challenges. Nat Prod Rep 2011;28:467-95. https://doi.org/10.1039/c0np00057d
  6. Qi LW, Wang CZ, Yuan CS. Ginsenosides from American ginseng: chemical and pharmacological diversity. Phytochemistry 2011;72:689-99. https://doi.org/10.1016/j.phytochem.2011.02.012
  7. Qi LW, Wang CZ, Yuan CS. American ginseng: potential structureefunction relationship in cancer chemoprevention. Biochem Pharmacol 2010;80:947-54. https://doi.org/10.1016/j.bcp.2010.06.023
  8. Christensen LP. Ginsenosides chemistry, biosynthesis, analysis and potential health effects. Adv Food Nutr Re 2009;55:1-99.
  9. Yun TK. Panax ginseng a non-organ-specific cancer preventive? Lancet Oncol 2001;2:49-55. https://doi.org/10.1016/S1470-2045(00)00196-0
  10. Park CK, Jeon BS, Yang JW. The chemical components of Korean ginseng. Food Ind Nutr 2003;8:10-23.
  11. Zhang YX, Liu SM, Dai SY, Yuan JS. Integration of shot-gun proteomics and bioinformatics analysis to explore plant hormone responses. BMC Bioinformatics 2012;13:S8.
  12. Ross PL, Huang YN, Marchese JN, Williamson B, Parker K, Hattan S, Khainovski N, Pillai S, Dey S, Daniels S, et al. Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol Cell Proteomics 2004;3:1154-69. https://doi.org/10.1074/mcp.M400129-MCP200
  13. He Y, Dai SJ, Dufresne CP, Zhu N, Pang QY, Chen SX. Integrated proteomics and metabolomics of Arabidopsis acclimation to gene-dosage dependent perturbation of isopropylmalate dehydrogenases. PLoS One 2013;8:e57118. https://doi.org/10.1371/journal.pone.0057118
  14. Shingaki-Wells RN, Huang S, Taylor NL, Carroll AJ, Zhou W, Millar AH. Differential molecular responses of rice and wheat coleoptiles to anoxia reveal novel metabolic adaptations in amino acid metabolism for tissue tolerance. Plant Physiol 2011;156:1706-24. https://doi.org/10.1104/pp.111.175570
  15. Baum G, Lev-Yadun S, Fridmann Y, Arazi T, Katsnelson H, Zik M, Fromm H. Calmodulin binding to glutamate decarboxylase is required for regulation of glutamate and GABA metabolism and normal development in plants. EMBO J 1996;15:2988-96.
  16. Kant S, Bi YM, Weretilnyk E, Barak S, Rothstein SJ. The Arabidopsis halophytic relative Thellungiella halophila tolerates nitrogen-limiting conditions by maintaining growth, nitrogen uptake, and assimilation. Plat Physiol 2008;147:1168-80. https://doi.org/10.1104/pp.108.118125
  17. Zhang SJ, Li N, Gao F, Yang AF, Zhang JR. Over-expression of TsCBF1 gene confers improved drought tolerance in transgenic maize. Mol Breeding 2010;26:455-65. https://doi.org/10.1007/s11032-009-9385-5
  18. Lin MW, Watson JF, Baggett JR. Inheritance of soluble solids and pyruvic acid content of bulb onions. J Am Soc Hort Sci 1995;120:119-22.
  19. Kortt AA, Liu TY. Mechanism of action of streptococcal proteinase. I. Activesite titration. Biochemistry 1973;12:320-7. https://doi.org/10.1021/bi00726a023
  20. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7. https://doi.org/10.1016/0003-9861(59)90090-6
  21. Husted S, Schjoerring JK. Apoplastic pH and ammonium concentration in leaves of Brassica napus L. Plant Physiol 1995;109:1453-60. https://doi.org/10.1104/pp.109.4.1453
  22. Cooper TG, Beevers H. Mitochondria and glyoxysomes from castor bean endosperm. J Biol Chem 1969;244:3507-13.
  23. Wang XQ, Yang PF, Liu Z, Liu WZ, Hu Y, Chen H, Kuang TY, Pei ZM, Shen SH, He YK. Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy. Plant Physiol 2009;149:1739-50. https://doi.org/10.1104/pp.108.131714
  24. Chinnasamy G, Rampitsch C. Efficient solubilization buffers for twodimensional gel electrophoresis of acidic and basic proteins extracted from wheat seeds. Biochim Biophys Acta 2006;1764:641-4. https://doi.org/10.1016/j.bbapap.2005.10.003
  25. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;7:248-54.
  26. Sun LW, Ma PT, Li DN, Lei XJ, Ma R, Qi C. Protein extraction from the stem of Panax ginseng C. A. Meyer: a tissue of lower protein extraction efficiency for proteomic analysis. Afr J Biotechnol 2011;10:4328-33.
  27. Mathesius U, Djordjevic MA, Oakes M, Goffard N, Haerizadeh F, Weiller GF, Singh MB, Bhalla PL. Comparative proteomic profiles of the soybean (Glycine max) root apex and differentiated root zone. Proteomics 2010;11:1707-19.
