Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Comparative Gene-Expression Analysis of Periodontal Ligament and Dental Pulp in the Human Permanent Teeth

사람 영구치에서 치주인대 및 치수 조직의 유전자 발현에 대한 비교 연구

  • Lee, Suk Woo (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Jeon, Mijeong (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Lee, Hyo-Seol (Department of Pediatric Dentistry, College of Dentistry, Kyunghee University) ;
  • Song, Je Seon (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Son, Heung-Kyu (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Choi, Hyung-Jun (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Jung, Han-Sung (Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Moon, Seok-Jun (Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Park, Wonse (Department of General Dentistry, College of Dentistry, Yonsei University) ;
  • Kim, Seong-Oh (Department of Pediatric Dentistry, College of Dentistry, Yonsei University)
  • 이석우 (연세대학교 치과대학 소아치과학교실) ;
  • 전미정 (연세대학교 치과대학 소아치과학교실) ;
  • 이효설 (경희대학교 치과대학 소아치과학교실) ;
  • 송제선 (연세대학교 치과대학 소아치과학교실) ;
  • 손흥규 (연세대학교 치과대학 소아치과학교실) ;
  • 최형준 (연세대학교 치과대학 소아치과학교실) ;
  • 정한성 (연세대학교 치과대학 구강생물학교실) ;
  • 문석준 (연세대학교 치과대학 구강생물학교실) ;
  • 박원서 (연세대학교 치과대학 통합진료학교실) ;
  • 김성오 (연세대학교 치과대학 소아치과학교실)
  • Received : 2015.05.21
  • Accepted : 2015.10.27
  • Published : 2016.05.31
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Abstract

There is no genetic activity information with the functions of dental pulp and periodontal ligament in human. The purpose of this study was to identify the gene-expression profiles of, and the molecular biological differences between periodontal ligament and dental pulp obtained from human permanent teeth. cDNA microarray analysis identified 347 genes with a fourfold or greater difference in expression level between the two tissue types 83 and 264, of which were more plentiful in periodontal ligament and dental pulp, respectively. Periodontal ligament exhibited strong expression of genes related to collagen synthesis (FAP), collagen degradation (MMP3, MMP9, and MMP13), and bone development and remodeling (SSP1, BMP3, ACP5, CTSK, and PTHLH). Pulp exhibited strong expression of genes associated with calcium ions (CALB1, SCIN, and CDH12) and the mineralization and formation of enamel and dentin (SPARC/SPOCK3, PHEX, AMBN, and DSPP). Among these genes, SPP1, SPARC/SPOCK3, AMBN, and DSPP were well known in dental research. However, the other genes are the newly found and it may help to find a good source of regenerative therapy if further study is performed.

이전 연구에서 사람의 치수와 치주인대의 기능에 대한 구체적인 3만 2천여개의 인체 유전자의 RNA 활성 정보는 없었다. 본 연구의 목적은 사람 영구치에서 얻은 치주인대와 치수 조직 내의 RNA 유전자 발현을 보고하고 각각의 분자생물학적인 차이를 알아보는 것이다. cDNA 미세배열분석에서 두 조직 사이의 유전자 발현 수준에서 4배 이상 차이나는 유전자는 347개로 밝혀졌으며, 치주인대와 치수에서 각각 83개, 264개의 유전자 발현이 4배 이상 차이난다는 것을 보여주었다. 치주인대는 교원질 합성 (FAP), 교원질 분해 (MMP3, MMP9와 MMP13), 골 형성 및 개조 (SPP1, BMP3, ACP5, CTSK와 PTHLH)와 관련된 유전자가 강하게 발현되었다. 반면 치수조직은 칼슘 이온 (CALB1, SCIN와 CDH12)과 법랑질 또는 상아질의 광화 및 형성 (SPARC/SPOCK3, PHEX, AMBN과 DSPP)와 관련한 유전자의 발현이 높게 나타났다. 이들 유전자 중 SPP1, SPARC/SPOCK3, AMBN, DSPP 등의 유전자는 치아의 기능과 관련해서 잘 알려져 있지만, 다른 유전자들은 microarray 분석을 통해서 새롭게 발견된 유전자이다. 이 유전자들은 추가적인 연구가 수행된다면 재생 치료의 좋은 요인을 찾는데 도움이 될 것으로 생각된다.

Keywords

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