Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Prevalence and Factors Associated with Opisthorchis viverrini Infection in Khammouane Province, Lao PDR

  • Saiyachak, Khamphanavanh (Epidemiology and Biostatistics Section, Faculty of Public Health, Khon Kaen University) ;
  • Tongsotsang, Sutthiporn (Epidemiology and Biostatistics Section, Faculty of Public Health, Khon Kaen University) ;
  • Saenrueang, Thitima (Epidemiology and Biostatistics Section, Faculty of Public Health, Khon Kaen University) ;
  • Moore, Malcolm A (Epidemiology and Biostatistics Section, Faculty of Public Health, Khon Kaen University) ;
  • Promthet, Supannee (Epidemiology and Biostatistics Section, Faculty of Public Health, Khon Kaen University)
  • Published : 2016.04.11
Download PDF
⟨ Previous Next ⟩

Abstract

Opisthorchis viverrini (OV) liver flukes are common parasites found in central and southern Laos and constitute a major public health problem in the country. Laos people continue to have the habit of extensively consuming raw or half-cooked fish which are intermediate hosts. This study aimed to determine the prevalence and factors associated with OV infection in the population of Thakek district, Khammouane Province. This cross-sectional analytic study covered 237 subjects who filled out structured questionnaires. Fecal examination for OV infection was performed by Kato's thick smear method. Data analysis was carried out using STATA Version 10.0. Multiple logistic regression was applied. The results showed that the infection rate of OV was 54.8%. Factors associated with OV infections were gender, a habit of defecation in fields and raw fish (goi bplaa dip) consumption. Opisthorchiasis and associated cholangiocarcinoma development thus appear to remain as important concerns in Laos.