  28. Plaxton WC. The organization and regulation of plant glycolysis. Annu Rev Plant Physiol Plant Mol Biol 1996;47:185-214. https://doi.org/10.1146/annurev.arplant.47.1.185
  29. Cramer GR, Sluyter SC, Hopper DW, Pascovici D, Keighley T, Haynes PA. Proteomic analysis indicates massive changes in metabolism prior to the inhibition of growth and photosynthesis of grapevine (Vitis vinifera L.) in response to water deficit. BMC Plant Biology 2013;13:49. https://doi.org/10.1186/1471-2229-13-49
  30. Fernie AR, Carrari F, Sweetlove LJ. Respiratory metabolism: glycolysis, the TCA cycle and mitochondrial electron transport. Curr Opin Plant Biol 2004;7:254-61. https://doi.org/10.1016/j.pbi.2004.03.007
  31. Millar AH, Whelan J, Soole KL, Day DA. Organization and regulation of mitochondrial respiration in plants. Annu Rev Plant Biol 2011;62:79-104. https://doi.org/10.1146/annurev-arplant-042110-103857
  32. Meyer AJ, Hell R. Glutathione homeostasis and redox regulation by sulfhydryl groups. Photosynth Res 2005;86:435-57. https://doi.org/10.1007/s11120-005-8425-1
  33. Colville L, Kranner I. Desiccation tolerant plants as model systems to study redox regulation of protein thiols. Plant Growth Regul 2010;62:241-55. https://doi.org/10.1007/s10725-010-9482-9
  34. Harms K, von Ballmoos P, Brunold C, Hofgen R, Hesse H. Expression of a bacterial serine acetyltransferase in transgenic potato plants leads to increased levels of cysteine and glutathione. Plant J 2000;22:335-43. https://doi.org/10.1046/j.1365-313x.2000.00743.x
  35. Ruiz J, Blumwald E. Salinity-induced glutathione synthesis in Brassica napus. Planta 2002;214:965-9. https://doi.org/10.1007/s00425-002-0748-y
  36. Good AG, Zaplachinski ST. The effects of drought stress on free amino acid accumulation and protein synthesis in Brassica napus. Physiol Plantarum 1994;90:9-14. https://doi.org/10.1111/j.1399-3054.1994.tb02185.x
  37. Gzik A. Accumulation of proline and pattern of alpha-amino acids in sugar beet plants in response to osmotic, water and salt stress. Environ Exp Bot 1996;36:29-38. https://doi.org/10.1016/0098-8472(95)00046-1
  38. Noctor G, Mhamdi A, Chaouch S, Han Y, Neukermans J, Marquez-Garcia B, Queval G, Foyer CH. Glutathione in plants: an integrated overview. Plant Cell Environ 2012;35:454-84. https://doi.org/10.1111/j.1365-3040.2011.02400.x
  39. Bashir H, Ahmad J, Bagheri R, Nauman M, Qureshi MI. Limited sulfur resource forces Arabidopsis thalianato shift towards non-sulfur tolerance under cadmium stress. Environ Exp Bot 2013;94:19-32. https://doi.org/10.1016/j.envexpbot.2012.05.004
  40. Iyer SS, Ramirez AM, Ritzenthaler JD, Torres-Gonzalez E, Roser-Page S, Mora AL, Brigham KL, Jones DP, Roman J, Rojas M. Oxidation of extracellular cysteine/cystine redox state in bleomycin-induced lung fibrosis. Am J Physiol Lung Cell Mol Physiol 2009;296:37-45. https://doi.org/10.1152/ajplung.90401.2008
  41. Krauth-Siegel RL, Leroux AE. Low-molecular-mass antioxidants in parasites. Antioxid Redox Sign 2012;17:583-607. https://doi.org/10.1089/ars.2011.4392
  42. Zagorchev L, Seal CE, Kranner I, Odjakova M. Redox state of low-molecularweight thiols and disulphides during somatic embryogenesis of salt-treated suspension cultures of Dactylis glomerata L. Free Radic Res 2012;46:656-64. https://doi.org/10.3109/10715762.2012.667565
  43. Dos Santos CV, Cuine S, Rouhier N, Rey P. The Arabidopsis plastidic methionine sulfoxide reductase B proteins. Sequence and activity characteristics, comparison of the expression with plastidic methionine sulfoxide reductase A, and induction by photooxidative stress. Plant Physiol 2005;138:909-22. https://doi.org/10.1104/pp.105.062430
  44. Alcázar R, Altabella T, Marco F, Bortolotti C, Reymond M, Koncz C, Carrasco P, Tiburcio AF. Polyamines: molecules with regulatory functions in plant abiotic stress tolerance. Planta 2010;231:1237-49. https://doi.org/10.1007/s00425-010-1130-0
  45. Van de Poel B, Bulens I, Oppermann Y, Hertog ML, Nicolai BM, Sauter M, Geeraerd AH. S-adenosyl-l-methionine usage during climacteric ripening of tomato in relation to ethylene and polyamine biosynthesis and transmethylation capacity. Physiol Plantarum 2013;148:176-88. https://doi.org/10.1111/j.1399-3054.2012.01703.x
  46. Normanly J, Cohen JD, Fink GR. Arabidopsis thaliana auxotrophs reveal a tryptophan-independent biosynthetic pathway for indole-3-acetic acid. Proc Natl Acad Sci USA 1993;90:10355-9. https://doi.org/10.1073/pnas.90.21.10355
  47. Leyser O. Auxin signalling: the beginning, the middle and the end. Curr Opin Plant Biol 2001;4:382-6. https://doi.org/10.1016/S1369-5266(00)00189-8
  48. Gut H, Dominici P, Pilati S, Astegno A, Petoukhov MV, Svergun DI, Grütter MG, Capitani G. A common structural basis for pH- and calmodulin-mediated regulation in plant glutamate decarboxylase. J Mol Biol 2009;392:334-51. https://doi.org/10.1016/j.jmb.2009.06.080
  49. Tower DB, Roberts E. Inhibition the nervous system and GABA. California: Pergamon Press; 1960.

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