Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Mulberry Extract Complex on Degenerative Arthritis In Vivo Models

In Vivo 실험모델에서 오디추출복합물의 퇴행성관절염 개선 효능 연구

  • 이화 ((주)벤스랩 중앙연구소) ;
  • 윤샛별 ((주)벤스랩 중앙연구소) ;
  • 신소희 ((주)벤스랩 중앙연구소) ;
  • 정종문 (수원대학교 생명과학과)
  • Received : 2016.01.12
  • Accepted : 2016.02.25
  • Published : 2016.05.31
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Abstract

The objective of this research was to investigate the in vivo effects of treatment with mulberry extract complex (MEC) on cartilage degeneration and pain severity in an experimental model of rat degenerative arthritis. Monosodium iodoacetate ($2mg/50{\mu}L$) was injected into right knee joints of rats, followed by administration of MEC for 8 weeks at 400 mg/kg or 800 mg/kg of body weight. The experimental data show that treatment with MEC inhibited degradation of glycosaminoglycan and collagen in cartilage. On the other hand, concentrations of cartilage oligomeric matrix protein, C-terminal telopeptide-2, matrix metalloproteinase (MMP)-2, MMP-9, and MMP-13 in serum decreased in comparison with the control. The MEC at all dose levels could inhibit formation of xylene-induced ear edema. In this study, MEC demonstrated significant anti-arthritis activity, which is required for improvement of degenerative arthritis. Based on these results, MEC may be employed for the development of new health foods to ease symptoms of degenerative arthritis.

본 연구에서는 오디추출복합물(mulberry extract complex, MEC)의 퇴행성관절염 증상 완화 및 개선 효과 가능성을 탐색하기 위하여 monosodium iodoacetate(MIA)로 유도한 퇴행성관절염 in vivo 실험모델을 이용하였다. 연골의 주요 구성성분인 glycosaminoglycan(GAG) 및 collagen의 농도를 실험동물의 연골에서 측정한 결과 MIA로 인해 감소하였던 GAG 및 collagen의 농도가 MEC를 경구 투여한 실험군에서 농도 의존적으로 증가하였다. 또한 교원질 합성을 억제하고 분해를 촉진하는 matrix metalloproteinase-2, 9, 13의 농도를 측정한 결과는 MEC의 농도에 따라 감소하는 결과를 보여주었다. 연골 손상 지표인 cartilage oligomeric matrix protein과 C-terminal telopeptide 2의 측정결과에서는 대조군보다 유의성 있는 감소를 나타내어 MEC가 퇴행성관절염의 진행 억제에 도움을 줄 수 있을 것으로 생각된다. 관절염 지수 평가에서도 MEC는 모든 농도에서 대조군보다 유의성 있게 개선되는 결과를 나타내었다. 이상의 실험 결과를 통하여 MEC가 퇴행성관절염에서 나타나는 연골 구성성분의 분해를 억제하고, 여러 중요한 퇴행성관절염 진행 인자를 효과적으로 억제하여 결국 연골파괴 감소와 더불어 통증을 줄여줌으로써 퇴행성관절염에 대한 증상 완화 및 개선 효과가 있을 수 있는 건강기능식품의 원료로 활용될 수 있을 것으로 생각된다.

Keywords

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