Keywords

References

  1. Arimatsu Y, Kaewkes S, Laha T, Hong SJ, Sripa B (2012). Rapid detection of Opisthorchis viverrini copro-DNA using loop-mediated isothermal amplification (LAMP). Parasitol Int, 61, 178-82 https://doi.org/10.1016/j.parint.2011.08.009
  2. Arimatsu Y, Kaewkes S, Laha T, Sripa B (2015). Specific diagnosis of Opisthorchis viverrini using loop-mediated isothermal amplification (LAMP) targeting parasite microsatellites. Acta Trop, 141, 368-71. https://doi.org/10.1016/j.actatropica.2014.09.012
  3. Aunpromma S, Tangkawattana P, Papirom P, et al (2012). High prevalence of Opisthorchis viverrini infection in reservoir hosts in four districts of Khon Kaen Province, an opisthorchiasis endemic area of Thailand. Parasitol Int, 61, 60-4. https://doi.org/10.1016/j.parint.2011.08.004
  4. Bedier E, Chesneau P (1929). Distomatose hepatique a Opisthorchis in Laos (Vientiane and Thakek). Bull Soc Path Exot, 22, 331-4.
  5. Bogoch II, Sayasone S, Vonghachack Y, et al (2016). Diagnosis of Opisthorchis viverrini infection with handheld microscopy in Lao People's Democratic Republic. Am J Trop Med Hyg, 94, 158-60. https://doi.org/10.4269/ajtmh.15-0525
  6. Buathong S, Leelayoova S, Mungthin M, et al (2015). Development and evaluation of PCR methods based on cytochrome c oxidase subunit one (COX1) and NADH dehydrogenase subunit one gene (nad1) to detect Opisthorchis viverrini in human fecal samples. Parasitol Res, 114, 3547-9. https://doi.org/10.1007/s00436-015-4640-7
  7. Chaiputcha K, Promthet S, Bradshaw P (2015). Prevalence and risk factors for infection by Opisthorchis viverrini in an urban area of Mahasarakham province, northeast Thailand. Asian Pac J Cancer Prev, 16, 4173-6. https://doi.org/10.7314/APJCP.2015.16.10.4173
  8. Chudthaisong N, Promthet S, Bradshaw P (2015). Risk factors for Opisthorchis viverrini infection in Nong Khai province, Thailand. Asian Pac J Cancer Prev, 16, 4593-6. https://doi.org/10.7314/APJCP.2015.16.11.4593
  9. Doanh PN, Nawa Y (2016). Clonorchis sinensis and Opisthorchis spp. in Vietnam: current status and prospects. Trans R Soc Trop Med Hyg, 110, 13-20. https://doi.org/10.1093/trstmh/trv103
  10. Enes JE, Wages AJ, Malone JB, Tesana S (2010). Prevalence of Opisthorchis viverrini infection in the canine and feline hosts in three villages, Khon Kaen Province, northeastern Thailand. Southeast Asian J Trop Med Publ Health, 41, 36-42.
  11. Forrer A, Sayasone S, Vounatsu P, et al (2012). Spatial distribution of, and risk factors for, Opisthorchis viverrini infection in Southern Lao PDR. PLoS Negl Trop Dis, 6, 1481. https://doi.org/10.1371/journal.pntd.0001481
  12. Haswell-Elkins MR, Mairiang P, Chaiyakhum J, et al (1994). Cross-sectional study of Opisthorchis viverrini infection and cholangiocarcinoma in communities within a high-risk area in northeast Thailand. Int J Cancer, 59, 505-9. https://doi.org/10.1002/ijc.2910590412
  13. Hsieh FY, Bloch DA, Larsen MD (1998). A simple method of sample size calculation for linear and logistic regresstion. Statistics Med, 17, 1623-34. https://doi.org/10.1002/(SICI)1097-0258(19980730)17:14<1623::AID-SIM871>3.0.CO;2-S
  14. IARC (1994). Infection with liver flukes (Opisthorchis viverrini, Opisthorchis felineus and Clonorchis sinensis). IARC Monogr Eval Carcinog Risks Hum, 61, 121-75.
  15. Jamornthayawat N (2002). The diagnosis of human opisthorchiasis. Southeast Asian J Tropical Med Public Health; 33, 86-91.
  16. Jongsuksuntigul P, Imsomboon T (1997). The impact of a decade long opisthorchis control program in northeastern, Thailand. Southeast Asian J Trop Med Public Health, 28, 551-7.
  17. Kaewpitoon N, Kootanavanichpong N, Kompor P, et al (2015). Review and current status of Opisthorchis viverrini infection at the community level in Thailand. Asian Pac J Cancer Prev, 16, 7375-9. https://doi.org/10.7314/APJCP.2015.16.16.7375
  18. Kaewpitoon SJ, Rujirakul R, Tongtawee T, et al (2016). Detection of the carcinogenic liver fluke Opisthorchis viverrini using a mini parasep SF faecal parasite concentrator. Asian Pac J Cancer Prev, 17, 373-376. https://doi.org/10.7314/APJCP.2016.17.1.373
  19. Kamsa-Ard S, Luvira V, Pugkhem A3, et al (2015). Association between praziquantel treatment and cholangiocarcinoma: a hospital-based matched case-control study. BMC Cancer, 15, 776. https://doi.org/10.1186/s12885-015-1788-6
  20. Khemphavanh M, Chalit K, Jitra W, Prayong R (2009). Opisthorchis viverrini metacercariae in cyprinoid fish from three river in Khammouane province, Lao PDR. J Trop Med Parasitol, 32, 23-9.
  21. Khoontawad J, Laothong U, Roytrakul S, et al (2012). Proteomic identification of plasma protein tyrosine phosphatase alpha and fibronectin associated with liver fluke, Opisthorchis viverrini, infection. PLoS One, 7:e45460. https://doi.org/10.1371/journal.pone.0045460
  22. Kobayashi J, Vannachone B, Sato Y, et al (2000). An epidemiological study on Opisthorchis viverrini infection in Lao villages. Southeast Asian J Trop Med Public Health, 31, 128-32.
  23. Le TH, Nguyen NT, Truong NH, De NV (2012). Development of mitochondrial loop-mediated isothermal amplification for detection of the small liver fluke Opisthorchis viverrini (Opisthorchiidae; Trematoda; Platyhelminthes). J Clin Microbiol, 50, 1178-84. https://doi.org/10.1128/JCM.06277-11
