Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Genetic Distances in Three Ascidian Species determined by PCR Technique

  • Yoon, Jong-Man (Dept. of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • Received : 2016.10.27
  • Accepted : 2016.12.21
  • Published : 2016.12.31
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Abstract

Seven oligonucleotides primers were shown to generate the shared loci, specific loci, unique shared loci to each species and shared loci by the three species which could be obviously scored. In the present study, 7 oligonucleotides primers produced 401 total loci in the Styela clava (SC) species, 390 in the Halocynthia roretzi (HR) and 434 in the Styela plicata (SP), respectively. Seven oligonucleotides primers generated 275 specific loci in the SC, 341 in the HR and 364 in the SP species, respectively. The oligonucleotides primer BION-23 generated 28 unique loci to each species in the SP species. Especially, the oligonucleotides primer BION-25 produced 7 unique loci to each species, which were identifying each species in the SP species. BION-17 distinguished 21 shared loci by the three ascidian species, major and/or minor fragments of sizes, which were identical in almost all of the samples. Based on the average bandsharing values of all samples, the similarity matrix ranged from 0.519 to 0.774 in the SC species, from 0.261 to 0.683 in the HR species and from 0.346 to 0.730 in the SP species. As regards average bandsharing value (BS) results, individuals from SC species ($0.661{\pm}0.081$) exhibited higher bandsharing values than did individuals from HR species ($0.555{\pm}0.074$) (P<0.05). The dendrogram obtained by the seven oligonucleotides primers indicates three genetic groups. In three ascidian species, the shortest genetic distance (0.071) exhibiting significant molecular difference was also between individual no. 20 and no. 21 within the SP species.

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References

  1. Adams RP (2000) Systematics of the one seeded Juniperus of the eastern hemisphere based on leaf essential oils and random amplified polymorphic DNAs (RAPDs). Biochem Syst Ecol 28:529-543. https://doi.org/10.1016/S0305-1978(99)00096-4
  2. Bartish IV, Garkava LP, Rumpunen K, Nybom H (2000) Phylogenetic relationships and differentiation among and within populations of Chaenomeles Lindl. (Rosaceae) estimated with RAPDs and isozymes. Theor Appl Genet 101:554-563. https://doi.org/10.1007/s001220051515
  3. Cagigas ME, Vazquez E, Blanco G, Sanchez JA (1999) Combined assessment of genetic variability in populations of brown trout (Salmo trutta L.) based on allozymes, microsatellites, and RAPD markers. Mar Biotechnol 1:286-296. https://doi.org/10.1007/PL00011778
  4. Diaz-Jaimes P, Uribe-Alcocer M (2003) Allozyme and RAPD variation in the eastern Pacific yellowfin tuna (Thunnus albacares). Fish Bull 101:769-777.
  5. Guo BZ, Li RG, Widstrom NW, Lynch RE, Cleveland TE (2001) Genetic variation within maize population GT-MAS: gk and the relationship with resistance to Aspergillus flavus and aflatoxin production. Theor Appl Genet 103:533-539. https://doi.org/10.1007/s001220100655
  6. Huh YB, Choi DL (2008) The studies on stability of cultured sea squirt aquaculture fisheries. Report of NIFS. p311.
  7. Islam MS, Ahmed ASI, Azam MS, Alam MS (2005) Genetic analysis of three river populations of Catla catla (HAMILTON) using randomly amplified polymorphic DNAs markers. Asian-Aust J Anim Sci 18:453-457. https://doi.org/10.5713/ajas.2005.453
  8. Iyengar A, Piyapattanakorn S, Dtone DM, Heipel DA, Howell BR, Baynes SM, Maclean N (2000) Identification of microsatellite repeats in turbot (Scophthalmus maximus) and dover sole (Solea solea) using a RAPD-based technique: Characterization of microsatellite markers in dover sole. Mar Biotechnol 2:49-56.
  9. Jeffreys AJ, Morton DB (1987) DNA fingerprints of dogs and cats. Anim Genet 18:1-15.
  10. Lee JH (2011) Development of culture technical for solitary ascidian, Halocynthia aurantium. Report of NIFS. p47.
  11. Mamuris Z, Stamatis C, Bani M, Triantaphyllidis C (1999) Taxonomic relationships between four species of the Mullidae family revealed by three genetic methods: allozymes, random amplified polymorphic DNA and mitochondrial DNA. J Fish Biol 55:572-587. https://doi.org/10.1111/j.1095-8649.1999.tb00700.x
  12. McCormack GC, Powell R, Keegan B (2000) Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidae) with different life history strategies using RAPD markers. Mar Biotechnol 2:100-106.
  13. Nebauer SG, del Castillo-Agudo L, Segura J (2000) An assessment of genetic relationships within the genus Digitalis based on PCR-generated RAPD markers. Theor Appl Genet 100:1209-1216. https://doi.org/10.1007/s001220051426
  14. Nicolosi E, Deng ZN, Gentile A, La Malfa S, Continella G, Tribulato E (2000) Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor Appl Genet 100:1155-1166. https://doi.org/10.1007/s001220051419
  15. Oh H, Yoon JM (2014) Genetic distances of three mollusk species investigated by PCR analysis. Dev Reprod 18(1):43-49. https://doi.org/10.12717/DR.2014.18.1.043
  16. Park SY, Park JS and Yoon JM (2005) Genetic differences and variations in slipper lobster (Ibacus ciliatus) and deep sea lobster (Puerulus sewelli) determined by RAPD analysis. Korean J Genet 25:307-317.
  17. Partis L, Wells RJ (1996) Identification of fish species using random amplified polymorphic DNA (RAPD). Mol Cell Probes 10:435-441. https://doi.org/10.1006/mcpr.1996.0060
  18. Shin YK, Choi NJ, Huh YB, Han HK, Park JH, Kim Y (2007) Survival and Physiological Responses of the Tunicate Halocynthia roretzi to Salinity Changes. J Aqua 20:226-231.
  19. Song YJ, Yoon JM (2013) Genetic differences of three Pollicipes mitella populations identified by PCR analysis. Dev Reprod 17:199-205. https://doi.org/10.12717/DR.2013.17.3.199
  20. Tassanakajon A, Pongsomboon S, Jarayabhand P, Klinbunga S, Boonsaeng V (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. J Mar Biotechnol 6:249-254.
  21. Yoke-Kqueen C, Radu S (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. J Biotechnol 127:161-166. https://doi.org/10.1016/j.jbiotec.2006.06.001
  22. Yoon JM, Kim JY (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analysed by RAPD-PCR. Asian-Aust J Anim Sci 17:1053-1061. https://doi.org/10.5713/ajas.2004.1053