Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Phytochemicals from Goniothalamus griffithii Induce Human Cancer Cell Apoptosis

  • Banjerdpongchai, Ratana (Department of Biochemistry, Faculty of Medicine, Chiang Mai University) ;
  • Khaw-on, Patompong (Department of Biochemistry, Faculty of Medicine, Chiang Mai University) ;
  • Pompimon, Wialrt (Laboratory of Natural Products, Faculty of Science, Lampang Rajabhat University)
  • Published : 2016.07.01
⟨ Previous Next ⟩

Abstract

Bioactive compounds extracted from leaves and twigs of Goniothalamus griffithii include pinocembrin (PCN) and goniothalamin (GTN). The objectives of this study were to investigate the cytotoxic activities of PCN and GTN and their influence on molecular signaling for cell death in several human cancer cell lines compared to normal murine fibroblast NIH3T3 cells. GTN exhibited the most potent cytotoxicity against MCF-7 > HeLa > HepG2 > NIH3T3 cells with $IC_{50}$ values of 7.33, 14.8, 37.1 and $65.4{\mu}M$, respectively, whereas PCN was cytotoxic only to HepG2 cells with $IC_{50}$ values of ${\sim}80{\mu}M$. Apoptotic cell death was confirmed by staining the cells with annexin V-FITC and propidium iodide (PI) employing flow cytometry. Apoptosis was shown by externalization of phosphatidylserine in goniothalamin-treated MCF-7 cells in a dose response manner. Positive PI-stained cells with the typical morphology of apoptotic cells were increased dose-dependently. Furthermore, reduction of mitochondrial transmembrane potential was found in goniothalamin-treated MCF-7, HepG2 and HeLa cells. GTN treatment in MCF-7 increased caspase-3, -8 and -9 activities while GTN-induced HeLa cells showed an increase of both caspase-3 and -9 activities. But an increased caspase-8 activity was demonstrated in GTN- and PCN-treated MCF-7 and HepG2 cells, respectively. Taken together, GTN- and PCN-induced human cancer cell apoptosis was through different molecular mechanisms or signaling pathways, which might be due to different machineries in different types of cancer cells, as evidenced by the compound-modulated caspase activities in both intrinsic and/or extrinsic pathways.

Keywords

Acknowledgement

Supported by : Thailand Research Fund (TRF, Commission of Higher Education (CHE)