  24. Lemsehow S, HosmerJr WD, Klar JK, Lwanga S (1990). Adequacy of Sample Size in Health Studies. England: John Wiley & Sons;
  25. Miyamoto K, Kirinoki M, Matsuda H, et al (2014). Field survey focused on Opisthorchis viverrini infection in five provinces of Cambodia. Parasitol Int, 63, 366-73. https://doi.org/10.1016/j.parint.2013.12.003
  26. Onsurathum S, Pinlaor P, Haonon O, et al (2016). Effects of fermentation time and low temperature during the production process of Thai pickled fish (pla-som) on the viability and infectivity of Opisthorchis viverrini Int J Food Microbiol, 218, 1-5. https://doi.org/10.1016/j.ijfoodmicro.2015.11.001
  27. Rangsin R, Mungthin M, Taamasri P, et al (2009). Incidence and risk factors of Opisthorchis viverrini infections rural community in Thailand. Am J Trop Med Hyg, 81, 152-5.
  28. Rhongbutsri P, Kitvatanachai S (2002). Survey of the fluke infection rate in Ban Khok Yai village, Khon Kaen, Thailand. J Trop Med Parasitol, 25, 76-8.
  29. Saengsawang P, Promthet S, Bradshaw P (2012). Prevalence of OV infection in Yasothon province, Northeast Thailand . Asian Pac J Cancer Prev, 13, 3399-402. https://doi.org/10.7314/APJCP.2012.13.7.3399
  30. Saengsawang P, Promthet S, Bradshaw P (2013). Infection with Opisthorchis viverrini use of praziquatel among a workingage population in Northeast Thailand. Asian Pacific J Cancer Prev, 14, 2963-6. https://doi.org/10.7314/APJCP.2013.14.5.2963
  31. Sayasone S, Odermatt P, Phoumindr N, et al (2007). Epidemiology of Opisthorchis viverrini in a rural district of southern Lao PDR. Trans R Soc Trop Med Hyg, 101, 40-7. https://doi.org/10.1016/j.trstmh.2006.02.018
  32. Sayasone S, Utzinger J, Akkhavong K, Odermatt P (2015). Repeated stool sampling and use of multiple techniques enhance the sensitivity of helminth diagnosis: a crosssectional survey in southern Lao People’s Democratic Republic. Acta Trop, 141, 315-21. https://doi.org/10.1016/j.actatropica.2014.09.004
  33. Sithithaworn P, Sukavat K, Vanachone B, et al (2006). Epidemiology of food-borne trematodes and other parasite infection in a fishing community on the Nam Ngum reservoir, Lao PDR. Southeast Asian J Trop Med Public Health, 37, 1083-90.
  34. Sithithaworn P, Andrews RH, Nguyen VD, et al (2012). The current status of opisthorchiasis and clonorchiasis in the Mekong Basin. Parasitol Int, 61, 10-6. https://doi.org/10.1016/j.parint.2011.08.014
  35. Sohn WM, Yong TS, Eom KS, et al (2012). Prevalence of Opisthorchis viverrini infection in humans and fish in Kratie Province, Cambodia. Acta Trop, 124, 215-20. https://doi.org/10.1016/j.actatropica.2012.08.011
  36. Sriamporn S, Pisani P, Pipitgool V, et al (2004). Prevalance of Opisthorchis viverrini infection and incidence of cholangiocarcinoma in KhonKaen, Northeast Thailand. Tropical Med Int Health, 9, 588-94. https://doi.org/10.1111/j.1365-3156.2004.01234.x
  37. Sripa B, Bethony JM, Sithithaworn P, et al (2011). Opisthorchiasis and Opisthorchis-associated cholangiocarcinoma in Thailand and Laos. Acta Trop, 120, 158-68. https://doi.org/10.1016/j.actatropica.2010.07.006
  38. Teimoori S, Arimatsu Y, Laha T, et al (2015). Immunodiagnosis of opisthorchiasis using parasite cathepsin F. Parasitol Res, 114, 4571-8. https://doi.org/10.1007/s00436-015-4703-9
  39. Thaewnongiew K, Singthong S, Kutchamart S, et al (2014). Prevalence and risk factors for Opisthorchis viverrini infections in upper Northeast Thailand. Asian Pac J Cancer Prev, 15, 6609-12. https://doi.org/10.7314/APJCP.2014.15.16.6609
  40. Tomokawa S, Kobayashi T, Pongvongsa B, et al (2012). Risk factors for Opisthorchis viverrini infection among schoolchildren in Lao PDR. Southeast Asian J Trop Med Public Health, 43, 574-85.
  41. Wang YC, Feng CC, Sithithaworn P (2013). Environmental determinants of Opisthorchis viverrini prevalence in northeast Thailand. Geospat Health, 8, 111-23. https://doi.org/10.4081/gh.2013.59
  42. Wongratanacheewin S, Pumidonming W, Sermswan R, Maleewong W (2001). Development of a PCR-base method for the detection of Opisthorchis viverrini in experimentally infected hamsters. Parasitol, 122, 175-180.
  43. Worasith C, Kamamia C, Yakovleva A, et al (2015). Advances in the diagnosis of human opisthorchiasis: Development of Opisthorchis viverrini antigen detection in urine. PLoS Negl Trop Dis, 9, e0004157. https://doi.org/10.1371/journal.pntd.0004157
  44. Xayaseng V, Phongluxa K, van Eeuwijk P, Akkhavong K, Odermatt P (2013). Raw fish consumption in liver fluke endemic areas in rural southern Laos. Acta Trop, 127, 105-11. https://doi.org/10.1016/j.actatropica.2013.03.016
  45. Yeoh KW, Promthet S, Sithithaworn P, Kamsa-Ard S, Parkin DM (2015). Re-examination of Opisthorchis viverrini infection in Northeast Thailand. Asian Pac J Cancer Prev, 16, 3413-8. https://doi.org/10.7314/APJCP.2015.16.8.3413
  46. Yong T-S, Shin E-H, Chai J-Y, et al (2012). High prevalence of Opisthorchis viverrini infection in a Riparian population in Takeo Province, Cambodia. Korean J Parasitol, 50, 173-6. https://doi.org/10.3347/kjp.2012.50.2.173

Cited by

  1. Mothers’ Opisthorchis viverrini infection status and raw fish dish consumption in Lao People’s Democratic Republic: determinants of child infection status vol.46, pp.1, 2018, https://doi.org/10.1186/s41182-018-0112-y