References

  1. Al-Qubaisi M, Rosli R, Subramani T, et al (2013). Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. Nat Produc Res, 27, 2216-8. https://doi.org/10.1080/14786419.2013.800979
  2. Al-Qubaisi M, Rozita R, Yeap SK, et al (2011). Selective cytotoxicity of goniothalamin against hepatoblastoma HepG2 cells. Molecules, 16, 2944-59. https://doi.org/10.3390/molecules16042944
  3. Alabsi AM, Ali R, Ali AM, et al (2012). Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines. Asian Pac J Cancer Prev, 13, 5131-6. https://doi.org/10.7314/APJCP.2012.13.10.5131
  4. Banjerdpongchai R, Kongtawelert P, Khantamat O, et al (2010). Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells. J Hematol Oncol, 3, 50-65. https://doi.org/10.1186/1756-8722-3-50
  5. Banjerdpongchai R, Punyati P, Nakrob A, et al (2011). 4'-Hydroxycinnamaldehyde from Alpinia galanga (Linn.) induces human leukemic cell apoptosis via mitochondrial and endoplasmic reticulum stress pathways. Asian Pac J Cancer Prev, 12, 593-8.
  6. Banjerdpongchai R, Wudtiwai B, Khaw-On P, et al (2015a). Hesperidin from Citrus seed induces human hepatocellular carcinoma HepG2 cell apoptosis via both mitochondrial and death receptor pathways. Tumour Biol, 21.
  7. Banjerdpongchai R, Wudtiwai B, Pompimon W (2014). Stigmalactam from Orophea enterocarpa induces human cancer cell apoptosis via a mitochondrial pathway. Asian Pac J Cancer Prev, 15, 10397-400.
  8. Banjerdpongchai R, Wudtiwai B, Pompimon W (2015b). Enterocarpam-III induces human liver and breast cancer cell apoptosis via mitochondrial and caspase-9 activation. Asian Pac J Cancer Prev, 16, 1833-7. https://doi.org/10.7314/APJCP.2015.16.5.1833
  9. Banjerdpongchai R, Wudtiwai B, Sringarm K (2013). Cytotoxic and apoptotic-inducing effects of purple rice extracts and chemotherapeutic drugs on human cancer cell lines. Asian Pac J Cancer Prev, 14, 6541-8. https://doi.org/10.7314/APJCP.2013.14.11.6541
  10. Cao G, Ying P, Yan B, et al (2015). Pharmacokinetics, safety, and tolerability of single and multiple-doses of pinocembrin injection administered intravenously in healthy subjects. J Ethnopharmacol, 168, 31-6. https://doi.org/10.1016/j.jep.2015.03.041
  11. Chan KM, Rajab NF, Ishak MH, et al (2006). Goniothalamin induces apoptosis in vascular smooth muscle cells. Chem Biol Interact, 159, 129-40. https://doi.org/10.1016/j.cbi.2005.10.107
  12. Chan KM, Rajab NF, Siegel D, et al (2010). Goniothalamin induces coronary artery smooth muscle cells apoptosis: the p53-dependent caspase-2 activation pathway. Toxicol Sci, 116, 533-48. https://doi.org/10.1093/toxsci/kfq151
  13. Chen WY, Wu CC, Lan YH, et al (2005). Goniothalamin induces cell cycle-specific apoptosis by modulating the redox status in MDA-MB-231 cells. EurJ Pharmacol, 522, 20-9. https://doi.org/10.1016/j.ejphar.2005.08.047
  14. Chiu CC, Liu PL, Huang KJ, et al (2011). Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability. J Agric Food Chem, 59, 4288-93. https://doi.org/10.1021/jf200566a
  15. El-Sharkawy S, Yusuf Z, Pihie AH, Ali AM (1996). Metabolism of goniothalamin in animal and microbial system. Boll Chim Farm, 135, 35-40.
  16. Gao M, Zhu SY, Tan CB, et al (2010). Pinocembrin protects the neurovascular unit by reducing inflammation and extracellular proteolysis in MCAO rats. J Asian Nat Prod Res, 12, 407-18. https://doi.org/10.1080/10286020.2010.485129
  17. Inayat-Hussain SH, Chan KM, Rajab NF, et al (2010). Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells. Toxicol Lett, 193, 108-14. https://doi.org/10.1016/j.toxlet.2009.12.010
  18. Inayat-Hussain SH, Osman AB, Din LB, et al (1999). Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells. FEBS Lett, 456, 379-83. https://doi.org/10.1016/S0014-5793(99)00984-9
  19. Kumar MA, Nair M, Hema PS, et al (2007). Pinocembrin triggers Bax-dependent mitochondrial apoptosis in colon cancer cells. Mol Carcinog, 46, 231-41. https://doi.org/10.1002/mc.20272
  20. Kuo KK, Chen YL, Chen LR, et al (2011). Involvement of phorbol-12-myristate-13-acetate-induced protein 1 in goniothalamin-induced TP53-dependent and -independent apoptosis in hepatocellular carcinoma-derived cells. Toxicol Appl Pharmacol, 256, 8-23. https://doi.org/10.1016/j.taap.2011.07.002
  21. Lan X, Wang W, Li Q, Wang J (2015). The Natural Flavonoid Pinocembrin: Molecular Targets and Potential Therapeutic Applications. Mol Neurobiol, 53, 1794-801.
  22. Li LK, Rola AS, Kaid FA, et al (2016). Goniothalamin induces cell cycle arrest and apoptosis in H400 human oral squamous cell carcinoma: A caspase-dependent mitochondrialmediated pathway with downregulation of NF-kappabeta. Arch Oral Biol, 64, 28-38. https://doi.org/10.1016/j.archoralbio.2015.12.002
  23. Mosaddik MA, Haque ME (2003). Cytotoxicity and antimicrobial activity of goniothalamin isolated from Bryonopsis laciniosa. Phytother Res, 17, 1155-7. https://doi.org/10.1002/ptr.1303
  24. Mu Q, Tang WD, Liu RY, et al (2003). Constituents from the stems of Goniothalamus griffithii. Planta Med, 69, 826-30. https://doi.org/10.1055/s-2003-43219
  25. Petsophonsakul P, Pompimon W, Banjerdpongchai R (2013). Apoptosis induction in human leukemic promyelocytic HL-60 and monocytic U937 cell lines by goniothalamin. Asian Pac J Cancer Prev, 14, 2885-9. https://doi.org/10.7314/APJCP.2013.14.5.2885
  26. Saad MA, Abdel Salam RM, Kenawy SA, Attia AS (2015). Pinocembrin attenuates hippocampal inflammation, oxidative perturbations and apoptosis in a rat model of global cerebral ischemia reperfusion. Pharmacol Rep, 67, 115-22. https://doi.org/10.1016/j.pharep.2014.08.014
  27. Sophonnithiprasert T, Mahabusarakam W, Nakamura Y, Watanapokasin R (2015a). Antiproliferation and Apoptosis Induction in Colorectal Cancer Cells by Goniothalamin. J Med Assoc Thai, 98, 146-51.
  28. Sophonnithiprasert T, Nilwarangkoon S, Nakamura Y, Watanapokasin R (2015b). Goniothalamin enhances TRAILinduced apoptosis in colorectal cancer cells through DR5 upregulation and cFLIP downregulation. Int J Oncol, 47, 2188-96. https://doi.org/10.3892/ijo.2015.3204
  29. Trakoontivakorn G, Nakahara K, Shinmoto H, et al (2001). Structural analysis of a novel antimutagenic compound, 4-Hydroxypanduratin A, and the antimutagenic activity of flavonoids in a Thai spice, fingerroot (Boesenbergia pandurata Schult.) against mutagenic heterocyclic amines. J Agric Food Chem, 49, 3046-50. https://doi.org/10.1021/jf010016o
  30. Umar-Tsafe N, Mohamed-Said MS, Rosli R, et al (2004). Genotoxicity of goniothalamin in CHO cell line. Mutat Res, 562, 91-102. https://doi.org/10.1016/j.mrgentox.2004.05.011
  31. Wu CX, Liu R, Gao M, et al (2013). Pinocembrin protects brain against ischemia/reperfusion injury by attenuating endoplasmic reticulum stress induced apoptosis. Neurosci Lett, 546, 57-62. https://doi.org/10.1016/j.neulet.2013.04.060
  32. Yen CY, Chiu CC, Haung RW, et al (2012). Antiproliferative effects of goniothalamin on Ca9-22 oral cancer cells through apoptosis, DNA damage and ROS induction. Mutat Res, 747, 253-8. https://doi.org/10.1016/j.mrgentox.2012.06.003
  33. Zhao G, Zhang W, Li L, et al (2014). Pinocembrin protects the brain against ischemia-reperfusion injury and reverses the autophagy dysfunction in the penumbra area. Molecules, 19, 15786-98. https://doi.org/10.3390/molecules191015786
  34. Zhou FS, Tang WD, Mu Q, et al (2005). Semisynthesis and antitumor activities of new styryl-lactone derivatives. Chem Pharmaceut Bull, 53, 1387-91. https://doi.org/10.1248/cpb.53.1387